首页 > 最新文献

Scientia Pharmaceutica最新文献

英文 中文
Advances in the Production of PBCA Microparticles Using a Micromixer with HH-Geometry in a Microfluidic System 在微流体系统中使用具有 HH 几何结构的微混合器生产 PBCA 微颗粒的进展
IF 2.3 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-08-09 DOI: 10.3390/scipharm92030043
Aline Rocha Vieira, Aline Furtado Oliveira, Fabiana Vieira Lima Solino Pessoa, Beatriz Nogueira Messias de Miranda, A. Baby
Different reaction control methods for producing nano/microstructures of poly(butyl cyanoacrylate) (PBCA) have been studied, focusing on pH and monomer-to-initiator ratios. However, these methods often require multiple steps and reagents. In the synthesis of PBCA microparticles using three versions of micromixers designed with HH geometry and varying numbers of channels (4, 10, and 15), different synthesis formulations were investigated by varying monomer concentrations. PBCA microparticles synthesized with 19.2% (w/w) n-butyl cyanoacrylate (n-BCA) monomer, a residence time of 0.06 s, a flow rate of 78 mL·min−1, and a phase ratio of 45/55 (W/O), exhibited an average diameter of 642.2 nm as determined by dynamic light scattering (DLS) analysis. In contrast, PBCA microparticles synthesized with 5.0% (w/w) n-BCA monomer, the same residence time of 0.06 s, a flow rate of 39 mL·min−1, and a phase ratio of 20/80 (W/O), exhibited an average diameter of 74.73 µm according to laser diffraction particle size analysis. Polymer formation was confirmed by Fourier-transform infrared (FTIR) spectroscopy in both formulation and process conditions. These results indicate that the parameters for the production of microparticles with different monomer concentrations in the microfluidic system with HH geometry and 15 channels can be optimized for potential applications in cosmetics and pharmaceutical ingredients.
人们研究了不同的反应控制方法来生产聚(氰基丙烯酸丁酯)(PBCA)的纳米/微结构,重点是 pH 值和单体与引发剂的比例。然而,这些方法往往需要多个步骤和试剂。在使用三种版本的微搅拌器合成 PBCA 微颗粒的过程中,设计了 HH 几何形状和不同数量的通道(4、10 和 15),并通过改变单体浓度研究了不同的合成配方。经动态光散射(DLS)分析测定,用 19.2%(w/w)氰基丙烯酸正丁酯(n-BCA)单体、0.06 秒的停留时间、78 mL-min-1 的流速和 45/55 (W/O)的相比合成的 PBCA 微颗粒的平均直径为 642.2 nm。相比之下,用 5.0% (重量比)n-BCA 单体、相同的 0.06 秒停留时间、39 mL-min-1 的流速和 20/80 (W/O)的相比合成的 PBCA 微颗粒,根据激光衍射粒度分析,平均直径为 74.73 µm。傅立叶变换红外光谱(FTIR)证实了在两种配方和工艺条件下聚合物的形成。这些结果表明,在具有 HH 几何形状和 15 个通道的微流体系统中生产不同单体浓度的微颗粒的参数可以得到优化,从而有可能应用于化妆品和药物成分中。
{"title":"Advances in the Production of PBCA Microparticles Using a Micromixer with HH-Geometry in a Microfluidic System","authors":"Aline Rocha Vieira, Aline Furtado Oliveira, Fabiana Vieira Lima Solino Pessoa, Beatriz Nogueira Messias de Miranda, A. Baby","doi":"10.3390/scipharm92030043","DOIUrl":"https://doi.org/10.3390/scipharm92030043","url":null,"abstract":"Different reaction control methods for producing nano/microstructures of poly(butyl cyanoacrylate) (PBCA) have been studied, focusing on pH and monomer-to-initiator ratios. However, these methods often require multiple steps and reagents. In the synthesis of PBCA microparticles using three versions of micromixers designed with HH geometry and varying numbers of channels (4, 10, and 15), different synthesis formulations were investigated by varying monomer concentrations. PBCA microparticles synthesized with 19.2% (w/w) n-butyl cyanoacrylate (n-BCA) monomer, a residence time of 0.06 s, a flow rate of 78 mL·min−1, and a phase ratio of 45/55 (W/O), exhibited an average diameter of 642.2 nm as determined by dynamic light scattering (DLS) analysis. In contrast, PBCA microparticles synthesized with 5.0% (w/w) n-BCA monomer, the same residence time of 0.06 s, a flow rate of 39 mL·min−1, and a phase ratio of 20/80 (W/O), exhibited an average diameter of 74.73 µm according to laser diffraction particle size analysis. Polymer formation was confirmed by Fourier-transform infrared (FTIR) spectroscopy in both formulation and process conditions. These results indicate that the parameters for the production of microparticles with different monomer concentrations in the microfluidic system with HH geometry and 15 channels can be optimized for potential applications in cosmetics and pharmaceutical ingredients.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141922447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phytochemical Profiling and Biological Activities of Two Helianthemum Species Growing in Greece 生长在希腊的两种太阳花的植物化学成分分析和生物活性
IF 2.3 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-08-08 DOI: 10.3390/scipharm92030042
Evgenia Panou, K. Graikou, N. Tsafantakis, Fanourios-Nikolaos Sakellarakis, I. Chinou
Helianthemum nummularium (HN) and Helianthemum oelanticum subsp. incanum (HO) are plant species, among Cistaceae, that are highly distributed in the Mediterranean region. In the current study, extracts of the aerial parts from both species have been analyzed phytochemically. The non-polar extract analysis resulted in the identification of 15 compounds in each species, mainly terpene and fatty acid derivatives, through GC–MS. The methanolic extract analysis, conducted through UHPLC–MS/MS, led to the identification of 39 metabolites in HN and 29 in HO, respectively, the majority of which were phenolics. Among the identified compounds, several have also been isolated and structurally determined (from HN: rutin, linoleic acid, gallic acid, and isoquercetin, and from HO: quercetin-3-O-(2″-O-galloyl)-galactopyranoside, methyl gallate, catechin-3-O-glucopyranoside, and astragalin, while hyperoside, and cis- and trans-tiliroside have been determined in both species). Furthermore, the methanolic extracts of HN and HO displayed a high total phenolic content (177.2 mg GA/g extract and 150.6 mg GA/g extract, respectively) and considerable free-radical scavenging activity against the DPPH radical (94.6% and 94.0% DPPH inhibition, respectively). Antimicrobial testing showed stronger inhibition of HN against Gram (+) bacterial strains (MIC values 0.07–0.15 mg/mL), while both extracts exhibited low tyrosinase-inhibitory activity. Considering the lack of studies conducted on the chemistry and biological activities of the genus Helianthemum, the chemical characterization of extracts could contribute to new sources of bioactive metabolites to be explored and exploited for further potential applications such as food and/ or the cosmetic industry.
