Detection of Nigrospora sphaerica in the Philippines and the susceptibility of three Hylocereus species to reddish-brown spot disease

IF 0.4 4区农林科学 Q4 HORTICULTURE Journal of the Professional Association for Cactus Development Pub Date : 2020-09-30 DOI:10.56890/jpacd.v22i.321

J. D. Taguiam, E. Evallo, J. Bengoa, R. Maghirang, M. Balendres

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引用次数: 7

Abstract

Diseases are among the major problems that negatively affect dragon fruit profitability worldwide. Diseases of dragon fruit in the Philippines are yet to be identified and reported. This study elucidates the causal agent of a disease infecting stems of dragon fruit grown in Los Baños, Laguna, Philippines. The fungus was isolated and identified as Nigrospora sp. based on morphological and cultural characteristics in potato dextrose agar medium. Using the DNA sequence of the internal transcribed spacer (ITS) gene region, isolate MBDF0016b was identified as Nigrospora sphaerica. The Philippines strain was closely related to the Malaysian strain, which also causes reddish-brown spot in dragon fruit (H. polyrhizus), and to other N. sphaerica isolates from other host-plant species. Nigrospora sphaerica MBDF0016b was pathogenic to H. megalanthus, H. undatus, and H. polyrhizus in detached stem and glasshouse assays. The same fungus was re-isolated from the inoculated stems and thus, establishing Koch’s postulate. This paper is the first confirmed scientific record of a dragon fruit disease in the Philippines and the first report of N. sphaerica as a dragon fruit pathogen causing reddishbrown spot disease in H. megalanthus.

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菲律宾球形黑孢子虫的检测及三种黑孢子虫对红褐色斑疹病的易感性

病害是影响火龙果全球收益的主要问题之一。菲律宾火龙果的病害尚未查明和报告。本研究阐明了一种疾病感染的火龙果茎生长在Los Baños，拉古纳，菲律宾。根据马铃薯葡萄糖琼脂培养基的形态特征和培养特征分离鉴定为黑孢菌。利用ITS基因区DNA序列，鉴定分离物MBDF0016b为球形黑孢菌。菲律宾菌株与马来西亚菌株密切相关，马来西亚菌株也引起火龙果(H. polyrhizus)的红褐色斑点，并与其他寄主植物种的其他球形北丝孢菌分离株密切相关。球形黑孢菌MBDF0016b在离体茎和温室试验中对大花蓟马、黑孢蓟马和多根蓟马具有致病性。从接种的茎中重新分离出同样的真菌，从而建立了科赫的假设。本文是菲律宾首次证实的火龙果病的科学记录，也是首次报道了引起桃红褐斑病的火龙果病原菌N. sphaerica。

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来源期刊

Journal of the Professional Association for Cactus Development Agricultural and Biological Sciences-Plant Science

CiteScore

1.10

自引率

33.30%

发文量

期刊介绍： The editors of the Journal of the Professional Association for Cactus Development, are very excited to be a part of the excellent editorial committee and to work together to create the synergism between scientists, growers, legislators, and business people so vital to the development of this industry to serve the people of arid lands.