The adulteration and mislabeling of food ingredients, particularly meat products, pose a significant concern for consumers. In order to address this issue, it is crucial to use precise and sensitive methods for species identification, mainly when dealing with samples that contain a limited number of copies of DNA. The primary objective of this study is to design a digital nanoplate polymerase chain reaction (PCR) technique for the purpose of quantifying species identification. The primer used in this study was cytochrome b gene. The results showed that target DNA can be amplified using the digital PCR nanoplate until the lowest sample concentration of 0.0013 ng/µL. This study can be applied to enhance species identification processes in food samples by utilizing digital PCR nanoplates.
{"title":"PRECISION AND RELIABILITY OF NANOPLATE DIGITAL PCR SYSTEM FOR PORK DNA IDENTIFICATION AND QUANTIFICATION","authors":"Rini Hafzari, Annisa, Anita Kairani, Muchamad Nur Cholis, Listya Puspa Kirana, Nurul Huda Pangabean, Nurbaity Situmorang, Dwi Ratna Anjaning Kusuma Marpaung","doi":"10.55251/jmbfs.10691","DOIUrl":"https://doi.org/10.55251/jmbfs.10691","url":null,"abstract":"The adulteration and mislabeling of food ingredients, particularly meat products, pose a significant concern for consumers. In order to address this issue, it is crucial to use precise and sensitive methods for species identification, mainly when dealing with samples that contain a limited number of copies of DNA. The primary objective of this study is to design a digital nanoplate polymerase chain reaction (PCR) technique for the purpose of quantifying species identification. The primer used in this study was cytochrome b gene. The results showed that target DNA can be amplified using the digital PCR nanoplate until the lowest sample concentration of 0.0013 ng/µL. This study can be applied to enhance species identification processes in food samples by utilizing digital PCR nanoplates.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141107793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current investigation explores the antibacterial properties of Quercetin, a flavonoid found in plants, and lemon peel extract, renowned for its rich bioactive content, against multiple drug resistant bacteria. The study method involved the identification of multidrug-resistant bacteria through antimicrobial susceptibility testing (AST). Nine standard bacterial strains exhibited resistance to different antibiotics, with a MAR index exceeding 0.10, including Ampicillin, Vancomycin, Polymyxin B, Nitrofurantoin, Trimethoprim, and penicillins.In the present study bioactive compounds from lemon peel were extracted using two solvents, methanol and water, and their efficacy was assessed against drug-resistant bacteria. Minimum Inhibitory Concentration (MIC) values, determined by using the well plate method, revealed lower MIC values for the water extract (LPWE) compared to the methanol extract (LPME) and quercetin. For instance, the MIC for LPWE against E. hermanniensis ATCC 700323 was 140 mg/ml, whereas it was 50 mg/ml for quercetin & 1 mg/ml for LPME. From the results, it can be interpreted that Methanol extract was most effective against drug-resistant bacteria when compared to water extract and quercetin.Furthermore, GC-MS analysis was performed to analyze the chemical entities present in each extract. Given the activity of LPME, LPWE, and Quercetin against both Gram-negative bacteria and Gram-positive bacteria, these extracts hold promise as broad-spectrum antibacterial agents applicable in various fields such as medicine, food, and cosmetics.
