Evaluation of oxidative stress induction in rats following exposure to silver nanorods

IF 2.8 4区医学 Q2 TOXICOLOGY Toxicology Mechanisms and Methods Pub Date : 2017-01-25 DOI:10.1080/15376516.2016.1274351

Harikiran Lingabathula, N. Yellu

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引用次数: 5

Abstract

Abstract The study investigated the oxidative stress induction by the 10 and 25 nm silver nanorods (SNRs) following intra-tracheal instillation in rats after 1 day, 1 week, 1 month and 3 months post instillation periods at 1 and 5 mg/kg b.w. doses. The blood was withdrawn by retro orbital plexus method after exposure periods and different oxidative stress markers were estimated. The results showed that the both sizes of SNRs induced increased levels of malondialdehyde (MDA) and depleted glutathione (GSH) levels after 1 day and 1 week post exposure periods. The 10 and 25 nm SNRs at both doses displayed that significantly reduced levels of superoxide dismutase (SOD) and catalase following 1 day and 1 week post exposure periods. Also, the results have shown that decrease in total antioxidant capacity (TAC) of both sizes of SNRs significantly following 1 day and 1 week post exposure periods, indicating the oxidative stress induction by SNRs. In spite, there were no significant changes in oxidative stress markers following 1 month and 3 months post exposure periods may be due to recovery. The increased levels of MDA and decreased levels of GSH, SOD, catalase and TAC activity are strongly associated to ROS production and lipid peroxidation, suggesting the induction of oxidative stress in rats. The 10 nm SNRs at 5 mg/kg b.w. dose exposures in rats have shown greater changes in all oxidative stress parameters, indicating the greater induction of oxidative stress when compared with the 25 nm SNRs, representing the size–dose-dependent induction of oxidative stress of SNRs.

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银纳米棒暴露后大鼠氧化应激诱导的评价

摘要研究了10和25对氧化应激的诱导作用 nm银纳米棒（SNRs）在1 滴注后第1天、第1周、第1个月和第3个月 mg/kg体重剂量。暴露期后通过眶后丛法抽血，并评估不同的氧化应激标志物。结果表明，两种大小的SNRs在1 第天和暴露后1周。10和25 两种剂量的纳米SNRs显示，1 第天和暴露后1周。此外，研究结果表明，两种尺寸的SNRs的总抗氧化能力（TAC）的下降显著遵循1 第天和暴露后1周，表明SNRs诱导的氧化应激。尽管如此，暴露后1个月和3个月氧化应激标志物没有显著变化，这可能是由于恢复所致。MDA水平的升高和GSH、SOD、过氧化氢酶和TAC活性的降低与ROS的产生和脂质过氧化密切相关，表明大鼠氧化应激的诱导。10 5时的nm SNR 大鼠的mg/kg b.w.剂量暴露显示出所有氧化应激参数的更大变化，表明与25 nm SNRs，代表SNRs氧化应激的大小-剂量依赖性诱导。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.