Chemical characterisation of sulfated polysaccharides from the red seaweed Centroceras clavulatum and their in vitro immunostimulatory and antioxidant properties

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2023-04-10 DOI:10.1016/j.fhfh.2023.100135

Uzeme P. Aluta , Ademola Z. Aderolu , Ismail O. Ishola , Mohammad Alyassin , Gordon A. Morris , Olumayokun A. Olajide

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Abstract

Phycocolloids have aroused research interest due to their remarkable functional properties, including immunostimulatory and antioxidant activities. In this study, the yield of Centroceras clavulatum sulfated polysaccharides (CCSP) was 4.36% and had a sulfate content of 9.42%. The main monosaccharide units of CCSP were galactose (25.23 ± 0.81 mg/l) and glucose (4.32 ± 0.31 mg/l). Specifically, FT-IR spectrum of CCSP revealed a peak at 843 cm⁻¹ which indicates the occurrence of sulfate groups on C-4 of galactose. CCSP stimulated Carp Leukocyte Culture (CLC) cell line to generate reactive oxygen species (ROS) and nitric oxide (NO) in a dose-dependent manner. Remarkably, exposure of CLC cells to CCSP at a dose of 12.5 µg/ml significantly increased superoxide dismutase activity and reduced malondialdehyde level. These results suggest that CCSP could stimulate CLC cells without compromising the intracellular antioxidant system, hence, may have potential applications as nutraceuticals.

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红海藻硫酸化多糖的化学性质及其体外免疫刺激和抗氧化性能

藻胶体因其具有免疫刺激和抗氧化活性等显著的功能特性而引起了人们的研究兴趣。本研究中，棒状心虫硫酸化多糖(CCSP)的产率为4.36%，硫酸盐含量为9.42%。CCSP的主要单糖单位为半乳糖(25.23±0.81 mg/l)和葡萄糖(4.32±0.31 mg/l)。具体来说，CCSP的FT-IR光谱显示在843 cm−1处有一个峰，表明半乳糖的C-4上存在硫酸盐基团。CCSP刺激鲤鱼白细胞培养(CLC)细胞系产生活性氧(ROS)和一氧化氮(NO)呈剂量依赖性。值得注意的是，12.5µg/ml剂量的CCSP显著增加了CLC细胞的超氧化物歧化酶活性，降低了丙二醛水平。这些结果表明，CCSP可以刺激CLC细胞而不损害细胞内抗氧化系统，因此可能具有潜在的营养保健应用。

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