Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-02-09 DOI: 10.1016/j.onano.2025.100237

Thanida Chuacharoen , Carlos E. Astete , Cristina M. Sabliov

Resveratrol loaded zein nanoparticles (Res ZNPs) were synthesized to deliver resveratrol to bone cells for inhibiting the proliferation of osteosarcoma cells. Zein chemically cross-linked with alendronate as a bone-targeting agent using EDC and NHS chemistry was also synthesized and the cross-linking was confirmed using Fourier-Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Subsequently, Res ZNPs with and without alendronate were synthesized and characterized. The particles measured 273 to 294 nm with a narrow polydispersity index, and a zeta potential of -29 to -33 mV, respectively as evaluated by dynamic light scattering. The particles showed spherical morphology imaged by transmission electron microscopy and the entrapment efficiency and loading capacity were 63.0 % and 13.6 % for Res ZNPs and 69.1 % and 21.0 % for Res ZNPs with alendronate, respectively. Furthermore, the entrapped resveratrol of both systems was released in a three-phase manner under physiological condition (phosphate-buffered saline, PBS) at 37 °C over 24 h. Both systems exhibited suppression of osteosarcoma MG-63 cell proliferation and the inhibition rate was found slightly higher for targeted, Res ZNPs with alendronate. This research suggested that Res ZNPs conjugated with alendronate could be a candidate for effective bone-targeting delivery systems to inhibit the proliferation of osteosarcoma cells.

{"title":"Resveratrol loaded zein nanoparticles for inhibiting proliferation of osteosarcoma cells: Synthesis, characterization, release profile, and cytotoxicity","authors":"Thanida Chuacharoen , Carlos E. Astete , Cristina M. Sabliov","doi":"10.1016/j.onano.2025.100237","DOIUrl":"10.1016/j.onano.2025.100237","url":null,"abstract":"<div><div>Resveratrol loaded zein nanoparticles (Res ZNPs) were synthesized to deliver resveratrol to bone cells for inhibiting the proliferation of osteosarcoma cells. Zein chemically cross-linked with alendronate as a bone-targeting agent using EDC and NHS chemistry was also synthesized and the cross-linking was confirmed using Fourier-Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Subsequently, Res ZNPs with and without alendronate were synthesized and characterized. The particles measured 273 to 294 nm with a narrow polydispersity index, and a zeta potential of -29 to -33 mV, respectively as evaluated by dynamic light scattering. The particles showed spherical morphology imaged by transmission electron microscopy and the entrapment efficiency and loading capacity were 63.0 % and 13.6 % for Res ZNPs and 69.1 % and 21.0 % for Res ZNPs with alendronate, respectively. Furthermore, the entrapped resveratrol of both systems was released in a three-phase manner under physiological condition (phosphate-buffered saline, PBS) at 37 °C over 24 h. Both systems exhibited suppression of osteosarcoma MG-63 cell proliferation and the inhibition rate was found slightly higher for targeted, Res ZNPs with alendronate. This research suggested that Res ZNPs conjugated with alendronate could be a candidate for effective bone-targeting delivery systems to inhibit the proliferation of osteosarcoma cells.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100237"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osteogenic differentiation of mesenchymal stem cells in cell-laden culture of self-assembling peptide hydrogels

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-28 DOI: 10.1016/j.onano.2025.100235

Faye Fouladgar , Robert Powell , Vishalakshi Irukuvarjula , Akhila Joy , Xiao Li , Neda Habibi

Mesenchymal stem cell (MSC) osteogenic differentiation requires scaffolds to support multiple stages of growth and differentiation signals. Fluorenyl-9-methoxycarbonyl diphenylalanine (Fmoc-FF) peptides self-assemble to create 3D nanofibers. Here, we cultured MSC in 2D and 3D Fmoc-FF layers to support their osteogenic differentiation. The stiffness of the hydrogels was tunable between 100 and 10,000 Pa which allows precise modulation of the cellular microenvironment. Scaffold stiffness impacted cell viability which softer scaffolds (100 Pa) favored higher viability. MSC formed spheroids in 3D hydrogel and showed spread morphology in 2D overlayers. Our results demonstrate that the Fmoc-FF 3D cultures significantly enhanced osteogenic differentiation, as evidenced by increased calcium deposition, elevated phosphatase activity, and the secretion of osteocalcin. We propose that the peptides provide integrin-binding sites that activate a cytoplasmic feedback loop essential for differentiation. These findings suggest that self-assembled Fmoc-FF peptide hydrogels, is a promising platform for bone tissue engineering applications.

