Micropropagation of Anthurium through suspension culture using in vitro shoots

Abstract

A simpler and more efficient protocol for mass propagation of Anthurium from somatically derived in vitro shoots through suspension culture was developed. Shoot proliferation was obtained with agar-solidified MS medium as well as liquid MS medium, at various concentration of BAP. After 45 days of incubation at 80 rpm, both the media gave best results at 8.8 μM BAP, whereas higher number (29.333±0.342) of shoots was obtained with liquid medium compared to solid medium (7.667±0.342). The matured plantlets were harvested and subjected to acclimatization after 30 days of primary and 60 days of secondary hardening and then they were transferred to field. Survival rate of plants (71.3 %) in liquid medium was higher compared to the ones from solid medium (43.0 %).