Effects of scutellarin on acute myocardial ischemia/reperfusion injury in isolated rat heart

IF 0.6 4区 医学 Q4 IMMUNOLOGY European Journal of Inflammation Pub Date : 2023-08-07 DOI:10.1177/1721727x231192289
Daoxu Qu, Peiwen Feng, Xinjie Zhang, Mingjie Zhou
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Abstract

This study aimed to evaluate the cardioprotective effects and mechanism of scutellarin (Scu) on heart ischaemia/reperfusion (I/R) injury by using an isolated Langendorff rat heart model. Myocardial function was evaluated via measurements of cardiac haemodynamic parameters. Myocardial infarct size was macroscopically estimated by using TTC staining. The release of creatine kinase (CK) and lactate dehydrogenase (LDH) was used to evaluate cardiac injury. GSH/GSSG, SOD, and MDA were determined as being indicators of oxidative stress. CRP, IL-6, and TNF-α were also analysed to assess inflammation in the heart tissues. Cardiomyocyte apoptosis was quantified by using a TUNEL kit. PPARγ, Nrf2, and NF-κB were assayed via Western blotting. The results indicated that I/R induced significant cardiac dysfunction, myocardial infarct, and apoptosis, as well as decreasing the GSH/GSSG ratio, SOD activity, Nrf2 protein expression, and PPARγ protein expression, but increased levels of TNF-α, MDA, CRP, IL-6, and NF-κB. These data suggest that Scu attenuates cardiac I/R.
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黄芩苷对离体大鼠急性心肌缺血再灌注损伤的影响
本研究旨在采用Langendorff大鼠离体心脏模型,评价灯盏花素(Scu)对心脏缺血/再灌注(I/R)损伤的保护作用及其机制。通过测量心脏血流动力学参数来评估心肌功能。心肌梗死面积通过TTC染色进行宏观评估。肌酸激酶(CK)和乳酸脱氢酶(LDH)的释放用于评估心脏损伤。GSH/GSSG、SOD和MDA被确定为氧化应激的指标。还分析了CRP、IL-6和TNF-α,以评估心脏组织中的炎症。通过使用TUNEL试剂盒对心肌细胞凋亡进行定量。通过蛋白质印迹法测定PPARγ、Nrf2和NF-κB。结果表明,I/R可诱导显著的心功能障碍、心肌梗死和细胞凋亡,并降低GSH/GSSG比值、SOD活性、Nrf2蛋白表达和PPARγ蛋白表达,但增加TNF-α、MDA、CRP、IL-6和NF-κB的水平。这些数据表明,Scu可减弱心脏I/R。
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
54
审稿时长
15 weeks
期刊介绍: European Journal of Inflammation is a multidisciplinary, peer-reviewed, open access journal covering a wide range of topics in inflammation, including immunology, pathology, pharmacology and related general experimental and clinical research.
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