Helianthemum nummularium(HN)和 Helianthemum oelanticum subsp. incanum(HO)属于肉苁蓉科植物,在地中海地区分布广泛。本研究对这两种植物的气生部分提取物进行了植物化学分析。通过气相色谱-质谱(GC-MS)对非极性萃取物的分析,确定了两种植物中的 15 种化合物,主要是萜烯和脂肪酸衍生物。通过超高效液相色谱-质谱/质谱对甲醇提取物进行分析,在 HN 和 HO 中分别鉴定出 39 种代谢物和 29 种代谢物,其中大部分是酚类化合物。在鉴定出的化合物中,有几种还被分离出来并确定了结构(从 HN 中:芦丁、亚油酸、没食子酸和异槲皮素;从 HO 中:槲皮素-3-O-(2″-O-谷酰基)-吡喃半乳糖苷、没食子酸甲酯、儿茶素-3-O-吡喃葡萄糖苷和黄芪苷,而在这两种植物中都确定了金丝桃苷、顺式和反式枸橘苷)。此外,HN 和 HO 的甲醇提取物显示出较高的总酚含量(分别为 177.2 毫克 GA/g 提取物和 150.6 毫克 GA/g 提取物)和相当高的 DPPH 自由基清除活性(DPPH 抑制率分别为 94.6% 和 94.0%)。抗菌测试表明,HN 对革兰氏(+)细菌菌株有较强的抑制作用(MIC 值为 0.07-0.15 mg/mL),而这两种提取物对酪氨酸酶的抑制活性较低。考虑到缺乏对鹤顶红属植物化学和生物活性的研究,提取物的化学特征描述有助于探索生物活性代谢物的新来源,并将其进一步应用于食品和/或化妆品行业。
{"title":"Phytochemical Profiling and Biological Activities of Two Helianthemum Species Growing in Greece","authors":"Evgenia Panou, K. Graikou, N. Tsafantakis, Fanourios-Nikolaos Sakellarakis, I. Chinou","doi":"10.3390/scipharm92030042","DOIUrl":"https://doi.org/10.3390/scipharm92030042","url":null,"abstract":"Helianthemum nummularium (HN) and Helianthemum oelanticum subsp. incanum (HO) are plant species, among Cistaceae, that are highly distributed in the Mediterranean region. In the current study, extracts of the aerial parts from both species have been analyzed phytochemically. The non-polar extract analysis resulted in the identification of 15 compounds in each species, mainly terpene and fatty acid derivatives, through GC–MS. The methanolic extract analysis, conducted through UHPLC–MS/MS, led to the identification of 39 metabolites in HN and 29 in HO, respectively, the majority of which were phenolics. Among the identified compounds, several have also been isolated and structurally determined (from HN: rutin, linoleic acid, gallic acid, and isoquercetin, and from HO: quercetin-3-O-(2″-O-galloyl)-galactopyranoside, methyl gallate, catechin-3-O-glucopyranoside, and astragalin, while hyperoside, and cis- and trans-tiliroside have been determined in both species). Furthermore, the methanolic extracts of HN and HO displayed a high total phenolic content (177.2 mg GA/g extract and 150.6 mg GA/g extract, respectively) and considerable free-radical scavenging activity against the DPPH radical (94.6% and 94.0% DPPH inhibition, respectively). Antimicrobial testing showed stronger inhibition of HN against Gram (+) bacterial strains (MIC values 0.07–0.15 mg/mL), while both extracts exhibited low tyrosinase-inhibitory activity. Considering the lack of studies conducted on the chemistry and biological activities of the genus Helianthemum, the chemical characterization of extracts could contribute to new sources of bioactive metabolites to be explored and exploited for further potential applications such as food and/ or the cosmetic industry.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141929710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Implementation of an Antimicrobial Stewardship Program at the Hospital and ICU Level of a Clinic in Sincelejo-Sucre 在索斯雷霍-苏克雷一家诊所的医院和重症监护室实施抗菌药物管理计划
IF 2.3 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-17 DOI: 10.3390/scipharm92030039
Erick Diaz-Morales, Ana Paola Pacheco-Hermosilla, Daniel Esteban Castro-Mangonez, Nerlys Pajaro-Castro
Objectives: In this retrospective observational study, the aim is to establish how the implementation of the use of antimicrobial stewardship programs at the hospital and intensive care unit level in a Sucre Clinic in Sincelejo has had a significant impact on the improvement of the rational use of antibiotics, due to the alarming situation of increasing antibiotic resistance. Materials and methods: The methodology used was to analyze the Excel database of the Clinic in such a way as to compare the data from 2017, the period prior to the implementation of the antimicrobial stewardship program (ASP), with the subsequent evolution between the years 2018 and 2022, in relation to the institutional records of four antibiotics—ceftriaxone3, ciprofloxacin4, meropenem5, and vancomycin6, measured in defined daily dose (DDD). Results: According to the defined daily dose values obtained for the four antibiotics, a reduction in the defined daily dose was identified in the post-implementation period. On the other hand, considering the DDD reported by the World Health Organization for each of the antibiotics, significant differences were verified in comparison with those obtained in the clinic in the hospitalization and intensive care unit services. Conclusions: In conclusion, in the clinic, a reduction in the defined daily dose was verified in the period after the implementation of the antimicrobial stewardship program compared to the previous period, both in the hospitalization and intensive care unit, as well as having a mild-to-large effect with Cohen’s D.
