Comparative study of radio-sensitivity and relative biological effectiveness of gamma rays, x-rays, electron beam and proton beam in short grain aromatic rice

IF 1 4区 生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2020-11-30 DOI:10.31742/ijgpb.80.4.3
Deepak Kumar Sharma, Richa Sao, P. Sahu, G. Vishwakarma, J. P. Nair, V. Petwal, B. K. Das
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引用次数: 7

Abstract

Knowledge about the type of mutagen used and its optimized dose are of paramount importance to design and implement any plant mutation breeding programme. Present study was first time carried out to evaluate the comparative effectiveness, radio-sensitivity behavior and relative biological effectiveness of four physical mutagens viz., gamma rays, X-rays, electron beam and proton beam on two short grain aromatic rice landraces viz., Samundchini and Vishnubhog. The seeds of these two varieties were treated with 15 different doses of all four mutagens, ranging from 50Gy to 750Gy with an interval of 50Gy. Germination percentage and seedling growth parameters were recorded at seven and 15 days after sowing, respectively in two replications. It was observed that germination percentage, shoot and root length of the seedling gradually declined with the increase in doses of all the physical mutagens. On the basis of these observations, LD50 and GR50 doses were calculated. The present study reports the optimum range of doses for gamma ray (280 to 350 Gy); electron beam (290 to 330Gy); X-ray (200 to 250 Gy) and proton beam (150 to 200Gy). GR50 doses were observed higher than LD50 doses for all the mutagens in both landraces. However, Samundchini showed higher LD50 and GR50 doses than Vishnubhog indicating later to be more radio-sensitive. Furthermore, both the genotypes were highly radio-sensitive for proton beam and least for gamma rays. Similarly, high relative biological effectiveness was observed for proton beam followed by X-ray, electron beam and gamma rays indicating their decreasing trend of penetration capacity and lethality. Results of present study will be useful for plant breeders to use the above mutagens in an appropriate dose for mutation breeding in rice.
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短粒香稻γ射线、x射线、电子束和质子束辐射敏感性及相对生物效应的比较研究
了解所使用的诱变剂类型及其最佳剂量对于设计和实施任何植物诱变育种计划至关重要。本研究首次对四种物理诱变剂(伽马射线、x射线、电子束和质子束)对两种短粒香稻地方品种Samundchini和Vishnubhog的相对诱变效果、辐射敏感性行为和相对生物效应进行了评价。对这两个品种的种子进行了15种不同剂量的诱变剂处理,剂量从50Gy到750Gy不等,间隔为50Gy。分别在播种后7天和15 d记录发芽率和幼苗生长参数。结果表明,随着物理诱变剂剂量的增加,幼苗的发芽率、茎长和根长逐渐下降。在这些观察的基础上,计算了LD50和GR50剂量。本研究报告了伽马射线的最佳剂量范围(280至350戈瑞);电子束(290 ~ 330Gy);x射线(200至250 Gy)和质子束(150至200Gy)。在两个地方品种中,所有诱变剂的GR50剂量均高于LD50剂量。然而,Samundchini显示出比Vishnubhog更高的LD50和GR50剂量,表明后来对放射性更敏感。此外,这两种基因型对质子束的辐射敏感性都很高,对伽马射线的辐射敏感性最低。同样,质子束的相对生物效应较高,其次是x射线、电子束和伽马射线,表明它们的穿透能力和致死率呈下降趋势。本研究的结果将为植物育种者选择适当剂量的诱变剂进行水稻诱变育种提供参考。
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来源期刊
CiteScore
1.80
自引率
10.00%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
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