目前的研究探讨了植物中的黄酮类化合物槲皮素和以富含生物活性而闻名的柠檬皮提取物对多种耐药细菌的抗菌特性。研究方法包括通过抗菌药物敏感性测试（AST）鉴定多重耐药细菌。九种标准细菌菌株表现出对不同抗生素的耐药性，MAR 指数超过 0.10，包括氨苄西林、万古霉素、多粘菌素 B、硝基呋喃妥因、甲氧苄啶和青霉素类。采用孔板法测定的最低抑菌浓度（MIC）值显示，与甲醇提取物（LPME）和槲皮素相比，水提取物（LPWE）的MIC值较低。例如，LPWE 对 E. hermanniensis ATCC 700323 的 MIC 值为 140 毫克/毫升，而槲皮素和 LPME 的 MIC 值分别为 50 毫克/毫升和 1 毫克/毫升。此外，还进行了气相色谱-质谱（GC-MS）分析，以分析每种提取物中存在的化学实体。鉴于 LPME、LPWE 和槲皮素对革兰氏阴性菌和革兰氏阳性菌的活性，这些提取物有望成为适用于医药、食品和化妆品等多个领域的广谱抗菌剂。
{"title":"COMPARATIVE STUDY OF QUERCETIN & LEMON PEEL EXTRACT ON MULTIPLE DRUG RESISTANT BACTERIA","authors":"Madhuri Patil, Renu Jaisinghani, Ravindra Sharma, Rohini Patil","doi":"10.55251/jmbfs.10099","DOIUrl":"https://doi.org/10.55251/jmbfs.10099","url":null,"abstract":"The current investigation explores the antibacterial properties of Quercetin, a flavonoid found in plants, and lemon peel extract, renowned for its rich bioactive content, against multiple drug resistant bacteria. The study method involved the identification of multidrug-resistant bacteria through antimicrobial susceptibility testing (AST). Nine standard bacterial strains exhibited resistance to different antibiotics, with a MAR index exceeding 0.10, including Ampicillin, Vancomycin, Polymyxin B, Nitrofurantoin, Trimethoprim, and penicillins.In the present study bioactive compounds from lemon peel were extracted using two solvents, methanol and water, and their efficacy was assessed against drug-resistant bacteria. Minimum Inhibitory Concentration (MIC) values, determined by using the well plate method, revealed lower MIC values for the water extract (LPWE) compared to the methanol extract (LPME) and quercetin. For instance, the MIC for LPWE against E. hermanniensis ATCC 700323 was 140 mg/ml, whereas it was 50 mg/ml for quercetin & 1 mg/ml for LPME. From the results, it can be interpreted that Methanol extract was most effective against drug-resistant bacteria when compared to water extract and quercetin.Furthermore, GC-MS analysis was performed to analyze the chemical entities present in each extract. Given the activity of LPME, LPWE, and Quercetin against both Gram-negative bacteria and Gram-positive bacteria, these extracts hold promise as broad-spectrum antibacterial agents applicable in various fields such as medicine, food, and cosmetics.\u0000 ","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141107496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kristina Trenzová, Michal Gross, Eva Vítová, Jromír Pořízka, P. Diviš
In this study, the dynamics of volatile compound alterations during the storage of ground roasted coffee in various packaging were investigated using solid-phase microextraction-gas chromatography-mass spectrometry. Each type of packaging underwent repeated opening and closing, simulating the gradual consumption behavior of the consumer who consumes ground coffee in smaller doses over time. The experimental results revealed that after 30 days of storage, 35 to 45% of the volatile substances originally present in the coffee were lost regardless of the type of packaging used. Notably, storing coffee in a paper sachet proved to be the least effective method, resulting in a loss of over 80% of all volatile coffee substances during this storage period.
{"title":"EXPLORING THE IMPACT OF DIFFERENT PACKAGING TYPES AND REPEATED PACKAGE OPENING ON VOLATILE COMPOUND CHANGES IN GROUND ROASTED COFFEE","authors":"Kristina Trenzová, Michal Gross, Eva Vítová, Jromír Pořízka, P. Diviš","doi":"10.55251/jmbfs.11022","DOIUrl":"https://doi.org/10.55251/jmbfs.11022","url":null,"abstract":"In this study, the dynamics of volatile compound alterations during the storage of ground roasted coffee in various packaging were investigated using solid-phase microextraction-gas chromatography-mass spectrometry. Each type of packaging underwent repeated opening and closing, simulating the gradual consumption behavior of the consumer who consumes ground coffee in smaller doses over time. The experimental results revealed that after 30 days of storage, 35 to 45% of the volatile substances originally present in the coffee were lost regardless of the type of packaging used. Notably, storing coffee in a paper sachet proved to be the least effective method, resulting in a loss of over 80% of all volatile coffee substances during this storage period.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141108743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Š. Ailer, L. Benešová, Mojmír Baroň, Lucia Galovičová
Food contamination is the result of over-reliance on fungicides in crop production. The presence of pesticide residues in agricultural commodities needs to be strategically solved by applying gentle agrotechnical procedures. The research aimed to evaluate the effect of the treatment of grapevine with fungicides on the content and mobility of 4 active substances in the leaves of the variety Hibernal. The experiment included a control variant treated with fungicides and an untreated variant. We focused on monitoring the active substances of fungicides used in the first half of the vegetation: azoxystrobin, cyflufenamid, difenoconazole, myclobutanil, and another 25 active substances, in terms of multi-residue analysis methodologies. Grape leaves for analysis were collected on two dates: in the phenological phase of berry development BBCH 73 and 14 days after grape harvest – BBCH 91. Samples were prepared according to STN EN 15662. For qualitative analyzes of residues, we used the QuEChERS method in combination with liquid (LC-MS/MS) and gas (GC-MS/MS) chromatography with tandem mass spectrometry. In the untreated variant, the contents of residues from both leaves collection were in undetectable values. In the leaf samples of the treated variant collected in BBCH 73, we established higher values of residues than the MRL determines, namely for azoxystrobin 0.083 mg/kg (MRL – 0.01 mg/kg). Azoxystrobin is the active substance of the Quadris max fungicide, and we applied this preparation 41 days before the first collection of leaves. In the analyzes of leaf sampling from the treated variant after grape harvest in BBCH 91, we found a decrease in the content of all monitored residues below detectable values. We consider the gradual degradation of residues in biological materials to be a very favorable result from the point of view of their impact on consumer health and the environment. Through visual observations of the vegetation, we found out that even the untreated variant had a perfect state of health. Hibernal is a variety suitable for biological production systems, and we assume that even in vintages with high pressure of fungal diseases, it is possible to maintain the health of the grapevine with ecological products. Using the appropriate grape variety combined with the right agrotechnical interventions makes it possible to use safer ecological practices to produce healthy food, maintaining sustainability and a healthy natural environment. The research will continue in the early Table grape varieties, where the vegetation period is shorter, and space for breaking down the residues is more difficult.