{"title":"Osteogenic differentiation of mesenchymal stem cells in cell-laden culture of self-assembling peptide hydrogels","authors":"Faye Fouladgar , Robert Powell , Vishalakshi Irukuvarjula , Akhila Joy , Xiao Li , Neda Habibi","doi":"10.1016/j.onano.2025.100235","DOIUrl":"10.1016/j.onano.2025.100235","url":null,"abstract":"<div><div>Mesenchymal stem cell (MSC) osteogenic differentiation requires scaffolds to support multiple stages of growth and differentiation signals. Fluorenyl-9-methoxycarbonyl diphenylalanine (Fmoc-FF) peptides self-assemble to create 3D nanofibers. Here, we cultured MSC in 2D and 3D Fmoc-FF layers to support their osteogenic differentiation. The stiffness of the hydrogels was tunable between 100 and 10,000 Pa which allows precise modulation of the cellular microenvironment. Scaffold stiffness impacted cell viability which softer scaffolds (100 Pa) favored higher viability. MSC formed spheroids in 3D hydrogel and showed spread morphology in 2D overlayers. Our results demonstrate that the Fmoc-FF 3D cultures significantly enhanced osteogenic differentiation, as evidenced by increased calcium deposition, elevated phosphatase activity, and the secretion of osteocalcin. We propose that the peptides provide integrin-binding sites that activate a cytoplasmic feedback loop essential for differentiation. These findings suggest that self-assembled Fmoc-FF peptide hydrogels, is a promising platform for bone tissue engineering applications.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100235"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143222762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carrageenan bionanocomposite films incorporating Ag and Zn-Doped CeO₂ nanoparticles for active food packaging applications

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-18 DOI: 10.1016/j.onano.2025.100234

Damar Rastri Adhika , Gita Genecya , Alvin Annayya Habibah , An Naas Amalia Rahardja Putri , Ubed Sonai Fahruddin Arrozi

The increasing demand for sustainable and safe food packaging has led to the exploration of bio-based materials and advanced packaging technologies. This study investigates the incorporation of silver (Ag) and zinc (Zn) doped cerium oxide nanoparticles (CeO₂ NPs) into carrageenan-based bionanocomposite films to enhance their antimicrobial properties, mechanical strength, and hydrophobicity. The synthesis of CeO₂ NPs, doped with varying concentrations of Ag and Zn, was achieved using the green synthesis method with green tea extract as a reducing agent. Characterization techniques such as X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta potential analysis confirmed the successful doping and stability of the nanoparticles. The bionanocomposites were evaluated for their mechanical properties, water contact angle, and antibacterial activity against Escherichia coli and Bacillus cereus. Mechanical testing revealed that the addition of CeO₂ NPs, particularly Ag-doped CeO₂ NPs, significantly improved the tensile strength and Young's modulus of the bionanocomposites. Hydrophobicity assessments showed that Zn-doped CeO₂ NPs enhanced water resistance compared to Ag-doped CeO₂ NPs, making them more suitable for food packaging applications. Zn and Ag-doped CeO₂ NPs exhibited superior antibacterial activity compared to undoped CeO₂ NPs, with 20 wt% Ag-doped NPs showing the highest antibacterial activity compared to Amoxicillin as positive control and other variations. The study concludes that Zn and Ag-doped CeO₂ NPs are promising additives for developing effective and sustainable active food packaging materials.

