GC-MS Based Profiling, Antimicrobial Activity and Cytotoxicity Studies of Camellia sinensis (L.) O. Kuntze Flower Extract

Pub Date : 2022-03-04 DOI:10.1080/22311866.2022.2049643

Ranjana Sharma, Smita Kapoor, Y. Padwad, D. Kumar

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引用次数: 2

Abstract

Abstract Camellia sinensis flowers are rich cradle of bioactive phytochemicals yet treated as waste biomass of the bush. However, flowers are used for the treatment of diverse health ailments since ages. Thus solvent free supercritical fluid extract of C. sinensis flowers was targeted to explore the phytochemical and bioactive potential. Parameters for the extraction were optimized at 300 bar pressure, temperature 45°C, static time 15-20 minutes and dynamic time 90 minutes that yield 1.005% (1.005g/100g) extract. The chemical characterization of extract (Gas chromatography-mass spectrometric analysis) resulted in the identification of eighteen non-polar compounds. Furthermore, derivatization (silylation) identified five unsaturated fatty acids and a terpene alcohol. The antimicrobial studies of C. sinensis flowers revealed strong antifungal (20-27 mm) and appreciable antibacterial (7-12 mm) potential at minimum concentrations (1 mg/mL). The cytotoxicity studies of tea flower extract against colorectal cancer cell lines (HCT-116 and HT-29) showed cell death up to 80±0.80% that highlighted the importance of economically underutilized tea flowers. This also revealed wide scope for their utilization in pharmaceutical, nutraceutical and cosmetic sectors. GRAPHICAL ABSTRACT

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山茶花提取物的GC-MS图谱、抗菌活性及细胞毒性研究

摘要山茶花是富含生物活性植物化学物质的摇篮，但却被视为灌木的废弃生物。然而，花自古以来就被用于治疗各种健康疾病。因此，以无溶剂超临界流体提取物为目标，探索其植物化学和生物活性潜力。在300巴的压力、45°C的温度、15-20分钟的静态时间和90分钟的动态时间下优化提取参数，得到1.005%（1.005g/100g）的提取物。提取物的化学特征（气相色谱-质谱分析）鉴定出18种非极性化合物。此外，衍生（甲硅烷基化）鉴定出五种不饱和脂肪酸和一种萜烯醇。对中华鳖花的抗菌研究表明，在最低浓度（1 mg/mL）下，中华鳖花具有较强的抗真菌能力（20-27 mm）和可观的抗菌潜力（7-12 mm）。茶叶提取物对结直肠癌癌症细胞系（HCT-116和HT-29）的细胞毒性研究表明，细胞死亡高达80±0.80%，这突出了经济上未充分利用的茶叶的重要性。这也揭示了它们在制药、营养品和化妆品行业的广泛应用范围。图形摘要

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