{"title":"MONITORING OF PESTICIDE RESIDUES IN GRAPEVINE LEAVES UNDER VARIANT FUNGAL DISEASE MANAGEMENT","authors":"Š. Ailer, L. Benešová, Mojmír Baroň, Lucia Galovičová","doi":"10.55251/jmbfs.11107","DOIUrl":"https://doi.org/10.55251/jmbfs.11107","url":null,"abstract":"Food contamination is the result of over-reliance on fungicides in crop production. The presence of pesticide residues in agricultural commodities needs to be strategically solved by applying gentle agrotechnical procedures. The research aimed to evaluate the effect of the treatment of grapevine with fungicides on the content and mobility of 4 active substances in the leaves of the variety Hibernal. The experiment included a control variant treated with fungicides and an untreated variant. We focused on monitoring the active substances of fungicides used in the first half of the vegetation: azoxystrobin, cyflufenamid, difenoconazole, myclobutanil, and another 25 active substances, in terms of multi-residue analysis methodologies. Grape leaves for analysis were collected on two dates: in the phenological phase of berry development BBCH 73 and 14 days after grape harvest – BBCH 91. Samples were prepared according to STN EN 15662. For qualitative analyzes of residues, we used the QuEChERS method in combination with liquid (LC-MS/MS) and gas (GC-MS/MS) chromatography with tandem mass spectrometry. In the untreated variant, the contents of residues from both leaves collection were in undetectable values. In the leaf samples of the treated variant collected in BBCH 73, we established higher values of residues than the MRL determines, namely for azoxystrobin 0.083 mg/kg (MRL – 0.01 mg/kg). Azoxystrobin is the active substance of the Quadris max fungicide, and we applied this preparation 41 days before the first collection of leaves. In the analyzes of leaf sampling from the treated variant after grape harvest in BBCH 91, we found a decrease in the content of all monitored residues below detectable values. We consider the gradual degradation of residues in biological materials to be a very favorable result from the point of view of their impact on consumer health and the environment. Through visual observations of the vegetation, we found out that even the untreated variant had a perfect state of health. Hibernal is a variety suitable for biological production systems, and we assume that even in vintages with high pressure of fungal diseases, it is possible to maintain the health of the grapevine with ecological products. Using the appropriate grape variety combined with the right agrotechnical interventions makes it possible to use safer ecological practices to produce healthy food, maintaining sustainability and a healthy natural environment. The research will continue in the early Table grape varieties, where the vegetation period is shorter, and space for breaking down the residues is more difficult.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141110469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study was to investigate the shell thickness of table eggs using a destructive method with a focus on the technique of sampling from three locations in the equatorial plane, the sharp and the blunt end of the egg. The subject of the study was the eggshell thickness with membranes of the influence of the sample taken on the egg in equatorial plane 1, equatorial plane 2, equatorial plane 3, at the sharp end and the blunt end of the egg, and a laying hen of breed Dominant aged 61 weeks (3 small-breedings) and 104 weeks (1 small-breeding). The measurement of eggshell thickness was carried out using a DIAL INDICATOR deviation meter, with an accuracy of 0.01 mm and a maximum thickness of 30 mm. The SAS statistical package, version 8.2, was used for statistical evaluation of the results. The influence of the location of the eggshell sample taken in 3 locations of the equatorial plane, at the sharp and blunt end of the egg on its thickness in the range of average values of 0.32 to 0.40 mm was not statistically significant (P>0.05). The influence of laying hen age was statistically significant (P≤0.05) by a higher eggshell thickness in the range of average values of 0.38 to 0.40 mm at 104 weeks compared with eggshell thickness of average values of 0.32 to 0.35 mm at 61 weeks. A strong linear positive correlation in eggshell thickness was found between two variables at all investigated sample locations on eggs laid by laying hens Dominant regardless of age. The overall results of eggshell thickness indicate that its evaluation is a complex process as an indicator of eggshell quality due to multifactorial external and internal influence. Considering the trend of lengthening the laying cycle in laying hens for environmental reasons, it is important to address the influence of age, housing system and breed suitability in relation to optimizing the uniformity of the thickness as part of the shell quality of the eggs.
