Ethanolic Extract of Ocimum sanctum Linn. Inhibits Cell Migration of Human Lung Adenocarcinoma Cells (A549) by Downregulation of Integrin αvβ3, α5β1, and VEGF

IF 2.3 Q3 PHARMACOLOGY & PHARMACY Scientia Pharmaceutica Pub Date : 2022-10-31 DOI:10.3390/scipharm90040069
Ulayatul Kustiati, S. Ergün, S. Karnati, D. Nugrahaningsih, D. L. Kusindarta, H. Wihadmadyatami
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引用次数: 1

Abstract

Adenocarcinoma lung cancer is a type of non-small cell lung carcinoma (NSCLC), which accounts for 85% of lung cancer incidence globally. The therapies that are being applied, both conventional therapies and antibody-based treatments, are still found to have side effects. Several previous studies have demonstrated the ability of the ethanolic extract of Ocimum sanctum Linn. (EEOS) as an ethnomedicine with anti-tumor properties. The aim of this study was to determine the effect of Ocimum sanctum Linn. ethanolic extract in inhibiting the proliferation, angiogenesis, and migration of A549 cells (NSCLC). The adhesion as well as the migration assay was performed. Furthermore, enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of αvβ3 integrins, α5β1 integrins, and VEGF. The cells were divided into the following treatment groups: control (non-treated/NT), positive control (AP3/inhibitor β3 80 µg/mL), cisplatin (9 µg/mL), and EEOS at concentrations of 50, 70, 100, and 200 µg/mL. The results showed that EEOS inhibits the adhesion ability and migration of A549 cells, with an optimal concentration of 200 µg/mL. ELISA testing showed that the group of A549 cells given EEOS 200 µg/mL presented a decrease in the optimal expression of integrin α5β1, integrin αvβ3, and VEGF.
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香蒲乙醇提取物。整合素αvβ3、α5β1和VEGF下调对人肺腺癌细胞(A549)迁移的抑制作用
腺癌肺癌症是一种非小细胞肺癌(NSCLC),占全球癌症发病率的85%。正在应用的疗法,包括传统疗法和基于抗体的疗法,仍然被发现有副作用。先前的几项研究已经证明了香蒲乙醇提取物的能力。(EEOS)作为一种具有抗肿瘤特性的民族医学。本研究的目的是为了确定香蒲的作用。乙醇提取物抑制A549细胞(NSCLC)的增殖、血管生成和迁移。进行粘附和迁移测定。此外,酶联免疫吸附试验(ELISA)用于测量αvβ3整合素、α5β1整合素和VEGF的表达。将细胞分为以下治疗组:对照组(未治疗/NT)、阳性对照组(AP3/抑制剂β3 80µg/mL)、顺铂(9µg/mL)和浓度分别为50、70、100和200µg/mL的EEOS。结果表明,EEOS抑制A549细胞的粘附能力和迁移,最佳浓度为200µg/mL。ELISA测试显示,给予200µg/mL EEOS的A549细胞组整合素α5β1、整合素αvβ3和VEGF的最佳表达降低。
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来源期刊
Scientia Pharmaceutica
Scientia Pharmaceutica Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.60
自引率
4.00%
发文量
67
审稿时长
10 weeks
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