Wound healing synergy in Wistar albino rats via green synthesized nanoparticles and topical antibiotic neomycin

Abstract

Raise of antimicrobial resistance and lack of development in novel antibiotics leads to complications in infection control for wound healing. In perspective to search for best alternatives, antibacterial activity of nanomaterials has shown promising strategy, however concentration dependent toxicity became challenge thereof. In this context, green synthesis protocols of nano materials provide benefits of biocompatibility due to presence of bioactive compounds and also economical with proven efficiency. Further nano-antibiotic combinations may enhance antibacterial efficacy by synergetic action and allows to reduce the dosage of both agents. In the current work, nano-antibiotic gels are prepared using green synthesized nanoparticles (Ag & ZnO NPs) with the combination of antibiotic neomycin and also assessed in-vivo wound healing activity on Wistar albino rats. From the results of the tested formulations, combinational formulations exhibited enhanced and speedier wound contraction (92–96%) with prominent synergetic action when compared with neomycin alone (84%) or nanoparticles alone (82–86%) in a 14-day study. These results demonstrated that green-nano-antibiotic combinational formulations provides prominent avenue to combat the multi drug resistant bacteria without toxicity issue.