Research progress in the detection of DNA and protein interaction

Abstract

From bacteria to mammalian cells, protein-DNA interactions are indispensable for all living organisms. In order to perform cell growth, differentiation, division, and regulation of cell function when stimulated by the external environment, DNA-encoded genetic information must be accurately transcribed, which is extremely important for the growth, development, and evolution of organisms. Manipulating the interaction of protein and DNA through biotechnology can modulate the expression of certain virulence genes, so as to contributing to the treatment of diseases. With the deepening of scientific research, more and more scientific technologies are applied in the field of protein and DNA interaction research. In this paper, the principles, advantages, disadvantages and applications of commonly used techniques in the study of protein-DNA interactions were reviewed, which will help researchers evaluate and select appropriate methods in their researches. Key words: Protein; DNA; Interaction; Detection technology