The Decrease in Spectrum Intensity of ESBL Spectra after Exposure to Clavulanic Acid in Nosocomial Urinary Tract Infected Escherichia coli Analysed by VITEK® MS

Q3 Health Professions Chiang Mai University journal of natural sciences Pub Date : 2022-10-18 DOI:10.12982/cmujns.2022.061

Pratchaya Wisutthithada, Thanathida Sirilueangtrakul, Ponkit Suwannapong, Rutjapong Nongmak, P. Khamnoi, T. Sastraruji, S. Sookkhee

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Abstract

Abstract The present study aimed to investigate the influence of clavulanic acid on the spectrum intensity of Extended Spectrum β- Lactamase (ESBL) indicative spectra in the nosocomial urinary tract infected Escherichia coli. Two hundred nosocomial urinary tract infected E. coli isolates collected between 2017-2019 were recruited. Their antibiotic susceptibilities and ESBL productions were then determined. The effect of clavulanic acid contained in amoxicillin-clavulanic acid towards the change of spectra intensity after being exposed was also determined to identify their ESBL indicative spectra. Results revealed that these nosocomial isolates exhibited a low percent resistance after being tested to piperacillin-tazobactam, meropenem, doripenem, and gentamicin. VITEK® MS analysis demonstrated five E. coli species-specific spectra including 4363, 5097, 5381, 6255, and 9065 Dalton. After exposure to ceftazidime alone, and co-exposure to ceftazidime and amoxicillin-clavulanic acid, six ceftazidime-susceptible, and five ceftazidime-stress spectra, 5381, 6412, 7870, 8876,10139 and 10301 Dalton and 3578, 6226, 6316, 7274, and 8370 Dalton were significantly detected, respectively. Whereas two ceftazidime-resistant spectra, 4613 and 9715 Dalton, exhibited a significantly decreased intensity after determined in the high ESBL producing group after co-exposure to ceftazidime and clavulanic acid, respectively. In conclusion, an ESBL inhibitor or clavulanic acid could significantly decrease the spectrum intensity of two spectra 4613 and 9715 Dalton after being co-exposed to ceftazidime and clavulanic acid and these spectra were suspected as ESBL indicative spectra in the high ESBL producing nosocomial E. coli isolates. Keywords: Extended spectrum β- Lactamase indicative spectra, clavulanic acid exposure, nosocomial urinary tract infection, Escherichia coli, VITEK® MS

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VITEK®质谱法分析医院尿路感染大肠埃希菌暴露于克拉维酸后ESBL光谱强度的降低

摘要本研究旨在探讨克拉维酸对医院感染大肠埃希菌尿路扩展谱β-内酰胺酶(ESBL)指示谱谱强度的影响。招募了2017-2019年间收集的200株医院尿路感染大肠杆菌。然后测定它们的抗生素敏感性和ESBL产量。测定阿莫西林-克拉维酸中所含克拉维酸对暴露后光谱强度变化的影响，鉴定其ESBL指示光谱。结果显示，这些医院分离株对哌拉西林-他唑巴坦、美罗培南、多利培南和庆大霉素的耐药率较低。VITEK®质谱分析显示了5个大肠杆菌物种特异性光谱，包括4363、5097、5381、6255和9065道尔顿。头孢他啶单独暴露、头孢他啶与阿莫西林-氯维酸共暴露后，分别检测到5381、6412、7870、8876、10139、10301道尔顿和3578、6226、6316、7274、8370道尔顿的6个头孢他啶敏感谱和5个头孢他啶应激谱。而两个抗头孢他啶的光谱，4613和9715道尔顿，在分别暴露于头孢他啶和克拉维酸后的高ESBL产生组中显示出显著降低的强度。综上所示，一种ESBL抑制剂或克拉维酸可显著降低ceftazidime和clavulanic acid共暴露后的4613和9715 Dalton两个光谱的光谱强度，这些光谱可能是ESBL高产源医院性大肠杆菌分离株的ESBL指示光谱。关键词:扩展谱β-内酰胺酶指示谱，克拉维酸暴露，院内尿路感染，大肠杆菌，VITEK®质谱

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Chiang Mai University journal of natural sciences Health Professions-Health Professions (miscellaneous)

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1.70

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0.00%

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