{"title":"Carrageenan bionanocomposite films incorporating Ag and Zn-Doped CeO₂ nanoparticles for active food packaging applications","authors":"Damar Rastri Adhika , Gita Genecya , Alvin Annayya Habibah , An Naas Amalia Rahardja Putri , Ubed Sonai Fahruddin Arrozi","doi":"10.1016/j.onano.2025.100234","DOIUrl":"10.1016/j.onano.2025.100234","url":null,"abstract":"<div><div>The increasing demand for sustainable and safe food packaging has led to the exploration of bio-based materials and advanced packaging technologies. This study investigates the incorporation of silver (Ag) and zinc (Zn) doped cerium oxide nanoparticles (CeO₂ NPs) into carrageenan-based bionanocomposite films to enhance their antimicrobial properties, mechanical strength, and hydrophobicity. The synthesis of CeO₂ NPs, doped with varying concentrations of Ag and Zn, was achieved using the green synthesis method with green tea extract as a reducing agent. Characterization techniques such as X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta potential analysis confirmed the successful doping and stability of the nanoparticles. The bionanocomposites were evaluated for their mechanical properties, water contact angle, and antibacterial activity against <em>Escherichia coli</em> and <em>Bacillus cereus</em>. Mechanical testing revealed that the addition of CeO₂ NPs, particularly Ag-doped CeO₂ NPs, significantly improved the tensile strength and Young's modulus of the bionanocomposites. Hydrophobicity assessments showed that Zn-doped CeO₂ NPs enhanced water resistance compared to Ag-doped CeO₂ NPs, making them more suitable for food packaging applications. Zn and Ag-doped CeO₂ NPs exhibited superior antibacterial activity compared to undoped CeO₂ NPs, with 20 wt% Ag-doped NPs showing the highest antibacterial activity compared to Amoxicillin as positive control and other variations. The study concludes that Zn and Ag-doped CeO₂ NPs are promising additives for developing effective and sustainable active food packaging materials.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100234"},"PeriodicalIF":0.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic dissemination

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-15 DOI: 10.1016/j.onano.2025.100233

Sara Micheli , Caterina Piunti , Elisa Varaschin , Marianna Peditto , Maria Luz Suarez , Marco Sorgato , Elisa Cimetta

Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.

{"title":"Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic dissemination","authors":"Sara Micheli , Caterina Piunti , Elisa Varaschin , Marianna Peditto , Maria Luz Suarez , Marco Sorgato , Elisa Cimetta","doi":"10.1016/j.onano.2025.100233","DOIUrl":"10.1016/j.onano.2025.100233","url":null,"abstract":"<div><div>Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100233"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing in Vitro anti-metastatic efficacy and deep penetration into tumor spheroid of docetaxel-loaded liposomes via size optimization for prostate cancer treatment

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-04 DOI: 10.1016/j.onano.2024.100231

Saksorn Klibaim, Nutthanit Thumrongsiri, Natsorn Watcharadulyarat, Walailuk Chonniyom, Prattana Tanyapanyachon, Paweena Dana, Nattika Saengkrit

Castration-resistant prostate cancer (CRPC) presents a formidable challenge due to its aggressiveness and limited treatment options. Loading the drug docetaxel (DTX) into liposomes is a potential alternative approach to improve its efficacy. Several studies have reported that size optimization can improve drug efficacy in other cancer models. Therefore, this study explored the potential of size-optimization of docetaxel-loaded liposomes (LDTX) to improve in vitro efficacy against CRPC. The impacts of LDTX size (<100 nm and 100–200 nm) on cellular uptake, cytotoxicity in both monolayer and three-dimensional (3D) tumor spheroid models, and anti-metastatic effects were investigated. The results showed significant cellular internalization improvement with smaller LDTX, leading to better cytotoxicity in a monolayer cell culture than with larger LDTX. Moreover, smaller liposomes enabled deep penetration into the tumor spheroid, mimicking the tumor microenvironment and effectively eradicating cancer cells inside the spheroid. Interestingly, smaller liposomes also enhanced the anti-metastatic phenotype by inhibiting cancer cell invasion. The findings demonstrate that liposomes size is crucial in enhancing the efficacy of anti-cancer drugs. Therefore, size optimization is essential for developing highly effective formulations, requiring thorough investigation to identify the optimal liposomes size for specific applications.

