ASCERTAINING THE EFFECT OF BAP (6- BENZYL AMINO PURINE) ON ROSE (Rosa spp) THROUGH MICROPROPAGATION

Q4 Agricultural and Biological Sciences Plant Cell Biotechnology and Molecular Biology Pub Date : 2022-11-09 DOI:10.56557/pcbmb/2022/v23i37-387924

Shakira Afnan Mohammad Sadar, Sowmiya Madhaiyan, Sneaha Mahadevan, Shalini Govindaraj, Sindhuja Ravichandran, Sowmiya Jayakumar, Tharshenee Umapathy, Vidhya Lakshmanasamy, Sampath Sarangapani, Ashok Subiramaniyan, Arul Ganesh Thangaraj, V. Muthu, R. Moorthy, Sala Murugan, Shunmugavadivel Thangavel, Sarankumar Chandran

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Abstract

In this investigation, Rose nodal explants from healthy shoots were grown on a base MS medium supplemented with various BAP concentrations (6-Benzyl Amino Purine). It was determined from the various concentrations that the shoot initiation and development were best at the concentration of 3 mg/lit. From the third day to a one-month interval, the various stages of shoot development were examined. It was revealed that callus initiation begins on day 10, axillary shoot initiation on day 2, and that growth and development begin on day 3 and last for a month, respectively. Additionally, the established healthy shoot culture will be kept up and given root initiation treatment using rooting media. The ideal rooting media concentration will be fixed for better root initiation and development.

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6-苄基氨基嘌呤(BAP)对玫瑰(Rosa spp)微繁的影响

在本研究中，来自健康芽的玫瑰节外植体在补充有各种BAP浓度（6-苄基氨基嘌呤）的基础MS培养基上生长。从不同的浓度可以确定，芽的起始和发育在3mg/lit的浓度下最好。从第三天到间隔一个月，对芽发育的各个阶段进行了检查。结果表明，愈伤组织在第10天开始分化，腋芽在第2天分化，生长发育分别在第3天开始并持续一个月。此外，将保持已建立的健康芽培养基，并使用生根培养基进行生根处理。理想的生根培养基浓度将被固定，以更好地生根和发育。

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Plant Cell Biotechnology and Molecular Biology Agricultural and Biological Sciences-Horticulture

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