研究目的在这项回顾性观察研究中,由于抗生素耐药性不断增加的情况令人担忧,因此旨在确定在自莱霍的一家苏克雷诊所的医院和重症监护室实施抗菌药物管理计划对改善抗生素的合理使用产生了怎样的重大影响。材料与方法采用的方法是分析该诊所的 Excel 数据库,以比较 2017 年(即抗菌药物管理计划(ASP)实施之前)的数据与 2018 年至 2022 年期间的后续演变,这些数据与四种抗生素--头孢曲松3、环丙沙星4、美罗培南5 和万古霉素6--的机构记录有关,以确定的每日剂量(DDD)来衡量。结果:根据获得的四种抗生素的规定日剂量值,发现在实施后规定日剂量有所减少。另一方面,考虑到世界卫生组织报告的每种抗生素的 DDD 值,在住院和重症监护室服务中,与在诊所获得的 DDD 值相比,证实存在显著差异。结论总之,抗菌药物管理计划实施后,在临床中,无论是住院治疗还是重症监护室服务,与之前相比,确定的日剂量都有所减少,并且在科恩氏D上也有轻度到重度的影响。
{"title":"Implementation of an Antimicrobial Stewardship Program at the Hospital and ICU Level of a Clinic in Sincelejo-Sucre","authors":"Erick Diaz-Morales, Ana Paola Pacheco-Hermosilla, Daniel Esteban Castro-Mangonez, Nerlys Pajaro-Castro","doi":"10.3390/scipharm92030039","DOIUrl":"https://doi.org/10.3390/scipharm92030039","url":null,"abstract":"Objectives: In this retrospective observational study, the aim is to establish how the implementation of the use of antimicrobial stewardship programs at the hospital and intensive care unit level in a Sucre Clinic in Sincelejo has had a significant impact on the improvement of the rational use of antibiotics, due to the alarming situation of increasing antibiotic resistance. Materials and methods: The methodology used was to analyze the Excel database of the Clinic in such a way as to compare the data from 2017, the period prior to the implementation of the antimicrobial stewardship program (ASP), with the subsequent evolution between the years 2018 and 2022, in relation to the institutional records of four antibiotics—ceftriaxone3, ciprofloxacin4, meropenem5, and vancomycin6, measured in defined daily dose (DDD). Results: According to the defined daily dose values obtained for the four antibiotics, a reduction in the defined daily dose was identified in the post-implementation period. On the other hand, considering the DDD reported by the World Health Organization for each of the antibiotics, significant differences were verified in comparison with those obtained in the clinic in the hospitalization and intensive care unit services. Conclusions: In conclusion, in the clinic, a reduction in the defined daily dose was verified in the period after the implementation of the antimicrobial stewardship program compared to the previous period, both in the hospitalization and intensive care unit, as well as having a mild-to-large effect with Cohen’s D.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141829393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combined Effect of Sertraline and Capecitabine on Breast Cancer Cell Lines In Vitro and In Silico Evidence for Synergistic Interaction 舍曲林和卡培他滨对乳腺癌细胞株的体外联合作用以及硅学中协同作用的证据
IF 2.3 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-15 DOI: 10.3390/scipharm92030038
Serap Ozkaya Gul, Alaaddin Korkut, Esra Aydemir
Background: Depression is a common mood disorder that manifests itself simultaneously with chronic diseases. It is especially common in patients diagnosed with cancer, and when neglected, it reduces the success of cancer treatment. The fact that breast cancer is the most common type of cancer in women shows that the treatment of depression in women with cancer is very important. As a result, cancer patients undergoing chemotherapy in oncology units also use antidepressants simultaneously. It is critical to correctly understand the interactions between drugs used in combination. Method: In this study, doses were prepared for MCF7 and MDAMB-231 cell lines by serial dilution from 1000 ng/mL to 1.95 ng/mL. Cell viability was calculated with the WST-1 kit by applying the prepared doses of capecitabine and sertraline alone. In the sertraline/capecitabine combination study, cell viability was examined in MDAMB-231 and MCF-7 cells by applying doses of 300, 100, 50, 25, 10 ng/mL. Combinations that showed selective cytotoxicity after the combination were analyzed with the CompuSyn program and the combination index (CI<1 = synergism) was calculated. Studies on caspase 3-8-9, DNA fragmentation and mTOR were continued using a combination that showed a synergistic effect. Result: It was determined that compared to drug use alone, the sertraline/capecitabine combination decreased cell viability. There is no significant difference in caspase-3,-8,-9 and DNA fragmentation in cancer cells, but there is a reduction in the level of mTOR. This suggests that the death mechanism may be autophagy. Docking studies with autophagy pathway-related proteins further support our results. It is noteworthy that the AKT1-sertraline complex had the best binding affinity among the target proteins (−9.1 kcal/mol).