{"title":"Enhancing in Vitro anti-metastatic efficacy and deep penetration into tumor spheroid of docetaxel-loaded liposomes via size optimization for prostate cancer treatment","authors":"Saksorn Klibaim, Nutthanit Thumrongsiri, Natsorn Watcharadulyarat, Walailuk Chonniyom, Prattana Tanyapanyachon, Paweena Dana, Nattika Saengkrit","doi":"10.1016/j.onano.2024.100231","DOIUrl":"10.1016/j.onano.2024.100231","url":null,"abstract":"<div><div>Castration-resistant prostate cancer (CRPC) presents a formidable challenge due to its aggressiveness and limited treatment options. Loading the drug docetaxel (DTX) into liposomes is a potential alternative approach to improve its efficacy. Several studies have reported that size optimization can improve drug efficacy in other cancer models. Therefore, this study explored the potential of size-optimization of docetaxel-loaded liposomes (LDTX) to improve <em>in vitro</em> efficacy against CRPC. The impacts of LDTX size (<100 nm and 100–200 nm) on cellular uptake, cytotoxicity in both monolayer and three-dimensional (3D) tumor spheroid models, and anti-metastatic effects were investigated. The results showed significant cellular internalization improvement with smaller LDTX, leading to better cytotoxicity in a monolayer cell culture than with larger LDTX. Moreover, smaller liposomes enabled deep penetration into the tumor spheroid, mimicking the tumor microenvironment and effectively eradicating cancer cells inside the spheroid. Interestingly, smaller liposomes also enhanced the anti-metastatic phenotype by inhibiting cancer cell invasion. The findings demonstrate that liposomes size is crucial in enhancing the efficacy of anti-cancer drugs. Therefore, size optimization is essential for developing highly effective formulations, requiring thorough investigation to identify the optimal liposomes size for specific applications.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100231"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-04 DOI: 10.1016/j.onano.2025.100232

Suwipa Ungphaiboon , Sutasinee Ardhanwanich , Duangkhae Maneenuan , Sirirat Pinsuwan , Pawika Mahasawat

This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2.78 mV. Encapsulation of VCO enhanced mushroom tyrosinase activity by 3-fold and exhibited lower cytotoxicity to B16-F10 cells compared to VCO alone. Moreover, a positive correlation was observed between the increase in intracellular tyrosinase activity and the concentrations of VCO (r = 0.8366) and VCO-loaded liposomes (r = 0.4794) in B16-F10 cells, while a negative correlation (r = -0.0545) was found for liposomes without VCO. A hair and eyebrow-darkening gel containing both VCO and VCO-loaded liposomes further enhanced mushroom tyrosinase activity by 283.33 ± 26.58 %. These findings suggest that VCO-loaded liposomes may serve as novel and effective nano-scale carriers for VCO in cosmetic applications.

{"title":"Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity","authors":"Suwipa Ungphaiboon , Sutasinee Ardhanwanich , Duangkhae Maneenuan , Sirirat Pinsuwan , Pawika Mahasawat","doi":"10.1016/j.onano.2025.100232","DOIUrl":"10.1016/j.onano.2025.100232","url":null,"abstract":"<div><div>This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2.78 mV. Encapsulation of VCO enhanced mushroom tyrosinase activity by 3-fold and exhibited lower cytotoxicity to B16-F10 cells compared to VCO alone. Moreover, a positive correlation was observed between the increase in intracellular tyrosinase activity and the concentrations of VCO (<em>r</em> = 0.8366) and VCO-loaded liposomes (<em>r</em> = 0.4794) in B16-F10 cells, while a negative correlation (<em>r</em> = -0.0545) was found for liposomes without VCO. A hair and eyebrow-darkening gel containing both VCO and VCO-loaded liposomes further enhanced mushroom tyrosinase activity by 283.33 ± 26.58 %. These findings suggest that VCO-loaded liposomes may serve as novel and effective nano-scale carriers for VCO in cosmetic applications.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100232"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan-Coated silver nanoparticles with various floral honey bioreductors: A promising nonalcoholic hand gel sanitizer formulation

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-01 DOI: 10.1016/j.onano.2024.100228

Saidun Fiddaroini , Kurnia Indu , Luailik Madaniyah , Suci Amalia , Aulanni'am , Moh. Farid Rahman , Akhmad Sabarudin

Antimicrobial resistance represents a critical global health challenge, necessitating innovative strategies to combat resistant pathogens. In this study, silver nanoparticles (AgNPs) were synthesized using honey as a bioreductant and coated with oligochitosan derived from the depolymerization of low-molecular-weight chitosan. The synthesis employed eco-friendly methods, with characterization performed via UV–Vis spectroscopy, FTIR, TEM, EDX, XRD, and LCHRMS. AgNPs synthesized with Ceiba pentandra honey exhibited an average particle size of 11.71 nm, demonstrating high antibacterial activity when coated with oligochitosan. The 10 % AgNPs-Chitosan-based hand gel sanitizer formulation achieved inhibition zones of 14.84 ± 0.40 mm against Staphylococcus aureus and 11.16 ± 0.73 mm against Pseudomonas aeruginosa. The hand gel sanitizer formulation exhibited stable pH (4.0–4.3), high resistance to syneresis at 5 °C and 40 °C, and superior antibacterial efficacy compared to alcohol-based hand gel sanitizers. Dermatological assessments confirmed the formulation's safety, and Artemia salina toxicity tests revealed the highest LC₅₀ value (2,648.97 ppm) for AgNPs derived from C. pentandra honey. This work provides an eco-friendly, efficient method for AgNP synthesis with strong potential for biomedical and environmental applications, including their use in hand gel sanitizers to reduce pathogen transmission in various settings, contributing to the advancement of green nanotechnology.

