Nichcha Nitthikan, P. Leelapornpisid, Ornchuma Naksuriya, Nutjeera Intasai, Kanokwan Kiattisin
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引用次数: 8
Abstract
This study aimed to investigate the ability of brown Agaricus bisporus extracts to enhance xerosis treatment via their biological activities, including their antioxidant, anti-aging, and anti-inflammation. Brown A. bisporus ethanol extract (EE) and brown A. bisporus water extract (WE) contained ergothioneine and gallic acid as their major compounds, as detected by HPLC, respectively. The WE exhibited the highest total polysaccharide content (734.04 ± 0.03 mg glucose/g extract) and total phenolic content (190.90 ± 0.07 mg gallic acid/g extract). The WE exhibited an inhibitory effect of 83.34 ± 18.66% on a collagenase enzyme, whereas the EE inhibited the elastase enzymes by 81.26 ± 4.37%. In addition, the EE also demonstrated strong activities against DPPH, with an IC50 0.30 ± 0.04 mg/mL, ABTS with a TEAC value of 8.06 ± 0.08 µM Trolox/g extract, and a FRAP assay with a FRAP value of 390.50 ± 0.32 mM FeSO4/g. In addition, all extracts were non-cytotoxic and could decrease the secretion of IL-6 and TNF-α in HaCaT cells. Therefore, brown A. bisporus extracts might be a potential natural raw material that can be further used in cosmeceutical products for xerosis treatment due to their good efficacy.
本研究旨在研究双孢蘑菇提取物通过其抗氧化、抗衰老和抗炎等生物活性增强干燥症治疗的能力。用高效液相色谱法检测，双孢棕乙醇提取物（EE）和双孢棕水提取物（WE）分别含有麦角硫酮和没食子酸作为主要化合物。WE表现出最高的总多糖含量（734.04±0.03mg葡萄糖/g提取物）和总酚含量（190.90±0.07mg没食子酸/g提取物）。WE对胶原酶表现出83.34±18.66%的抑制作用，而EE对弹性蛋白酶的抑制作用为81.26±4.37%。此外，EE对DPPH也表现出强大的活性，IC50为0.30±0.04 mg/mL，ABTS的TEAC值为8.06±0.08µM Trolox/g提取物，FRAP值为390.50±0.32 mM FeSO4/g。此外，所有提取物均无细胞毒性，可降低HaCaT细胞中IL-6和TNF-α的分泌。因此，棕色双孢蘑菇提取物可能是一种潜在的天然原料，由于其良好的疗效，可进一步用于治疗干燥症的药妆产品。
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