Beneficial effects of gymnemic acid on three-dimensional vascular architecture and expression of vascular endothelial growth factor of intrarenal segmental and interlobar arteries in diabetic rat kidney

IF 1.3 Q4 FOOD SCIENCE & TECHNOLOGY Functional Foods in Health and Disease Pub Date : 2022-06-22 DOI:10.31989/ffhd.v12i6.930
Manaras Komolkriengkrai, Rawipa Jangchart, Nichawadee Sandech, Uraporn Vongvatcharanon, W. Khimmaktong
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引用次数: 2

Abstract

Background: A high prevalence of atherosclerotic vascular lesions has been associated with renal disease and diabetes and is a major cause for increasing deaths from cardiovascular disease. The present study aimed to determine the beneficial effects of gymnemic acids on the kidney microvasculature and to establish their anti-angiogenic properties that are related to the expression of vascular endothelial growth factor (VEGF) protein of segmental and interlobar arteries in induced diabetic rats. Methods: Rats were divided into five groups including the control group (C), control treated with gymnemic acid (CGM), diabetic animals (DM group) that were rendered diabetic by a single dose [60 mg/kg body weight (BW)] of a streptozotocin (STZ) injection, diabetic rats treated with gymnemic acid (400 mg/kg BW) (GM), and diabetic rats treated with glibenclamide (4 mg/kg BW) (GR). After 8 weeks, kidney tissues were collected for histological analysis. In rats with DM, the segmental arteries exhibited increased wall thickness. The kidney microvasculature was examined using the vascular corrosion casting method. Results: Rats with DM presented a decreasing diameter of segmental and interlobar arteries. They were evidently redeveloped and restored in the GM and GR groups. As determined by immunofluorescence, the expression of VEGF was significantly reduced in both the GM and GR groups. Conclusions: The present study demonstrated that gymnemic acid from Gymnemasylvestre may be a promising medical herb for use in the treatment of diabetes and kidney disease.Keywords: diabetes mellitus, segmental artery, interlobar artery, gymnemic acid, vascular architecture
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裸子酸对糖尿病大鼠肾内节段动脉和叶间动脉三维血管结构及血管内皮生长因子表达的有益影响
背景:动脉粥样硬化性血管病变的高发与肾脏疾病和糖尿病有关,并且是心血管疾病死亡增加的主要原因。本研究旨在确定裸子酸对糖尿病大鼠肾脏微血管的有益作用,并确定其抗血管生成特性与节段动脉和叶间动脉血管内皮生长因子(VEGF)蛋白的表达有关。方法:将大鼠分为5组,分别为对照组(C)、裸子藤酸(CGM)治疗组、单剂量[60 mg/kg体重(BW)]链脲佐菌素(STZ)治疗组、裸子藤酸(400 mg/kg体重(GM))治疗组和格列本脲(4 mg/kg体重(GR)治疗组。8周后,收集肾组织进行组织学分析。DM大鼠的节段动脉壁厚增加。采用血管腐蚀铸造法对肾脏微血管进行了检测。结果:DM大鼠表现为节段动脉和叶间动脉直径减小。在GM和GR组,它们明显得到了重新发育和恢复。免疫荧光检测结果显示,GM组和GR组VEGF的表达均显著降低。结论:本研究表明,从金缕草中提取的金缕草酸可能是一种治疗糖尿病和肾脏疾病的有前途的草药。关键词:糖尿病,节段动脉,叶间动脉,裸子酸,血管结构
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来源期刊
Functional Foods in Health and Disease
Functional Foods in Health and Disease FOOD SCIENCE & TECHNOLOGY-
CiteScore
2.20
自引率
20.00%
发文量
47
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