{"title":"Chitosan-Coated silver nanoparticles with various floral honey bioreductors: A promising nonalcoholic hand gel sanitizer formulation","authors":"Saidun Fiddaroini , Kurnia Indu , Luailik Madaniyah , Suci Amalia , Aulanni'am , Moh. Farid Rahman , Akhmad Sabarudin","doi":"10.1016/j.onano.2024.100228","DOIUrl":"10.1016/j.onano.2024.100228","url":null,"abstract":"<div><div>Antimicrobial resistance represents a critical global health challenge, necessitating innovative strategies to combat resistant pathogens. In this study, silver nanoparticles (AgNPs) were synthesized using honey as a bioreductant and coated with oligochitosan derived from the depolymerization of low-molecular-weight chitosan. The synthesis employed eco-friendly methods, with characterization performed via UV–Vis spectroscopy, FTIR, TEM, EDX, XRD, and LC<img>HRMS. AgNPs synthesized with <em>Ceiba pentandra</em> honey exhibited an average particle size of 11.71 nm, demonstrating high antibacterial activity when coated with oligochitosan. The 10 % AgNPs-Chitosan-based hand gel sanitizer formulation achieved inhibition zones of 14.84 ± 0.40 mm against <em>Staphylococcus aureus</em> and 11.16 ± 0.73 mm against <em>Pseudomonas aeruginosa.</em> The hand gel sanitizer formulation exhibited stable pH (4.0–4.3), high resistance to syneresis at 5 °C and 40 °C, and superior antibacterial efficacy compared to alcohol-based hand gel sanitizers. Dermatological assessments confirmed the formulation's safety, and <em>Artemia salina</em> toxicity tests revealed the highest LC<sub>50</sub> value (2,648.97 ppm) for AgNPs derived from <em>C. pentandra</em> honey. This work provides an eco-friendly, efficient method for AgNP synthesis with strong potential for biomedical and environmental applications, including their use in hand gel sanitizers to reduce pathogen transmission in various settings, contributing to the advancement of green nanotechnology.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100228"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of bilastine nanosuspension for enhanced dissolution in orodispersible films

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-01 DOI: 10.1016/j.onano.2024.100230

Sarah Adnan Oudah , Eman B.H. Al-Khedairy

Bilastine, a second-generation antihistamine, is commonly prescribed for managing allergic rhinoconjunctivitis and urticaria due to its prolonged action. However, its therapeutic potential is constrained by poor water solubility and low oral bioavailability. This study aimed to enhance bilastine dissolution and patient compliance by formulating a nanosuspension-based orodispersible film (ODF). An anti-solvent precipitation method was employed to produce nanosuspension using different hydrophilic stabilizers (Soluplus®, Poloxamer 188, and PEG 6000). The influence of formulation parameters, such as the stabilizer ratio, the anti-solvent ratio, stirring speed, and the stabilizer type, on particle size and polydispersity index (PDI) was optimized using an experimental design approach. The optimal formulation, with a 1:1 stabilizer-to-drug ratio using Soluplus®, a 6:1 anti-solvent to solvent ratio, and a stirring rate of 820 rpm, yielded nanoparticles with a mean particle size of 83.8 nm and a narrow PDI of 0.019. This formulation also significantly enhanced the drug's dissolution rate in phosphate buffer pH 6.8, releasing 92.02% of bilastine within 90 minutes. Further characterization of the lyophilized nanoparticles using FESEM, FTIR, and XRD, confirmed their amorphous nature and drug compatibility. The optimized nanosuspension was subsequently incorporated into ODFs via the solvent-casting technique, with the optimal film formulated with a 1:1 ratio of PVA and HPMC E5 as the film-forming polymers, demonstrating a rapid disintegration time of 18 seconds and releasing 93.16% of bilastine within 6 minutes. These results confirm the successful formulation of bilastine into ODFs, significantly improving its dissolution compared to the pure drug.

