Inhibition Test of Browning Reaction in Apple (Malus domestica Borkh.) by Low NaCl Concentration

M. Yusuf, A. Legowo, A. N. Al-Baarri
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Abstract

Reaction of enzymatic browning in fruit is a reaction that occurs due to the oxidation of phenol and involves the enzyme polyphenol oxidase (PPO). Prevention of browning reactions can be done by inhibiting PPO activity, one of alternative is to use NaCl. The purpose of this study was to determine the ability of low NaCl concentration to inhibit the browning reaction based on the color change of L* value. Apple peels was dipped in phosphate buffer pH 7.0 containing 0.1 M NaCl and incubated at room temperature for 6 hours. The result indicated the hindrance of NaCl in the development browning color that was detected by L* value. In conclusion, NaCl may inhibit the process of browning apple in room temperature.
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低NaCl浓度对苹果褐变反应的抑制试验
水果中的酶促褐变反应是由于苯酚的氧化而发生的反应,涉及多酚氧化酶(PPO)。可以通过抑制PPO活性来防止褐变反应,其中一种选择是使用NaCl。本研究的目的是根据L*值的颜色变化来确定低NaCl浓度抑制褐变反应的能力。将苹果皮浸入含有0.1M NaCl的pH 7.0的磷酸盐缓冲液中,并在室温下孵育6小时。结果表明,用L*值检测NaCl对褐变的影响。综上所述,NaCl可以抑制苹果在室温下褐变的过程。
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