背景介绍抑郁症是一种常见的情绪障碍,与慢性疾病同时出现。它在确诊为癌症的患者中尤为常见,一旦被忽视,就会降低癌症治疗的成功率。乳腺癌是女性最常见的癌症类型,这表明治疗女性癌症患者的抑郁症非常重要。因此,在肿瘤科接受化疗的癌症患者也会同时使用抗抑郁药。正确理解联合用药之间的相互作用至关重要。研究方法在本研究中,通过从 1000 纳克/毫升到 1.95 纳克/毫升的系列稀释,为 MCF7 和 MDAMB-231 细胞系制备了剂量。使用 WST-1 试剂盒计算单独使用卡培他滨和舍曲林的细胞活力。在舍曲林/卡培他滨组合研究中,通过使用 300、100、50、25、10 纳克/毫升的剂量检测了 MDAMB-231 和 MCF-7 细胞的细胞活力。使用 CompuSyn 程序分析了组合后显示出选择性细胞毒性的组合,并计算了组合指数(CI<1 = 协同作用)。使用显示出协同效应的组合继续对 Caspase 3-8-9、DNA 断裂和 mTOR 进行研究。研究结果结果表明,与单独用药相比,舍曲林/卡培他滨联合用药会降低细胞活力。癌细胞中的 Caspase-3、-8、-9 和 DNA 断裂没有明显差异,但 mTOR 的水平有所下降。这表明死亡机制可能是自噬。与自噬途径相关蛋白的对接研究进一步支持了我们的结果。值得注意的是,AKT1-舍曲林复合物与目标蛋白的结合亲和力最好(-9.1 kcal/mol)。
{"title":"Combined Effect of Sertraline and Capecitabine on Breast Cancer Cell Lines In Vitro and In Silico Evidence for Synergistic Interaction","authors":"Serap Ozkaya Gul, Alaaddin Korkut, Esra Aydemir","doi":"10.3390/scipharm92030038","DOIUrl":"https://doi.org/10.3390/scipharm92030038","url":null,"abstract":"Background: Depression is a common mood disorder that manifests itself simultaneously with chronic diseases. It is especially common in patients diagnosed with cancer, and when neglected, it reduces the success of cancer treatment. The fact that breast cancer is the most common type of cancer in women shows that the treatment of depression in women with cancer is very important. As a result, cancer patients undergoing chemotherapy in oncology units also use antidepressants simultaneously. It is critical to correctly understand the interactions between drugs used in combination. Method: In this study, doses were prepared for MCF7 and MDAMB-231 cell lines by serial dilution from 1000 ng/mL to 1.95 ng/mL. Cell viability was calculated with the WST-1 kit by applying the prepared doses of capecitabine and sertraline alone. In the sertraline/capecitabine combination study, cell viability was examined in MDAMB-231 and MCF-7 cells by applying doses of 300, 100, 50, 25, 10 ng/mL. Combinations that showed selective cytotoxicity after the combination were analyzed with the CompuSyn program and the combination index (CI<1 = synergism) was calculated. Studies on caspase 3-8-9, DNA fragmentation and mTOR were continued using a combination that showed a synergistic effect. Result: It was determined that compared to drug use alone, the sertraline/capecitabine combination decreased cell viability. There is no significant difference in caspase-3,-8,-9 and DNA fragmentation in cancer cells, but there is a reduction in the level of mTOR. This suggests that the death mechanism may be autophagy. Docking studies with autophagy pathway-related proteins further support our results. It is noteworthy that the AKT1-sertraline complex had the best binding affinity among the target proteins (−9.1 kcal/mol).","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141648301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intravenous Lipid Emulsions in Anticonvulsants’ Toxicity 抗惊厥药物毒性中的静脉注射脂质乳剂
IF 2.3 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-04 DOI: 10.3390/scipharm92030037
S. Dimitrova, S. Dragomanova, G. Kehayova
In recent years, an innovative approach has emerged in the field of toxicology for managing acute intoxications caused by lipophilic substances: intravenous lipid emulsions (ILEs). Through numerous experiments and case reports, the efficacy of lipid emulsions in counteracting toxicities induced by lipophilic agents, including a significant number of antiepileptic (AE) drugs, have become increasingly evident. Data spanning a 10-year period (2010–2020) were analyzed by searching through multiple scientific publication platforms like PubMed, Science Direct, Research Gate, and Springer Link. This study focused on reviewing relevant case reports detailing successful intravenous lipid emulsion (ILE) administration in patients with acute intoxications with antiepileptics, specifically examining the impact of fat emulsions on neurological status, Glasgow Coma Scale (GCS) scores, and corrected QT interval concerning hemodynamic instability. The typical symptoms of antiepileptic toxicity include central nervous system depression, ataxia, and nystagmus. Intravenous lipid emulsion application resulted in an increase in Glasgow Coma Scale scores and enhanced recovery from drug intoxication. This study provides a comprehensive overview of the potential utility of ILE as a component to antidote therapy in cases of acute AE poisoning involving neurotropic drugs. The process involves the engagement of various mechanisms of antitoxic activity.