{"title":"Development and characterization of bilastine nanosuspension for enhanced dissolution in orodispersible films","authors":"Sarah Adnan Oudah , Eman B.H. Al-Khedairy","doi":"10.1016/j.onano.2024.100230","DOIUrl":"10.1016/j.onano.2024.100230","url":null,"abstract":"<div><div>Bilastine, a second-generation antihistamine, is commonly prescribed for managing allergic rhinoconjunctivitis and urticaria due to its prolonged action. However, its therapeutic potential is constrained by poor water solubility and low oral bioavailability. This study aimed to enhance bilastine dissolution and patient compliance by formulating a nanosuspension-based orodispersible film (ODF). An anti-solvent precipitation method was employed to produce nanosuspension using different hydrophilic stabilizers (Soluplus®, Poloxamer 188, and PEG 6000). The influence of formulation parameters, such as the stabilizer ratio, the anti-solvent ratio, stirring speed, and the stabilizer type, on particle size and polydispersity index (PDI) was optimized using an experimental design approach. The optimal formulation, with a 1:1 stabilizer-to-drug ratio using Soluplus®, a 6:1 anti-solvent to solvent ratio, and a stirring rate of 820 rpm, yielded nanoparticles with a mean particle size of 83.8 nm and a narrow PDI of 0.019. This formulation also significantly enhanced the drug's dissolution rate in phosphate buffer pH 6.8, releasing 92.02% of bilastine within 90 minutes. Further characterization of the lyophilized nanoparticles using FESEM, FTIR, and XRD, confirmed their amorphous nature and drug compatibility. The optimized nanosuspension was subsequently incorporated into ODFs via the solvent-casting technique, with the optimal film formulated with a 1:1 ratio of PVA and HPMC E5 as the film-forming polymers, demonstrating a rapid disintegration time of 18 seconds and releasing 93.16% of bilastine within 6 minutes. These results confirm the successful formulation of bilastine into ODFs, significantly improving its dissolution compared to the pure drug.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100230"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Material informatics-driven insights into brain cancer nanocarriers: A bibliometric comparison of PLGA vs. liposomes

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-01 DOI: 10.1016/j.onano.2024.100225

Brilly Andro Makalew , Syauqi Abdurrahman Abrori

This study explores a comparative analysis of PLGA nanoparticles and liposomes as potential carriers for brain cancer drug delivery, with a special focus on how material informatics enhances their design, biocompatibility, and drug release profiles to improve treatment efficacy and contribute to sustainable health outcomes.

The investigation employed a bibliometric analysis using Scopus and VOSviewer to uncover the role of material informatics in optimizing these nanocarriers. The analysis revealed that material informatics, particularly through the application of machine learning and molecular dynamics simulations, significantly optimizes the performance of both PLGA nanoparticles and liposomes.

The results highlighted distinct strengths of each nanocarrier: PLGA nanoparticles excel in biodegradability, while liposomes offer superior drug encapsulation capabilities. However, material informatics techniques bridged these enhancing drug release kinetics, stability, and biocompatibility. These improvements are crucial for effective delivery across the blood-brain barrier, a major challenge in brain cancer treatment.

The integration of computational modelling, machine learning, and high-throughput screening enabled by material informatics is shown to be a key factor in advancing the design and optimization of these nanocarriers. By leveraging these tools, researchers can develop more personalized and efficient drug delivery systems tailored to address the specific challenges of glioblastoma therapy, ultimately contributing to sustainable health outcomes

