Diagnosis of leaf bacterial diseases of coffee reveals the prevalence of halo blight

IF 1.2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Ciencia E Agrotecnologia Pub Date : 2021-06-25 DOI:10.1590/1413-7054202145000121
Melina Korres Raimundi, R. M. Souza, A. R. Figueira, Gustavo Matheus Silva, Ana Carolina de Paula Santos, Sarah S. C. Guimarães
{"title":"Diagnosis of leaf bacterial diseases of coffee reveals the prevalence of halo blight","authors":"Melina Korres Raimundi, R. M. Souza, A. R. Figueira, Gustavo Matheus Silva, Ana Carolina de Paula Santos, Sarah S. C. Guimarães","doi":"10.1590/1413-7054202145000121","DOIUrl":null,"url":null,"abstract":"ABSTRACT The diagnosis of foliar bacterial diseases in coffee (Coffea arabica), such as halo blight (Pseudomonas syringae pv. garcae), bacterial leaf spot (P. syringae pv. tabaci), bacterial blight (P. cichorii), and dark leaf spot (Robbsia andropogonis), is considered a challenge for plant pathologists. The misidentification has been occurring when the diagnosis is solely based on symptoms and biochemical properties. Thus, the objective of this study was to identify and differentiate species and pathovars of Pseudomonas pathogenic to coffee plants, enabling a survey of the occurrence of these bacteria in the main producing regions of Minas Gerais state, Brazil. Firstly, the pathogenicity of the isolates was confirmed by inoculation in C. arabica cv. Catuaí Vermelho IAC 99. Then, biochemical analyses, combined with, repetitive element-polymerase chain reaction (rep-PCR) and phylogeny based on rpoD gene sequences were used to characterize 84 Pseudomonas isolates from coffee crops and nurseries. Based on rpoD-phylogeny, 73 isolates were identified as P. syringae pv. garcae, five as P. syringae pv. tabaci and six as P. cichorii. The rep-PCR results suggest a high genetic variability in populations of Pseudomonas syringae pv. garcae and P. cichorii. This is the first report of the occurrence of bacterial leaf spot (P. syringae pv. tabaci) in the coffee-producing filed in Minas Gerais State. The findings confirmed the prevalence of P. syringae pv. garcae in coffee production fields in the State and the generated knowledge will contribute for the development of species-specific primers for the identification and detection of this pathogen.","PeriodicalId":10188,"journal":{"name":"Ciencia E Agrotecnologia","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ciencia E Agrotecnologia","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/1413-7054202145000121","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 1

Abstract

ABSTRACT The diagnosis of foliar bacterial diseases in coffee (Coffea arabica), such as halo blight (Pseudomonas syringae pv. garcae), bacterial leaf spot (P. syringae pv. tabaci), bacterial blight (P. cichorii), and dark leaf spot (Robbsia andropogonis), is considered a challenge for plant pathologists. The misidentification has been occurring when the diagnosis is solely based on symptoms and biochemical properties. Thus, the objective of this study was to identify and differentiate species and pathovars of Pseudomonas pathogenic to coffee plants, enabling a survey of the occurrence of these bacteria in the main producing regions of Minas Gerais state, Brazil. Firstly, the pathogenicity of the isolates was confirmed by inoculation in C. arabica cv. Catuaí Vermelho IAC 99. Then, biochemical analyses, combined with, repetitive element-polymerase chain reaction (rep-PCR) and phylogeny based on rpoD gene sequences were used to characterize 84 Pseudomonas isolates from coffee crops and nurseries. Based on rpoD-phylogeny, 73 isolates were identified as P. syringae pv. garcae, five as P. syringae pv. tabaci and six as P. cichorii. The rep-PCR results suggest a high genetic variability in populations of Pseudomonas syringae pv. garcae and P. cichorii. This is the first report of the occurrence of bacterial leaf spot (P. syringae pv. tabaci) in the coffee-producing filed in Minas Gerais State. The findings confirmed the prevalence of P. syringae pv. garcae in coffee production fields in the State and the generated knowledge will contribute for the development of species-specific primers for the identification and detection of this pathogen.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
咖啡叶细菌性疾病的诊断揭示了晕染病的流行
摘要咖啡(Coffea arabica)叶片细菌性疾病的诊断,如晕斑病(Pseudomonas syringae pv.gagae)、细菌性叶斑病(P.syringae.tabaci)、白叶枯病(P.cichoii)和暗叶斑病,被认为是植物病理学家面临的挑战。当诊断仅仅基于症状和生化特性时,就会出现错误识别。因此,本研究的目的是鉴定和区分对咖啡植物致病的假单胞菌的种类和致病性,从而能够调查这些细菌在巴西米纳斯吉拉斯州主要产区的发生情况。首先,通过接种阿拉伯C.CatuaíVermelho IAC 99,证实了分离株的致病性。然后,利用生物化学分析,结合重复元件聚合酶链式反应(rep-PCR)和基于rpoD基因序列的系统发育,对来自咖啡作物和苗圃的84株假单胞菌进行了鉴定。根据rpoD系统发育,鉴定出73个分离株为丁香P.syringae pv。garcae,5个为P.syringae pv。tabaci和6个为P.cichoii。rep-PCR结果表明丁香假单胞菌pv群体具有较高的遗传变异性。garcae和P.cichoii。这是米纳斯吉拉斯州咖啡生产区首次出现细菌性叶斑病(P.syringae pv.tabaci)的报告。研究结果证实了丁香假单胞菌pv。该州咖啡生产领域的garcae及其产生的知识将有助于开发用于鉴定和检测该病原体的物种特异性引物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Ciencia E Agrotecnologia
Ciencia E Agrotecnologia 农林科学-农业综合
CiteScore
2.30
自引率
9.10%
发文量
19
审稿时长
6-12 weeks
期刊介绍: A Ciência e Agrotecnologia, editada a cada 2 meses pela Editora da Universidade Federal de Lavras (UFLA), publica artigos científicos de interesse agropecuário elaborados por membros da comunidade científica nacional e internacional. A revista é distribuída em âmbito nacional e internacional para bibliotecas de Faculdades, Universidades e Instituições de Pesquisa.
期刊最新文献
Molecular characterization of common bean accessions using microsatellite markers Stocking density of red tilapia (Oreochromis sp.) reared in a commercial biofloc system in Colombia Edible coatings with avocado oil on the quality of ‘Tommy Atkins’ mangoes Identification of volatile compounds in salep (Serapias vomeracea) tubers and effects of harvest time and drying method on composition variation Adaptability and stability of mungbean genotypes in the Mid-North of Mato Grosso, Brazil
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1