M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria
{"title":"Isolation and characterization of novel gene TaSSRP differentially expressed in wheat (Triticum aestivum L.) genotypes under heat stress","authors":"M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria","doi":"10.31742/ijgpb.82.2.12","DOIUrl":null,"url":null,"abstract":"Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Genetics and Plant Breeding","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31742/ijgpb.82.2.12","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.
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