New anti-inflammatory Triterpene Esters and glycosides from Alstonia scholaris.

Abstract

Phytochemical studies on the ethanolic extract of aerial parts of Alstoniascholaris lead to the isolation of two new triterpenoid of the lanostanetype,lanosta 5ene,24-ethyl-3-O-β-D-glucopyranoside(1), lanosta,5ene,24-ethyl-3-O-β-D-glucopyranosideester(2)and new ursane type triterpenoidmethylester, 12-ursene-2,3,18,19-tetrol,28 acetate (nighascholarene)(3),together with seven known triterpenes, betuline,triterpeneof the lupane type, alstoprenyol(4), 3β-hydroxy-28-β-acetoxy-5-olea triterpene (5),α-amyrin acetate (6), α-amyrin (7), lupeol acetate (8), 3β-hydroxy-24-nor-urs-4,12,28-triene triterpene (9) and ursolic acid (l0). The triterpenoid structures of these colorless compounds were deduced from the 1H and 13C-NMR data, and in particular from the application of two-dimensional 1H, 13C correlation experiments as well as by comparison with reported literature data.This study deals with isolation and structural elucidation ofnaturalnewtriterpenoidestersandglycosides with anti-inflammatory activity.