In vitro CYTOTOXICITY AND APOPTOTIC INDUCING EFFECTS OF Dimera acutipus ON MCF-7 BREAST CANCER CELLS

Abstract

Cancer is a complex multistage diseases process. Surgery and radio therapy are failing to cure certain kind of malignancies chiefly because of the tumour is already disseminated for this reason the chemotherapeutic agents are sought. Therefore, there is need to find out a new class of anti-cancer agents with no or less toxic from medicinal herbal plant sources. Hence it is of interest to investigate the in-vitro analysis cytotoxicity and apoptotic inducing efficacy of the plant extract studies were undertaken. The in-vitro analysis of cytotoxicity of the chloroform extract of Dimera acutipus were analysed by using the MTT assay and the apoptotic analysis was performed by the flow cytometric analysis. The present study is to explore the cytotoxicity as well as the apoptotic effects of the chloroform extract of aerial parts of Dimera acutipus in normal Vero cell lines and human breast cancer cell lines. The main aim of this study is to evaluate the cytotoxicity of the plant extract in Vero and MCF-7 cell lines was undertaken. Our results indicate that non cytotoxic nature of the chloroform extract of Dimera acutipus in appropriate cell lines. In conclusion the anti-apoptotic analysis clearly indicates the chloroform extract of Dimera acutipus has the ability of cell cycle arresting potential in conclusion the present investigation clearly envisages a plant extract could be a strong anticancer agent.