Acetoacetate Production from CO2 and Acetone with Acetone Carboxylase from Photosynthetic Bacteria Rhodobacter Capsulatus

Abstract

Carbon capture and utilization (CCU) technology is the process of capturing and converting CO₂ into useful materials for further usage. The CCU aims to convert the captured CO₂ into more valuable materials while maintaining the carbon neutrality of the production process. In this work, we have demonstrated the acetoacetate production from acetone and NaHCO₃ using acetone carboxylase (AC) in cell extract from photosynthetic bacteria Rhodobacter capsulatus strain SB1003 (Rb. capsulatus SB1003) cultured in a medium containing acetone and bicarbonate under anaerobic photoheterotrophic conditions as a catalyst in the presence of ATP. Steady acetoacetate production due to the carboxylation of acetone with NaHCO₃ was accomplished in the presence of ATP by using cell extract including AC from Rb. capsulatus SB1003. Moreover, steady acetoacetate production from acetone and gaseous CO₂ also was attained by using cell extract.