近年来,毒理学领域出现了一种创新方法来治疗亲脂性物质引起的急性中毒:静脉注射脂质乳剂(ILEs)。通过大量实验和病例报告,脂质乳剂在对抗亲脂性药物(包括大量抗癫痫(AE)药物)引起的中毒方面的功效日益明显。本研究通过搜索 PubMed、Science Direct、Research Gate 和 Springer Link 等多个科学出版物平台,分析了 10 年间(2010-2020 年)的数据。本研究重点回顾了相关病例报告,这些报告详细介绍了抗癫痫药物急性中毒患者成功静脉注射脂质乳剂(ILE)的情况,特别考察了脂质乳剂对神经系统状态、格拉斯哥昏迷量表(GCS)评分以及有关血液动力学不稳定的校正 QT 间期的影响。抗癫痫药物中毒的典型症状包括中枢神经系统抑制、共济失调和眼球震颤。静脉注射脂质乳剂可提高格拉斯哥昏迷量表评分,促进药物中毒的恢复。这项研究全面概述了 ILE 作为解毒剂治疗急性 AE 中毒(涉及神经刺激性药物)病例的潜在作用。这一过程涉及多种解毒机制。
{"title":"Intravenous Lipid Emulsions in Anticonvulsants’ Toxicity","authors":"S. Dimitrova, S. Dragomanova, G. Kehayova","doi":"10.3390/scipharm92030037","DOIUrl":"https://doi.org/10.3390/scipharm92030037","url":null,"abstract":"In recent years, an innovative approach has emerged in the field of toxicology for managing acute intoxications caused by lipophilic substances: intravenous lipid emulsions (ILEs). Through numerous experiments and case reports, the efficacy of lipid emulsions in counteracting toxicities induced by lipophilic agents, including a significant number of antiepileptic (AE) drugs, have become increasingly evident. Data spanning a 10-year period (2010–2020) were analyzed by searching through multiple scientific publication platforms like PubMed, Science Direct, Research Gate, and Springer Link. This study focused on reviewing relevant case reports detailing successful intravenous lipid emulsion (ILE) administration in patients with acute intoxications with antiepileptics, specifically examining the impact of fat emulsions on neurological status, Glasgow Coma Scale (GCS) scores, and corrected QT interval concerning hemodynamic instability. The typical symptoms of antiepileptic toxicity include central nervous system depression, ataxia, and nystagmus. Intravenous lipid emulsion application resulted in an increase in Glasgow Coma Scale scores and enhanced recovery from drug intoxication. This study provides a comprehensive overview of the potential utility of ILE as a component to antidote therapy in cases of acute AE poisoning involving neurotropic drugs. The process involves the engagement of various mechanisms of antitoxic activity.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141678979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence of Hyperglycemic Levels Improving the Binding Capacity between Human Serum Albumin and the Antihypertensive Drug Hydrochlorothiazide 高血糖水平提高人血清白蛋白与抗高血压药物氢氯噻嗪之间结合能力的证据
IF 2.5 Q2 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2024-06-07 DOI: 10.3390/scipharm92020032
Marilia Amável Gomes Soares, F. Souza-Silva, Carlos Roberto Alves, Leonardo Vazquez, T. S. de Araújo, Carlos Serpa, O. A. Chaves
Cardiovascular diseases (CVDs), especially arterial hypertension, stand as prominent contributors to global mortality. Regrettably, individuals with diabetes encounter a two-fold increase in the risk of mortality associated with CVDs. Hydrochlorothiazide (HCTZ) represents a primary intervention for hypertension, particularly in diabetic patients. Nevertheless, there has not yet been a comprehensive assessment of the biophysical characteristics regarding the impact of glucose levels on its binding affinity with human serum albumin (HSA). Thus, the present work reports the interactive profile of HSA/HCTZ in nonglycemic, normoglycemic (80 mg/dL), and hyperglycemic (320 mg/dL) conditions by time-resolved fluorescence, saturation transfer difference–nuclear magnetic resonance (STD-NMR), and surface plasmon resonance (SPR). There was a moderate ground state association of HSA/HCTZ with subdomain IIA that was affected in the presence of different glucose levels. The hyperglycemic condition decreased the binding affinity of HCTZ to subdomain IIA and increased the possibility of subdomain IB also being considered as a secondary binding site due to cooperativity and/or alterations in the protein’s structure. Overall, the glucose level under hyperglycemic conditions led to the cavities being more likely to receive more ligands, offering insights into the necessity of glucose control in the human bloodstream to not impact the residence time (pharmacokinetic profile) and pharmacotherapeutic potential of HCTZ.
心血管疾病(CVD),尤其是动脉高血压,是造成全球死亡的主要原因。令人遗憾的是,糖尿病患者与心血管疾病相关的死亡风险增加了两倍。氢氯噻嗪(HCTZ)是治疗高血压,尤其是糖尿病患者高血压的主要干预药物。然而,关于葡萄糖水平对氢氯噻嗪与人血清白蛋白(HSA)结合亲和力影响的生物物理特性,目前还没有全面的评估。因此,本研究通过时间分辨荧光、饱和转移差核磁共振(STD-NMR)和表面等离子体共振(SPR),报告了 HSA/HCTZ 在非血糖、正常血糖(80 毫克/分升)和高血糖(320 毫克/分升)条件下的相互作用概况。HSA/HCTZ 与亚域 IIA 存在适度的基态关联,这种关联在不同的葡萄糖水平下都会受到影响。高血糖条件降低了 HCTZ 与亚域 IIA 的结合亲和力,并增加了亚域 IB 因协同作用和/或蛋白质结构的改变而被视为次级结合位点的可能性。总之,高血糖条件下的葡萄糖水平导致空腔更有可能接受更多配体,这使人们了解到人体血液中葡萄糖控制的必要性,从而不影响 HCTZ 的停留时间(药动学特征)和药理治疗潜力。
{"title":"Evidence of Hyperglycemic Levels Improving the Binding Capacity between Human Serum Albumin and the Antihypertensive Drug Hydrochlorothiazide","authors":"Marilia Amável Gomes Soares, F. Souza-Silva, Carlos Roberto Alves, Leonardo Vazquez, T. S. de Araújo, Carlos Serpa, O. A. Chaves","doi":"10.3390/scipharm92020032","DOIUrl":"https://doi.org/10.3390/scipharm92020032","url":null,"abstract":"Cardiovascular diseases (CVDs), especially arterial hypertension, stand as prominent contributors to global mortality. Regrettably, individuals with diabetes encounter a two-fold increase in the risk of mortality associated with CVDs. Hydrochlorothiazide (HCTZ) represents a primary intervention for hypertension, particularly in diabetic patients. Nevertheless, there has not yet been a comprehensive assessment of the biophysical characteristics regarding the impact of glucose levels on its binding affinity with human serum albumin (HSA). Thus, the present work reports the interactive profile of HSA/HCTZ in nonglycemic, normoglycemic (80 mg/dL), and hyperglycemic (320 mg/dL) conditions by time-resolved fluorescence, saturation transfer difference–nuclear magnetic resonance (STD-NMR), and surface plasmon resonance (SPR). There was a moderate ground state association of HSA/HCTZ with subdomain IIA that was affected in the presence of different glucose levels. The hyperglycemic condition decreased the binding affinity of HCTZ to subdomain IIA and increased the possibility of subdomain IB also being considered as a secondary binding site due to cooperativity and/or alterations in the protein’s structure. Overall, the glucose level under hyperglycemic conditions led to the cavities being more likely to receive more ligands, offering insights into the necessity of glucose control in the human bloodstream to not impact the residence time (pharmacokinetic profile) and pharmacotherapeutic potential of HCTZ.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141373788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Medicinal Use of Chicory (Cichorium intybus L.) 菊苣(Cichorium intybus L.)的药用价值
IF 2.5 Q2 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2024-06-05 DOI: 10.3390/scipharm92020031
Łukasz Duda, K. Kłosiński, G. Budryn, Andrzej Jaśkiewicz, D. Kołat, Ż. Kałuzińska-Kołat, Zbigniew Pasieka
The aim of this review is to discuss the numerous health-promoting properties of Cichorium intybus L. and bring together a range of publications to broaden knowledge and encourage further research and consideration of the plant use as treatment for a range of conditions. A comprehensive search of articles in Polish and English from 1986–2022 years was carried out in PubMed, Google Scholar and ScienceDirect using the keywords chicory, Cichorium intybus L., sesquiterpene lactones and their synonyms. Articles were checked for titles, abstracts, and full-text reviews. The first part of the review article discusses chicory, the countries in which it is found, its life cycle or modern cultivation methods, as well as its many uses, which will be discussed in more detail later in the article. The increased interest in plants as medicines or supplements is also briefly mentioned, as well as some limits that are associated with the medical use of plants. In the Results and Discussion section, there is a discussion of the numerous health-promoting properties of Cichorium intybus L. as a whole plant, with its collection of all the components, and we then examine the structure and the individual constituents of Cichorium intybus L. Among these, this article discusses those that can be utilized for causal applications in medicine, including sesquiterpene lactones and polyphenols, mainly known for their anti-cancer properties, although, in this article, their other health-promoting properties are also discussed. The article also examines inulin, a major component of Cichorium intybus L. The Discussion and the Conclusions sections propose directions for more detailed research and the range of factors that may affect specific results, which may have safety implications when used as supplements or medications.
