Hemotological Changes in Bombyx mori (Lepidoptera: Bombycidae) Infected by Nosema bombycis

Q2 Agricultural and Biological Sciences The Journal of Agricultural and Urban Entomology Pub Date : 2021-11-04 DOI:10.3954/JAUE21-10

S. Nematollahian, M. Bagheri, Z. Yousefi, A. Khezrian, A. Zahmatkesh

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引用次数: 1

Abstract

ABSTRACT Pébrine is the most important disease of silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), and is caused by Nosema bombycis (Nägeli) (Dissociodihaplophasida: Nosematidae). We investigated the effect of N. bombycis infection on the total hemocyte count (THC) and the differential hemocyte count (DHC) in hemolymph of a disease-resistant line (Line 104) and a susceptible line (Line 153) reared under optimum conditions. Fifth-instar silkworms were inoculated with a microsporidia spore suspension. Hemolymph was collected on the second, fourth and sixth days after inoculation, and THC and DHC of infected and uninfected silkworms were enumerated. Significantly higher THC was observed in the infected silkworms than the uninfected silkworms, but there was no significant difference in THC between lines. A significant difference in THC among days since inoculation in both lines was observed, with the average THC being higher on the second day since inoculation. DHC were significantly different among lines and days since inoculation. Granulocytes and plasmatocytes were the most numerous in Line 104, whereas granulocytes, plasmatocytes and prohemocytes were the most numerous in Line 153. Nosema infection decreased the percentage of all hemocytes, but increased the percentage of adipohemocytes and plasmatocytes. This study contributes to an improved understanding of the effects of microporidian infection that affects the sericulture industry.

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家蚕（鳞翅目：家蚕科）感染小夜蛾后的血液学变化

摘要/ ABSTRACT摘要:蚕蛹病是家蚕(鳞翅目:家蚕科)最重要的疾病，由家蚕微虫(Nägeli) (Dissociodihaplophasida: Nosematidae)引起。我们研究了在最佳条件下饲养的一个抗病品系(line 104)和一个敏感品系(line 153)，瓢虫感染对其血淋巴中总血细胞计数(THC)和差异血细胞计数(DHC)的影响。用微孢子虫孢子悬浮液接种5龄家蚕。接种后第2天、第4天和第6天采集血淋巴，分别对感染和未感染的家蚕进行THC和DHC计数。四氢大麻酚(THC)侵染家蚕显著高于未侵染家蚕，但品系间无显著差异。两系接种后各天THC差异显著，接种后第2天THC平均值较高。接种后不同品系、不同天数的DHC差异显著。粒细胞和浆细胞在Line 104中数量最多，而粒细胞、浆细胞和原血细胞在Line 153中数量最多。小虫感染降低了所有血细胞的百分比，但增加了脂肪血细胞和浆细胞的百分比。本研究有助于提高对影响蚕桑产业的微孢子虫感染的认识。

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来源期刊

The Journal of Agricultural and Urban Entomology Agricultural and Biological Sciences-Insect Science

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Journal of Agricultural and Urban Entomology (JAUE) (Journal of Agricultural Entomology, Jan 1984 - Oct 1998 volumes 1-15) is published under the auspices of the South Carolina Entomological Society (SCES). The Journal publishes contributions of original research concerning insects and other arthropods of agricultural and urban importance to include those affecting humans, livestock, poultry, and wildlife. JAUE is particularly dedicated to the publication of articles and notes pertaining to applied entomology, although it will accept suitable contributions of a fundamental nature related to agricultural and urban entomology.