Pub Date : 2022-04-25DOI: 10.37855/jah.2022.v24i01.15
H. Hussain, S.I.L. Kamarol, N. Julaihi, R. Tomm
Sago palm (Metroxylon sagu Rottb.) is an economically valuable crop and cultivated widely in Sarawak, Malaysia, for its storage starch. Under certain conditions, such as deep peat areas, some sago palms do not develop well and become stunted, described as non-trunking sago palms. Morphologically, this type of sago palm is abnormal, leading to wastage of various resources and affecting starch production. The research objective was to understand the molecular process that affects non-trunking and starch synthesis in sago palm. Representational Difference Analysis was performed to identify the differences between trunking and non-trunking sago palm transcriptome. This subtractive hybridisation technique detects the differences in gene expression. After three rounds of hybridisation, the differentiated RDA products were cloned and 24 DNA sequences obtained were compared with the sequences in the NCBI database. Results showed that the differentiated genes in response to the non-trunking sago palm have similarities to genes implicated in plant growth, cell metabolism, and salt or osmotic stress. Some of these genes are related to growth, development and signaling involving neutralising the imbalance in plant stress. The information obtained through this work could be used to develop a database on related genes and enzymes contributing to trunking and non-trunking sago palm.
{"title":"Identification of gene transcripts contributing to trunking and non-trunking sago palm (Metroxylon sagu Rottb.)","authors":"H. Hussain, S.I.L. Kamarol, N. Julaihi, R. Tomm","doi":"10.37855/jah.2022.v24i01.15","DOIUrl":"https://doi.org/10.37855/jah.2022.v24i01.15","url":null,"abstract":"Sago palm (Metroxylon sagu Rottb.) is an economically valuable crop and cultivated widely in Sarawak, Malaysia, for its storage starch. Under certain conditions, such as deep peat areas, some sago palms do not develop well and become stunted, described as non-trunking sago palms. Morphologically, this type of sago palm is abnormal, leading to wastage of various resources and affecting starch production. The research objective was to understand the molecular process that affects non-trunking and starch synthesis in sago palm. Representational Difference Analysis was performed to identify the differences between trunking and non-trunking sago palm transcriptome. This subtractive hybridisation technique detects the differences in gene expression. After three rounds of hybridisation, the differentiated RDA products were cloned and 24 DNA sequences obtained were compared with the sequences in the NCBI database. Results showed that the differentiated genes in response to the non-trunking sago palm have similarities to genes implicated in plant growth, cell metabolism, and salt or osmotic stress. Some of these genes are related to growth, development and signaling involving neutralising the imbalance in plant stress. The information obtained through this work could be used to develop a database on related genes and enzymes contributing to trunking and non-trunking sago palm.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43505516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-25DOI: 10.37855/jah.2021.v23i03.68
N. Verma, Ravi Kumar, J. Kaur, D. S. Thakur
Artificial pollination of vegetable crops necessitates the knowledge of crop's floral biology. In this aspect present investigations were carried out, to gather the information on the floral biology of monoecious (K-75 and UHF-CUC-101) and gynoecious (GYNO-1 and GYNO-2) varieties of cucumber at the Experimental Research Farm, Department of Vegetable Science, Dr. YSPUHF Nauni, Solan (HP) during Kharif, 2016. The experiment was laid out in a RCBD with three replications. The observations were recorded on time of anthesis, dehiscence, pollen viability (%), stigma receptivity and node number bearing first female flower. The experimental results showed that anthesis started at 6AM and completed by 8AM with the maximum anthesis between 6AM to 7AM in monoecious varieties whereas, in gynoecious varieties it was maximum upto 6:00AM in both open and controlled conditions and similar pattern was observed for dehiscence. Dehiscence occurs soon after anthesis. Maximum pollen viability was recorded on the day of anthesis and viability of pollen under refrigerated condition did not decrease as rapidly as it was under room temperature condition with the duration of pollen storage. Maximum stigma receptivity was noticed at anthesis time and pollination during this interval recorded maximum fruit set. Gynoecious lines were earlier in flowering and fruiting than monoecious varieties.