{"title":"Material informatics-driven insights into brain cancer nanocarriers: A bibliometric comparison of PLGA vs. liposomes","authors":"Brilly Andro Makalew , Syauqi Abdurrahman Abrori","doi":"10.1016/j.onano.2024.100225","DOIUrl":"10.1016/j.onano.2024.100225","url":null,"abstract":"<div><div>This study explores a comparative analysis of PLGA nanoparticles and liposomes as potential carriers for brain cancer drug delivery, with a special focus on how material informatics enhances their design, biocompatibility, and drug release profiles to improve treatment efficacy and contribute to sustainable health outcomes.</div><div>The investigation employed a bibliometric analysis using Scopus and VOSviewer to uncover the role of material informatics in optimizing these nanocarriers. The analysis revealed that material informatics, particularly through the application of machine learning and molecular dynamics simulations, significantly optimizes the performance of both PLGA nanoparticles and liposomes.</div><div>The results highlighted distinct strengths of each nanocarrier: PLGA nanoparticles excel in biodegradability, while liposomes offer superior drug encapsulation capabilities. However, material informatics techniques bridged these enhancing drug release kinetics, stability, and biocompatibility. These improvements are crucial for effective delivery across the blood-brain barrier, a major challenge in brain cancer treatment.</div><div>The integration of computational modelling, machine learning, and high-throughput screening enabled by material informatics is shown to be a key factor in advancing the design and optimization of these nanocarriers. By leveraging these tools, researchers can develop more personalized and efficient drug delivery systems tailored to address the specific challenges of glioblastoma therapy, ultimately contributing to sustainable health outcomes</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100225"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in lanthanide-based nanoparticle contrast agents for magnetic resonance imaging: Synthesis, characterization, and applications

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-01 DOI: 10.1016/j.onano.2024.100226

Azmi Aulia Rahmani , Qi Jia , Husein H. Bahti , Retna Putri Fauzia , Santhy Wyantuti

MRI is a powerful, non-invasive imaging technique with exceptional soft tissue contrast, requiring contrast agents to enhance sensitivity by shortening longitudinal (T₁) and transverse (T₂) relaxation times. While most clinical agents are chelate-based, their potential toxicity has driven the development of nanoparticle-based alternatives. Nanoparticles offer reduced toxicity, improved stability, prolonged circulation time, and better control over surface properties. Lanthanide-based nanoparticles, in particular, are promising due to their paramagnetic properties enhancing MRI contrast. The design of these nanoparticles focuses on optimizing size, shape, and colloidal stability with advances in synthesis techniques allowing for precise control over particle size, morphology, and stability to significantly influence relaxivity. Larger sizes increase r₂ values but may reduce stability, while anisotropic shapes enhance relaxivity compared to the more stable spheres. Surface modifications with functional polymers improve stability and prevent aggregation, optimizing imaging performance. As research progresses, lanthanide-based nanoparticles are poised to become crucial tools in radiology-driven cancer diagnosis and therapy, offering dual functionality for early detection, targeted treatment, and minimized off-target effects. However, these nanoparticles must be refined for tumour-specific diagnostic and therapeutic applications and undergo comprehensive safety evaluations before clinical trials.

{"title":"Recent advances in lanthanide-based nanoparticle contrast agents for magnetic resonance imaging: Synthesis, characterization, and applications","authors":"Azmi Aulia Rahmani , Qi Jia , Husein H. Bahti , Retna Putri Fauzia , Santhy Wyantuti","doi":"10.1016/j.onano.2024.100226","DOIUrl":"10.1016/j.onano.2024.100226","url":null,"abstract":"<div><div>MRI is a powerful, non-invasive imaging technique with exceptional soft tissue contrast, requiring contrast agents to enhance sensitivity by shortening longitudinal (T<sub>1</sub>) and transverse (T<sub>2</sub>) relaxation times. While most clinical agents are chelate-based, their potential toxicity has driven the development of nanoparticle-based alternatives. Nanoparticles offer reduced toxicity, improved stability, prolonged circulation time, and better control over surface properties. Lanthanide-based nanoparticles, in particular, are promising due to their paramagnetic properties enhancing MRI contrast. The design of these nanoparticles focuses on optimizing size, shape, and colloidal stability with advances in synthesis techniques allowing for precise control over particle size, morphology, and stability to significantly influence relaxivity. Larger sizes increase r₂ values but may reduce stability, while anisotropic shapes enhance relaxivity compared to the more stable spheres. Surface modifications with functional polymers improve stability and prevent aggregation, optimizing imaging performance. As research progresses, lanthanide-based nanoparticles are poised to become crucial tools in radiology-driven cancer diagnosis and therapy, offering dual functionality for early detection, targeted treatment, and minimized off-target effects. However, these nanoparticles must be refined for tumour-specific diagnostic and therapeutic applications and undergo comprehensive safety evaluations before clinical trials.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100226"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OpenNano最新文献