本综述旨在讨论 Cichorium intybus L. 的众多健康促进特性,并汇集一系列出版物,以拓宽知识面,鼓励进一步研究和考虑使用该植物治疗各种疾病。在 PubMed、Google Scholar 和 ScienceDirect 上以菊苣、Cichorium intybus L.、倍半萜内酯及其同义词为关键词对 1986-2022 年间的波兰语和英语文章进行了全面搜索。对文章的标题、摘要和综述全文进行了检查。综述文章的第一部分讨论了菊苣、菊苣分布的国家、菊苣的生命周期或现代栽培方法,以及菊苣的多种用途,这些将在文章的后面部分详细讨论。此外,还简要提到了人们对植物作为药物或补充剂的兴趣日益浓厚,以及与植物医疗用途相关的一些限制。在 "结果与讨论 "部分,我们讨论了 Cichorium intybus L.作为一个整体植物所具有的众多促进健康的特性,以及它所收集的所有成分,然后我们研究了 Cichorium intybus L.的结构和单个成分,其中,本文讨论了可用于医学的因果应用的成分,包括倍半萜内酯和多酚,它们主要以抗癌特性而闻名,但本文也讨论了它们的其他促进健康的特性。文章还研究了菊粉(Cichorium intybus L.的一种主要成分)。"讨论 "和 "结论 "部分提出了更详细的研究方向,以及可能影响具体结果的各种因素,这些因素在用作补充剂或药物时可能会产生安全影响。
{"title":"Medicinal Use of Chicory (Cichorium intybus L.)","authors":"Łukasz Duda, K. Kłosiński, G. Budryn, Andrzej Jaśkiewicz, D. Kołat, Ż. Kałuzińska-Kołat, Zbigniew Pasieka","doi":"10.3390/scipharm92020031","DOIUrl":"https://doi.org/10.3390/scipharm92020031","url":null,"abstract":"The aim of this review is to discuss the numerous health-promoting properties of Cichorium intybus L. and bring together a range of publications to broaden knowledge and encourage further research and consideration of the plant use as treatment for a range of conditions. A comprehensive search of articles in Polish and English from 1986–2022 years was carried out in PubMed, Google Scholar and ScienceDirect using the keywords chicory, Cichorium intybus L., sesquiterpene lactones and their synonyms. Articles were checked for titles, abstracts, and full-text reviews. The first part of the review article discusses chicory, the countries in which it is found, its life cycle or modern cultivation methods, as well as its many uses, which will be discussed in more detail later in the article. The increased interest in plants as medicines or supplements is also briefly mentioned, as well as some limits that are associated with the medical use of plants. In the Results and Discussion section, there is a discussion of the numerous health-promoting properties of Cichorium intybus L. as a whole plant, with its collection of all the components, and we then examine the structure and the individual constituents of Cichorium intybus L. Among these, this article discusses those that can be utilized for causal applications in medicine, including sesquiterpene lactones and polyphenols, mainly known for their anti-cancer properties, although, in this article, their other health-promoting properties are also discussed. The article also examines inulin, a major component of Cichorium intybus L. The Discussion and the Conclusions sections propose directions for more detailed research and the range of factors that may affect specific results, which may have safety implications when used as supplements or medications.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141382991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exposure to Benzo(a)pyrene Enhances Acetaminophen-Induced Liver Injury in Mice at Non-Hepatotoxic Doses 接触苯并(a)芘会增强对乙酰氨基酚诱发小鼠肝损伤的非肝毒性剂量
IF 2.5 Q2 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2024-06-03 DOI: 10.3390/scipharm92020030
Yina Montero-Pérez, J. Olivero-Verbel
Acetaminophen (APAP) is a widely used analgesic, especially for children. Its primary mechanism involves inhibiting cyclooxygenase enzymes and activating the endocannabinoid and TRPV1 systems. Though its toxicity is low, it can harm the liver in a dose-dependent manner. Low APAP doses can also increase pollutant-induced liver damage. Little is known about interactions between APAP and benzo[a]pyrene (B[a]P). This study aimed to assess if co-exposure to non-hepatotoxic doses of B[a]P and APAP causes liver injury in mice, exploring the underlying mechanisms. Female ICR mice received 50 mg/kg B[a]P or a vehicle for three days, followed by 200 mg/kg APAP or a vehicle. Liver injury was assessed through histopathological examination, serum transaminase activity, and gene expression analysis. In the B[a]P/APAP group, several histology changes were observed, including ballooning injury, steatosis, necrosis, inflammation, and apoptosis. Transaminase levels correlated with histopathological scores, and there was an increase in hepatic cytochrome P450 family 1 subfamily a member 1 (Cyp1a1) mRNA levels and a decrease in aryl hydrocarbon receptor (Ahr), cytochrome P450 family 2 subfamily e polypeptide 1 (Cyp2e1), superoxide dismutase 1 (Sod1), peroxisome proliferator activated receptor gamma (Ppar-γ), and caspase 3 (Casp3). This suggests that prior exposure to B[a]P makes mice more susceptible to APAP-induced liver injury, involving changes in gene expression related to metabolism, redox balance, and cell proliferation. Therefore, using therapeutic APAP doses after exposure to B[a]P could lead to liver injury.
