Highly sensitive glucose electrochemical sensor using sugar-lectin interactions

Abstract

In this study, glucose oxidase (GOx) was immobilized on the electrode surface by layer-by-layer and gel membrane technique and characterized the GOx immobilized film morphology, H₂O₂ permeability, and glucose response. Concanavalin A (Con A)-GOx multilayer electrodes showed higher glucose-related current response than GOx-bovine serum albumin gel membrane-coated electrode, a common modification method. The thin thickness of the Con A/GOx multilayer film efficiently catalyzed the enzymatic reaction, and H₂O₂ was produced near the electrode surface, resulting in an immediate electrode response.