Isolasi dan Uji Aktivitas Antioksidan Metabolit Sekunder dari Ekstrak Etil Asetat Kulit Batang Kangkang Katup (Bauhinia semibifida Roxb)

Al-Kimia Pub Date : 2020-06-29 DOI:10.24252/al-kimia.v8i1.10152

Haiyul Fadhli, Anita Lukman, R. Adawiyah

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Abstract

Antioxidant was a compound which possessed an activity as scavenger of free radicals. This study was aimed to examine the antioxidant activity of ethyl acetate fraction and isolated compound from stem barks of Bauhinia semibifida Roxb. Samples extracted by gradual maceration with n-hexane solvent, ethyl acetate and methanol respectively. Isolation was carried out using Vacuum Liquid Chromatrography and purification by column chromatography with Sephadex LH-20. Isolated compound (BS) obtained was ivory crystalline with melting point 163-165 0 C. The analysis of FT-IR spectra, UV spectra and the result of qualitative test by Liebermann Burchard reagent were is shown positive as a terpenoid. Antioxidant activity were evaluated by using DPPH method for ethyl acetate fraction and isolation compound in serial concentration of 1000; 500; 250; 125; 62,5; 31,25 ug/mL. The result found that ethyl acetate fraction (f4 and f11) of stem barks of Bauhinia semibifida showed antioxidant activity with IC 50 value of >1000 ug/mL; 179 ug/mL. In conclusion, F11 from ethyl acetate extract from stem barks of Bauhinia semibifida Roxb. exhibited an activity as scavenger of free radicals from DPPH. Keywords: A ntioxidant, Bauhinia semibifida Roxb , IC 50 , VLC

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半裂紫荆（Bauhinia semilifida Roxb）乙基提取物Astate皮肤阀座次生抗氧化活性代谢产物的分离与检测

抗氧化剂是一种具有清除自由基活性的化合物。本研究旨在检测半裂紫荆茎皮中乙酸乙酯部分和分离化合物的抗氧化活性。样品分别用正己烷溶剂、乙酸乙酯和甲醇逐步浸渍提取。用真空液相色谱法进行分离，用Sephadex LH-20柱色谱法纯化。分离得到的化合物（BS）为象牙状结晶，熔点163-165℃。FT-IR光谱、UV光谱分析和Lieberman-Burchard试剂的定性测试结果显示，其为萜类化合物。采用DPPH法对串联浓度为1000的乙酸乙酯组分和分离化合物进行抗氧化活性评价；500；250；125；62,5；31,25微克/毫升。结果表明，半裂紫荆茎皮乙酸乙酯组分（f4和f11）具有抗氧化活性，IC50值>1000ug/mL；179微克/毫升。综上所述，从紫荆茎皮中提取乙酸乙酯F11。表现出作为DPPH的自由基清除剂的活性。关键词：抗氧化剂，半裂紫荆Roxb，IC50，VLC

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Al-Kimia

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24 weeks