对乙酰氨基酚(APAP)是一种广泛使用的镇痛药,尤其适用于儿童。其主要机制是抑制环氧化酶,激活内源性大麻素和 TRPV1 系统。虽然其毒性较低,但会以剂量依赖的方式损害肝脏。低剂量的 APAP 也会增加污染物引起的肝损伤。人们对 APAP 与苯并[a]芘(B[a]P)之间的相互作用知之甚少。本研究旨在评估同时暴露于非肝毒性剂量的苯并[a]芘和 APAP 是否会导致小鼠肝损伤,并探索其潜在机制。雌性 ICR 小鼠接受 50 毫克/千克 B[a]P 或载体治疗三天,然后再接受 200 毫克/千克 APAP 或载体治疗三天。肝损伤通过组织病理学检查、血清转氨酶活性和基因表达分析进行评估。在B[a]P/APAP组中,观察到了几种组织学变化,包括气球损伤、脂肪变性、坏死、炎症和细胞凋亡。转氨酶水平与组织病理学评分相关,肝细胞色素 P450 家族 1 亚家族 a 成员 1(Cyp1a1)mRNA 水平升高,芳基烃受体(Ahr)、细胞色素 P450 家族 2 亚家族 e 多肽 1(Cyp2e1)、超氧化物歧化酶 1(Sod1)、过氧化物酶体增殖激活受体 gamma(Ppar-γ)和 Caspase 3(Casp3)水平降低。这表明,事先暴露于 B[a]P 会使小鼠更容易受到 APAP 诱导的肝损伤的影响,其中涉及与新陈代谢、氧化还原平衡和细胞增殖有关的基因表达变化。因此,在暴露于 B[a]P 后使用治疗剂量的 APAP 可能会导致肝损伤。
{"title":"Exposure to Benzo(a)pyrene Enhances Acetaminophen-Induced Liver Injury in Mice at Non-Hepatotoxic Doses","authors":"Yina Montero-Pérez, J. Olivero-Verbel","doi":"10.3390/scipharm92020030","DOIUrl":"https://doi.org/10.3390/scipharm92020030","url":null,"abstract":"Acetaminophen (APAP) is a widely used analgesic, especially for children. Its primary mechanism involves inhibiting cyclooxygenase enzymes and activating the endocannabinoid and TRPV1 systems. Though its toxicity is low, it can harm the liver in a dose-dependent manner. Low APAP doses can also increase pollutant-induced liver damage. Little is known about interactions between APAP and benzo[a]pyrene (B[a]P). This study aimed to assess if co-exposure to non-hepatotoxic doses of B[a]P and APAP causes liver injury in mice, exploring the underlying mechanisms. Female ICR mice received 50 mg/kg B[a]P or a vehicle for three days, followed by 200 mg/kg APAP or a vehicle. Liver injury was assessed through histopathological examination, serum transaminase activity, and gene expression analysis. In the B[a]P/APAP group, several histology changes were observed, including ballooning injury, steatosis, necrosis, inflammation, and apoptosis. Transaminase levels correlated with histopathological scores, and there was an increase in hepatic cytochrome P450 family 1 subfamily a member 1 (Cyp1a1) mRNA levels and a decrease in aryl hydrocarbon receptor (Ahr), cytochrome P450 family 2 subfamily e polypeptide 1 (Cyp2e1), superoxide dismutase 1 (Sod1), peroxisome proliferator activated receptor gamma (Ppar-γ), and caspase 3 (Casp3). This suggests that prior exposure to B[a]P makes mice more susceptible to APAP-induced liver injury, involving changes in gene expression related to metabolism, redox balance, and cell proliferation. Therefore, using therapeutic APAP doses after exposure to B[a]P could lead to liver injury.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141271268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Drug Delivery Nano-Platforms for Advanced Cancer Therapy 用于先进癌症治疗的纳米给药平台
IF 2.5 Q2 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2024-05-22 DOI: 10.3390/scipharm92020028
Ekaterina Naumenko, Ivan Guryanov, M. Gomzikova
The incidence of cancer is growing every year and covers all age groups, including the working population, which makes cancer socially significant. Existing methods of treatment, despite the effectiveness of individual compounds in relation to cancer cells, are not perfect due to a number of side effects associated with high doses that physicians are forced to administer when using treatment protocols. A particularly difficult issue is the creation of effective functional containers that would have the properties of targeting certain types of cells. The solution of this problem is currently relevant, which is reflected in the growth of publications on this subject in recent years. The most promising is the use of nanotechnology in the development of bioengineered therapeutics and containers for chemotherapeutic agents. In this review, we tried to assess the trends that exist in this area of research, as well as show the wide using of some commercially available formulations based on the nano-sized vehicles.
