Gene Expression of Monoterpene Synthases Is Affected Rhythmically during the Day in Lavandula angustifolia Flowers

Eleftheria Seira, Stefania Poulaki, C. Hassiotis, Stylianos Poulios, K. Vlachonasios
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引用次数: 1

Abstract

Lavender essential oil (EO) is widely used for medicinal purposes. The significant monoterpenes’ abundance of linalool and linalool acetate accounts for more than 50% of lavender EO compounds. Monoterpenes synthesis differs throughout plant development as a result of the differential gene expression patterns in distinct cell types. Previously, we have reported that the chemical composition of Lavandula angustifolia cv. etherio EO was affected by diurnal harvest time. The aim of this was to evaluate if the gene expression of lavender monoterpenes synthases is altered during the day length and correlated with the accumulation of the major components of lavender EO. The relative expression of linalool synthase (LaLINS), limonene synthase (LaLIMS) and terpene synthase-like (LaTPS-l) was recorded in flowers at the 3rd to 5th stage every 3 h during two consecutive days using quantitative real-time PCR. The composition of the lavender EO was also monitored during the day length using GC-MS analysis. Our results indicate that the expression of genes involved in the synthesis of lavender EO, including linalool and limonene synthases, is accompanied by oscillations, picking at mid-day and leading to linalool acetate accumulation in the afternoon. In conclusion, the monoterpenes synthase expression in lavender flowers is rhythmically affected during the day, leading to a higher accumulation of EO compounds in the afternoon. These results will be helpful to monitor the biosynthesis of lavender EO to ensure a high-quality product. Furthermore, the outcome of this study will be useful for breeding programs in the lavender field to modulate the biosynthesis of linalool and linalool acetate during the flowering harvest period.
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薰衣草花单萜合成酶基因表达受昼夜节律性影响
薰衣草精油(EO)被广泛用于医药用途。芳樟醇和乙酸芳樟醇的单萜含量占薰衣草EO化合物的50%以上。由于不同细胞类型中不同的基因表达模式,单萜的合成在整个植物发育过程中有所不同。以前,我们曾报道过狭叶薰衣草的化学成分受到昼夜收获时间的影响。其目的是评估薰衣草单萜合酶的基因表达是否在一天中发生改变,并与薰衣草EO主要成分的积累相关。在连续两天的第3至第5阶段,每3小时用实时定量PCR记录花中芳樟醇合成酶(LaLINS)、柠檬烯合成酶(LaLIMS)和萜类合成酶(LaTPS-l)的相对表达。薰衣草EO的组成也在一天中使用GC-MS分析进行监测。我们的研究结果表明,参与薰衣草EO合成的基因,包括芳樟醇和柠檬烯合成酶的表达,伴随着振荡,在中午采摘,并导致芳樟醇乙酸酯在下午积累。总之,薰衣草花中的单萜合酶表达在白天受到有节奏的影响,导致EO化合物在下午积累更高。这些结果将有助于监测薰衣草EO的生物合成,以确保高质量的产品。此外,这项研究的结果将有助于薰衣草领域的育种计划,以在开花收获期调节芳樟醇和乙酸芳樟醇的生物合成。
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