Effect of branched chain amino acid on apoptosis of neurons after focal cerebral ischemia in rats

Q4 Nursing 中华临床营养杂志 Pub Date : 2019-04-30 DOI:10.3760/CMA.J.ISSN.1674-635X.2019.02.010

F. Yan, Peng Sun, Huishou Zhao, Chong Huang, Xiong Guo, Xiaomeng Zhang

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Abstract

Objective To investigate the regulatory effect of branched chain amino acids(BCAA) on the expression of apoptosis related proteins after cerebral ischemia reperfusion injury and the protective effects of BCAA on ischemic brain injury in rats. Methods 40 male SD rats were randomly divided into normal diet group(n=20)and branched chain amino acid (BCAA) group(n=20)according to the random number table, and each group was randomly divided into control group (n=6), sham operation group (n=6) and model group (n=8) which used suture method to make ischemia reperfusion model. After modeling, modified Neurological Severity Scores (mNSS) was used to access the neurological impairment degree of 2, 6, 24, 48 and 72 h in each group. The expression of apoptosis related proteins (Cleaved, Bax/Bcl-2) after 72 h was detected by the method of immune protein imprinting (Caspase3) and compared between normal diet group and BCAA group. Results Compared with the normal diet rats, the mNSS of BCAA diet rats after modeling at 2, 6, 24, 48, 72 h decreased(11.35±2.78 vs. 7.15±2.41, P=0.019; 9.35±1.75 vs. 5.82±1.17, P=0.002; 6.11±1.16 vs. 4.39±1.46, P=0.048; 5.87±1.32 vs. 3.55±1.94, P=0.036; 4.98±2.24 vs. 2.09±1.33, P=0.022). The expression of cleaved caspase3 protein and the ratio of Bax/Bcl-2 decreased in BCAA group. Conclusion BCAA can alleviate the apoptosis of rats after ischemia and reperfusion, reduce the damage of nerve function, and has a positive protective effect on ischemic brain injury. Key words: Branched chain amino acid; Cerebral ischemia reperfusion; Apoptosis

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支链氨基酸对大鼠局灶性脑缺血后神经元凋亡的影响

目的探讨支链氨基酸(BCAA)对大鼠脑缺血再灌注损伤后凋亡相关蛋白表达的调控作用及对缺血性脑损伤的保护作用。方法将40只雄性SD大鼠按随机数字表法随机分为正常饮食组(n=20)和支链氨基酸(BCAA)组(n=20)，每组随机分为对照组(n=6)、假手术组(n=6)和模型组(n=8)，采用缝合法制作缺血再灌注模型。建模完成后，采用改良神经功能严重程度评分法(mNSS)计算各组在2、6、24、48、72 h时的神经功能损害程度。采用免疫蛋白印迹法(Caspase3)检测72 h后细胞凋亡相关蛋白(Cleaved、Bax/Bcl-2)的表达，并比较正常饮食组和BCAA组的差异。结果与正常饮食大鼠比较，造模后2、6、24、48、72 h BCAA饮食大鼠的mNSS降低(11.35±2.78∶7.15±2.41,P=0.019;9.35±1.75 vs. 5.82±1.17,P=0.002;6.11±1.16 vs. 4.39±1.46,P=0.048;5.87±1.32 vs. 3.55±1.94,P=0.036;(4.98±2.24∶2.09±1.33,P=0.022)。BCAA组caspase3裂解蛋白表达降低，Bax/Bcl-2比值降低。结论BCAA能减轻大鼠缺血再灌注后的细胞凋亡，减轻神经功能损伤，对缺血性脑损伤具有积极的保护作用。关键词:支链氨基酸;脑缺血再灌注;细胞凋亡

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来源期刊

中华临床营养杂志 Nursing-Nutrition and Dietetics

CiteScore

0.20

自引率

0.00%

发文量

2282

期刊介绍： The Chinese Journal of Clinical Nutrition was founded in 1993. It is the first professional academic journal (bimonthly) in my country co-sponsored by the Chinese Medical Association and the Chinese Academy of Medical Sciences to disseminate information on clinical nutrition support, nutrient metabolism, the impact of nutrition support on outcomes and "cost-effectiveness", as well as translational medicine and nutrition research. It is also a professional journal of the Chinese Medical Association's Parenteral and Enteral Nutrition Branch. The purpose of the Chinese Journal of Clinical Nutrition is to promote the rapid dissemination of knowledge on nutrient metabolism and the rational application of parenteral and enteral nutrition, focusing on the combination of multidisciplinary and multi-regional field investigations and clinical research. It mainly reports on nutritional risk screening related to the indications of parenteral and enteral nutrition support, "cost-effectiveness" research on nutritional drugs, consensus on clinical nutrition, guidelines, expert reviews, randomized controlled studies, cohort studies, glycoprotein and other nutrient metabolism research, systematic evaluation of clinical research, evidence-based case reports, special reviews, case reports and clinical experience exchanges, etc., and has a special column on new technologies related to the field of clinical nutrition and their clinical applications.