Effects of glycyrrhizic acid (Viusid-Vet® powder) on the reduction of influenza virus spread and on production parameters in pigs

IF 0.2 4区 农林科学 Q2 Veterinary Veterinaria Mexico Pub Date : 2017-03-22 DOI:10.21753/VMOA.4.1.373
L. Ocampo-Camberos, G. Tapia, L. Gutiérrez, H. Sumano-López
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引用次数: 5

Abstract

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Ocampo Camberos L, Tapia G, Gutierrez L, Sumano Lopez HS. Effects of glycyrrhizic acid (Viusid-Vet® powder) on the reduction of influenza virus spread and on production parameters in pigs. Veterinaria Mexico OA. 2017;4(1). doi: 10.21753/vmoa.4.1.373 Influenza viruses are among the most important respiratory pathogens in pigs and humans. They cause seasonal epidemics in pigs and occasional pandemics in humans. Herbal remedies have been regarded as suitable elements to aid in controlling influenza. This study was carried out to analyse the effects of the in-feed administration of glycyrrhizic acid, the best-known component of liquorice (as Viusid-Vet® powder), in pigs suffering an outbreak of influenza. Eighty crossbred Duroc-Landrace pigs, one day post-weaning (22 days old), were included in this trial. Piglets were randomly divided into the following two groups: those treated with glycyrrhizic acid and an untreated control group. Serological measurements to assess viral load and humoral responses were carried out. Blood samples from pigs were obtained every fortnight, starting on week two and ending on week 15. With these samples, haemagglutination inhibition (HI) tests were performed, using A/swine/New Jersey/11/76 (H1N1) and A/swine/Minnesota/9088-2/98 (H3N2) as reference viruses. Quantitative RT-PCR tests against the M gene of the influenza virus were also performed to assess viral shedding from nasal swab samples on weeks 1 to 8 after the beginning of the trial. Weight variables were assessed weekly for 18 weeks. In the HI tests, treated animals showed fewer positive responses compared to the control group for H1N1 and H3N2. However, a positive response to viral protection, as assessed by HI tests, was regarded as not conclusive of humoral immune stimulation. qRT-PCR tests for viral spread exhibited a lower rate of excretion for the treated group compared to the untreated one. Hence, it appears that glycyrrhizic acid stimulates, to some extent, immune responses against pig influenza as measured by viral shedding. For mean body weight, the generalized estimating equations show a higher weight gain for pigs treated with glycyrrhizic acid than the control group (P = 0.0001). These effects may assist producers in addressing the aftermath of an influenza outbreak. Figure 1. Results of RT-PCR viral shedding test against the M gene. (Likelihood χ21,7 = 19.2 P = 0.0001).
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甘草酸(Viusid-Vet®粉末)对猪流感病毒传播和生产参数的影响
引用本文为:Ocampo Camberos L, Tapia G, Gutierrez L, Sumano Lopez HS。甘草酸(Viusid-Vet®粉末)对猪流感病毒传播和生产参数的影响。墨西哥兽医局。2017; 4(1)。流感病毒是猪和人类最重要的呼吸道病原体之一。它们在猪中引起季节性流行病,偶尔在人类中引起流行病。草药已被认为是帮助控制流感的适当元素。本研究的目的是分析在猪流感爆发时,在饲料中添加甘草最著名的成分甘草酸(作为Viusid-Vet®粉末)的效果。试验选用断奶后1 d(22日龄)的杜洛克-长白杂交猪80头。仔猪随机分为两组:甘草酸处理组和未处理对照组。进行血清学测量以评估病毒载量和体液反应。从第2周开始至第15周结束,每两周采集一次猪的血液样本。以A/swine/New Jersey/11/76 (H1N1)和A/swine/Minnesota/9088-2/98 (H3N2)为参比病毒,进行血凝抑制试验。还进行了针对流感病毒M基因的定量RT-PCR试验,以评估试验开始后第1至8周鼻拭子样本中的病毒脱落情况。每周评估体重变量,持续18周。在HI测试中,与对照组相比,治疗动物对H1N1和H3N2的阳性反应较少。然而,根据HI试验的评估,对病毒保护的阳性反应被认为不是体液免疫刺激的结论性结果。病毒传播的qRT-PCR测试显示,与未治疗组相比,治疗组的排泄率较低。因此,甘草酸似乎在某种程度上刺激了对猪流感的免疫反应,这是通过病毒脱落来衡量的。对于平均体重,广义估计方程显示,甘草酸组猪的增重高于对照组(P = 0.0001)。这些影响可能有助于生产者处理流感爆发的后果。图1所示。M基因RT-PCR病毒脱落试验结果。(似然χ21,7 = 19.2 P = 0.0001)。
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来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
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>12 weeks
期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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