Galactose oxidase/kelch repeat-containing protein is involved in the iron deficiency stress response in the roots of Hyoscyamus albus

IF 1 Q3 PLANT SCIENCES Plant Root Pub Date : 2017-11-30 DOI:10.3117/PLANTROOT.11.58
Yuki Kawahara, T. Hashimoto, H. Nakayama, Y. Kitamura
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引用次数: 6

Abstract

To confirm the involvement of galactose oxidase/kelch repeat-containing protein (Glx) in response to iron (Fe) deficiency in Hyoscyamus albus, we cloned a putative full-length HaGlx cDNA, which contained an open reading frame (975 bp, 324 amino acids). HaGlx was confirmed by homology searches, molecular phylogeny analysis, and domain search. HaGlx was expressed in the roots but not in the leaves, and the expression significantly increased under Fe deficiency. Sequencing of ~1.9 kb of the 5ʹ-upstream region of the HaGlx gene, followed by the analysis of promoter elements, resulted in the identification of multiple rootspecific elements together with stress-induced elements, including the Fe deficiency-induced element (IDE1) core motif. This suggests that HaGlx plays a key role in stress responses induced under Fe deficiency in the roots. To our knowledge, this is the first report confirming, in a plant other than Arabidopsis thaliana, that Glx is involved in the stress response to Fe deficiency.
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半乳糖氧化酶/含kelch重复蛋白参与了水仙根系缺铁胁迫反应
为了证实半乳糖氧化酶/kelch重复序列蛋白(Glx)参与了对黄杨铁(Fe)缺乏的反应,我们克隆了一个假定的全长HaGlx cDNA,该cDNA包含一个开放阅读框(975bp,324个氨基酸)。通过同源性搜索、分子系统发育分析和结构域搜索证实了HaGlx。HaGlx在根中表达,但在叶中不表达,并且在Fe缺乏的情况下表达显著增加。对HaGlx基因5上游区约1.9 kb进行测序,然后分析启动子元件,从而鉴定出多个根特异性元件和胁迫诱导元件,包括缺铁诱导元件(IDE1)核心基序。这表明HaGlx在根中Fe缺乏诱导的应激反应中起着关键作用。据我们所知,这是第一份在拟南芥以外的植物中证实Glx参与铁缺乏的应激反应的报告。
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来源期刊
Plant Root
Plant Root PLANT SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
期刊介绍: Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.
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