{"title":"Floral biology studies in cucumber (Cucumis sativus L.)","authors":"N. Verma, Ravi Kumar, J. Kaur, D. S. Thakur","doi":"10.37855/jah.2021.v23i03.68","DOIUrl":"https://doi.org/10.37855/jah.2021.v23i03.68","url":null,"abstract":"Artificial pollination of vegetable crops necessitates the knowledge of crop's floral biology. In this aspect present investigations were carried out, to gather the information on the floral biology of monoecious (K-75 and UHF-CUC-101) and gynoecious (GYNO-1 and GYNO-2) varieties of cucumber at the Experimental Research Farm, Department of Vegetable Science, Dr. YSPUHF Nauni, Solan (HP) during Kharif, 2016. The experiment was laid out in a RCBD with three replications. The observations were recorded on time of anthesis, dehiscence, pollen viability (%), stigma receptivity and node number bearing first female flower. The experimental results showed that anthesis started at 6AM and completed by 8AM with the maximum anthesis between 6AM to 7AM in monoecious varieties whereas, in gynoecious varieties it was maximum upto 6:00AM in both open and controlled conditions and similar pattern was observed for dehiscence. Dehiscence occurs soon after anthesis. Maximum pollen viability was recorded on the day of anthesis and viability of pollen under refrigerated condition did not decrease as rapidly as it was under room temperature condition with the duration of pollen storage. Maximum stigma receptivity was noticed at anthesis time and pollination during this interval recorded maximum fruit set. Gynoecious lines were earlier in flowering and fruiting than monoecious varieties.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41781280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2021.v23i02.20
B. Petrović, R. Pokluda, C. Richard, K. Fonteno
{"title":"Production cost analysis of organic and conventional vegetable seedlings","authors":"B. Petrović, R. Pokluda, C. Richard, K. Fonteno","doi":"10.37855/jah.2021.v23i02.20","DOIUrl":"https://doi.org/10.37855/jah.2021.v23i02.20","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43215409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2021.v23i02.32
O. Abba, A. Rochdi
{"title":"Feasibility of using solar energy for cold production","authors":"O. Abba, A. Rochdi","doi":"10.37855/jah.2021.v23i02.32","DOIUrl":"https://doi.org/10.37855/jah.2021.v23i02.32","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43524991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2020.v22i03.49
Y. Setiyo, I. Gunam, B. A. Harsojuwono, K. A. Yuliadhi
{"title":"Analysis of the impacts of technological implementation on the productivity and quality of seed potato tubers","authors":"Y. Setiyo, I. Gunam, B. A. Harsojuwono, K. A. Yuliadhi","doi":"10.37855/jah.2020.v22i03.49","DOIUrl":"https://doi.org/10.37855/jah.2020.v22i03.49","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47840411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2020.v22i03.48
L. Zurbano, A. Bellere, Gilda de Asis
{"title":"Growth, yield and quality of bitter gourd (Momordica charantia L.) under organic fertilization schemes","authors":"L. Zurbano, A. Bellere, Gilda de Asis","doi":"10.37855/jah.2020.v22i03.48","DOIUrl":"https://doi.org/10.37855/jah.2020.v22i03.48","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48641563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2020.v22i03.52
A. Ndereyimana, A. N. Niyokuri, B. Waweru, B. Kagiraneza, P. Rukundo, G. Hagenimana
{"title":"Yield response of watermelon (Citrullus lanatus Thunb.) cultivars to varied fertilizer rates in Rwanda","authors":"A. Ndereyimana, A. N. Niyokuri, B. Waweru, B. Kagiraneza, P. Rukundo, G. Hagenimana","doi":"10.37855/jah.2020.v22i03.52","DOIUrl":"https://doi.org/10.37855/jah.2020.v22i03.52","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46046037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2021.v23i02.29
Shekofeh Farahmand, Nematollah Ettemadi, B. Baninasab, Rahim Amirikhah
{"title":"Cold hardiness in bermudagrass cultivars as affected by the sequential trinexapac-ethyl application during growing season","authors":"Shekofeh Farahmand, Nematollah Ettemadi, B. Baninasab, Rahim Amirikhah","doi":"10.37855/jah.2021.v23i02.29","DOIUrl":"https://doi.org/10.37855/jah.2021.v23i02.29","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41785251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2021.v23i02.21
K. D. Ameta, R. Dubey, R. Kaushik, B. Chhipa, K. Rajawat
{"title":"Fertigation schedules and NPK doses influence growth and yield of tomato under polyhouse condition","authors":"K. D. Ameta, R. Dubey, R. Kaushik, B. Chhipa, K. Rajawat","doi":"10.37855/jah.2021.v23i02.21","DOIUrl":"https://doi.org/10.37855/jah.2021.v23i02.21","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41499391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.37855/jah.2021.v23i02.33
N. Islam, Sakif Zaman, Atiqur Rahman Shaon, S. Choudhury
{"title":"Effect of different growing environments on growth, yield and quality attributes of strawberry","authors":"N. Islam, Sakif Zaman, Atiqur Rahman Shaon, S. Choudhury","doi":"10.37855/jah.2021.v23i02.33","DOIUrl":"https://doi.org/10.37855/jah.2021.v23i02.33","url":null,"abstract":"","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41700581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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