癌症的发病率每年都在增长,覆盖了所有年龄段,包括工作人群,这使得癌症具有重要的社会意义。尽管个别化合物对癌细胞有效,但现有的治疗方法并不完美,因为医生在使用治疗方案时不得不使用高剂量,从而产生了许多副作用。一个特别困难的问题是,如何创造出有效的功能性容器,使其具有靶向特定类型细胞的特性。目前,这一问题的解决具有现实意义,这体现在近年来有关这一主题的出版物不断增加。最有前景的是利用纳米技术开发生物工程治疗剂和化疗剂容器。在这篇综述中,我们试图评估这一研究领域的发展趋势,并展示一些基于纳米级载体的商用制剂的广泛应用。
{"title":"Drug Delivery Nano-Platforms for Advanced Cancer Therapy","authors":"Ekaterina Naumenko, Ivan Guryanov, M. Gomzikova","doi":"10.3390/scipharm92020028","DOIUrl":"https://doi.org/10.3390/scipharm92020028","url":null,"abstract":"The incidence of cancer is growing every year and covers all age groups, including the working population, which makes cancer socially significant. Existing methods of treatment, despite the effectiveness of individual compounds in relation to cancer cells, are not perfect due to a number of side effects associated with high doses that physicians are forced to administer when using treatment protocols. A particularly difficult issue is the creation of effective functional containers that would have the properties of targeting certain types of cells. The solution of this problem is currently relevant, which is reflected in the growth of publications on this subject in recent years. The most promising is the use of nanotechnology in the development of bioengineered therapeutics and containers for chemotherapeutic agents. In this review, we tried to assess the trends that exist in this area of research, as well as show the wide using of some commercially available formulations based on the nano-sized vehicles.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141109439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioactive Components Analysis and Pharmacological Properties of Extracts and Metabolites of Lichen Umbilicaria crustulosa 地衣中提取物和代谢物的生物活性成分分析及药理特性
IF 2.5 Q2 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2024-05-20 DOI: 10.3390/scipharm92020027
J. Tomović, Aleksandar Kočović, Marijana Anđić, Jovana V Bradic, Nevena Zubić, V. Jakovljević, Miroslav Sovrlić, P. Vasiljević, N. Manojlovic
Lichens, a diverse group of organisms, have a unique structure consisting of fungal filaments and photosynthetic partner cells. This research conducted a comprehensive chemical analysis and evaluation of the anti-inflammatory and antioxidant properties of methanolic and acetone extracts from Umbilicaria crustulosa lichen, along with its isolated metabolites. The process involved separating atranorin and chloratranorin fractions, physodic acid, and gyrophoric acid. Secondary metabolites were identified using chromatographic and spectroscopic data. The total polyphenols content was determined spectrophotometrically. This study examined the antioxidant activity of extracts of the lichen U. crustulosa and the isolated fractions using three methods: DPPH scavenging activity, ABTS scavenging activity, and reducing power. This study also evaluated the acute oral toxicity and the anti-inflammatory activity of the extracts in Wistar albino rats. A higher content of the total phenolic compounds was found in the acetone extract, but antioxidant and anti-inflammatory activities were more prominent in the methanolic extract. The isolated atranorin and chloratranorin fractions and compound physodic acid showed the highest antioxidant activity. No toxic effects were noted in the acute oral toxicity study. This study highlights the potential of the investigated lichen as a valuable source of novel biological agents.
地衣是一种多种多样的生物,具有由真菌菌丝和光合作用伴侣细胞组成的独特结构。这项研究对甲醇和丙酮提取物进行了全面的化学分析,并评估了甲壳鞘氨醇地衣及其分离代谢物的抗炎和抗氧化特性。这一过程包括分离阿曲霉素和氯曲霉素馏分、物理酸和石膏酸。利用色谱和光谱数据对次生代谢物进行了鉴定。总多酚含量采用分光光度法测定。本研究采用三种方法检测了地衣 U. crustulosa 提取物和分离馏分的抗氧化活性:DPPH 清除活性、ABTS 清除活性和还原力。该研究还评估了提取物对 Wistar 白化大鼠的急性口服毒性和抗炎活性。丙酮提取物中总酚类化合物的含量较高,但甲醇提取物的抗氧化和抗炎活性更为突出。分离出的阿曲霉苷和氯曲霉苷馏分以及复合物理酸显示出最高的抗氧化活性。在急性口服毒性研究中未发现任何毒性作用。这项研究凸显了所研究地衣作为新型生物制剂宝贵来源的潜力。
{"title":"Bioactive Components Analysis and Pharmacological Properties of Extracts and Metabolites of Lichen Umbilicaria crustulosa","authors":"J. Tomović, Aleksandar Kočović, Marijana Anđić, Jovana V Bradic, Nevena Zubić, V. Jakovljević, Miroslav Sovrlić, P. Vasiljević, N. Manojlovic","doi":"10.3390/scipharm92020027","DOIUrl":"https://doi.org/10.3390/scipharm92020027","url":null,"abstract":"Lichens, a diverse group of organisms, have a unique structure consisting of fungal filaments and photosynthetic partner cells. This research conducted a comprehensive chemical analysis and evaluation of the anti-inflammatory and antioxidant properties of methanolic and acetone extracts from Umbilicaria crustulosa lichen, along with its isolated metabolites. The process involved separating atranorin and chloratranorin fractions, physodic acid, and gyrophoric acid. Secondary metabolites were identified using chromatographic and spectroscopic data. The total polyphenols content was determined spectrophotometrically. This study examined the antioxidant activity of extracts of the lichen U. crustulosa and the isolated fractions using three methods: DPPH scavenging activity, ABTS scavenging activity, and reducing power. This study also evaluated the acute oral toxicity and the anti-inflammatory activity of the extracts in Wistar albino rats. A higher content of the total phenolic compounds was found in the acetone extract, but antioxidant and anti-inflammatory activities were more prominent in the methanolic extract. The isolated atranorin and chloratranorin fractions and compound physodic acid showed the highest antioxidant activity. No toxic effects were noted in the acute oral toxicity study. This study highlights the potential of the investigated lichen as a valuable source of novel biological agents.","PeriodicalId":21601,"journal":{"name":"Scientia Pharmaceutica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141122764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Scientia Pharmaceutica
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1