CHEMICAL COMPOSITION ANALYSIS AND EVALUATION OF ANTIOXIDANT, ANTIACETYLCHOLINESTERASE AND CYTOTOXICITY OF MURRAYA KOENIGII (L.) SPRENG FRUIT OIL

IF 0.6 Q4 FOOD SCIENCE & TECHNOLOGY Journal of microbiology, biotechnology and food sciences Pub Date : 2023-08-02 DOI:10.55251/jmbfs.6135

G. Ramnath, Shathya Jayaraman, R. Thirugnanasampandan, Bhuvaneswari Gunasekaran, Akila Gopalakrishnan

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Abstract

Plant derived essential oils are easily available source for healthcare purpose and it creates increased attention due to undesirable effects of chemically synthesized drugs. Murraya koenigii (L.) Spreng. raw fruit oil was extracted, and its chemical constituents was analyzed. Antioxidant and acetylcholinesterase inhibitory effect was tested under in vitro. MTT assay for evaluating cytotoxic effect on HeLa cells was done. β-phellandrene was identified as major compound in the extracted fruit oil. Scavenging of DPPH radical, inhibition of lipid peroxidation and protection of deoxyribose were found to be excellent. A dose dependent antiacetylcholinesterase activity of oil was observed with IC50 value as 448.12±0.02µg/mL. A significant growth inhibitory effect of oil on cervical cancer cells (HeLa) was measured with an IC50 value of 194.1±0.03µg/mL. Overall, the essential oil extracted from raw fruit of Murraya koenigii (L.) Spreng exhibited several biological properties, and it could be considered for plant-based medicine preparations.

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锦鲤化学成分分析、抗氧化、抗乙酰胆碱酯酶及细胞毒性评价春天的水果油

植物衍生精油很容易用于医疗保健，由于化学合成药物的不良影响，它引起了越来越多的关注。Murraya koenigii（L.）Spreng。对生果油进行了提取，并对其化学成分进行了分析。在体外条件下测试了抗氧化剂和乙酰胆碱酯酶的抑制作用。MTT法检测对HeLa细胞的细胞毒作用。β-水芹烯是提取的果油中的主要化合物。对DPPH自由基的清除、对脂质过氧化的抑制和对脱氧核糖的保护作用被发现是极好的。观察到油的剂量依赖性抗乙酰胆碱酯酶活性，IC50值为448.12±0.02µg/mL。测量到油对宫颈癌症细胞（HeLa）的显著生长抑制作用，IC50为194.1±0.03μg/mL。总体而言，从Murraya koenigii（L.）Spreng生果中提取的精油具有多种生物学特性，可考虑用于植物性药物制剂。

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来源期刊

Journal of microbiology, biotechnology and food sciences FOOD SCIENCE & TECHNOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

156

审稿时长

8 weeks

期刊介绍： The Journal of Microbiology, Biotechnology and Food Sciences is an Open Access, peer-reviewed online scientific journal published by the Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra). The major focus of the journal is regular publishing of original scientific articles, short communications and reviews about animal, plant and environmental microbiology (including bacteria, fungi, yeasts, algae, protozoa and viruses), microbial, animal and plant biotechnology and physiology, microbial, plant and animal genetics, molecular biology, agriculture and food chemistry and biochemistry, food control, evaluation and processing in food science and environmental sciences.