{"title":"SAMPLING TECHNIQUE THE EGGSHELL THICKNESS","authors":"Michal Angelovič, L. Zeleňáková","doi":"10.55251/jmbfs.11056","DOIUrl":"https://doi.org/10.55251/jmbfs.11056","url":null,"abstract":"The aim of the study was to investigate the shell thickness of table eggs using a destructive method with a focus on the technique of sampling from three locations in the equatorial plane, the sharp and the blunt end of the egg. The subject of the study was the eggshell thickness with membranes of the influence of the sample taken on the egg in equatorial plane 1, equatorial plane 2, equatorial plane 3, at the sharp end and the blunt end of the egg, and a laying hen of breed Dominant aged 61 weeks (3 small-breedings) and 104 weeks (1 small-breeding). The measurement of eggshell thickness was carried out using a DIAL INDICATOR deviation meter, with an accuracy of 0.01 mm and a maximum thickness of 30 mm. The SAS statistical package, version 8.2, was used for statistical evaluation of the results. The influence of the location of the eggshell sample taken in 3 locations of the equatorial plane, at the sharp and blunt end of the egg on its thickness in the range of average values of 0.32 to 0.40 mm was not statistically significant (P>0.05). The influence of laying hen age was statistically significant (P≤0.05) by a higher eggshell thickness in the range of average values of 0.38 to 0.40 mm at 104 weeks compared with eggshell thickness of average values of 0.32 to 0.35 mm at 61 weeks. A strong linear positive correlation in eggshell thickness was found between two variables at all investigated sample locations on eggs laid by laying hens Dominant regardless of age. The overall results of eggshell thickness indicate that its evaluation is a complex process as an indicator of eggshell quality due to multifactorial external and internal influence. Considering the trend of lengthening the laying cycle in laying hens for environmental reasons, it is important to address the influence of age, housing system and breed suitability in relation to optimizing the uniformity of the thickness as part of the shell quality of the eggs.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141112068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Judita Lidiková, Natália Čeryová, J. Musilová, M. Bobko, A. Bobková, Alžbeta Demianová, Katarína Poláková, O. Grygorieva
The contents of biogenic and risk elements in the pomace of 6 grape cultivars (Blaufränkisch, Cabernet Franc, Cabernet Sauvignon, Devín, Dunaj, and Merlot) were analyzed in this study. Content of K, Ca, P, Mg, and Na was 11 750 – 19 070, 2 400 – 3 908, 1 678 – 2 684, 336 – 760, and 1.43 - 59.3 mg.kg-1 DM respectively. Content of Fe, Cu, Mn, Zn, Ni, Cr, and Co was 79.5 – 245, 6.18 – 48.4, 10.3 – 22.6, 4.19 – 17.0, 0.66 – 1.36, 0.058 – 2.33, and 0.42 – 0.63 mg.kg-1 DM respectively. Pb and Cd were detected in the grape pomace of Merlot (0.94 and 0.03 mg.kg-1 DM, respectively) and Cabernet Sauvignon (0.36 and 0.08 mg.kg-1 DM, respectively). The total content of Hg ranged from 0.0055 to 0.0080 mg.kg-1 DM. Analysis of variance showed that there are differences in the content of elements among cultivars. Grape pomace of cultivars Blaufränkisch and Cabernet Sauvignon could be characterized by the higher Fe, Mn, Zn, Ni, and Cr content, while grape pomace of cultivar Merlot by higher Mg content. With relatively low content of risk elements, grape pomace can serve as a good source of essential minerals in the human diet.
{"title":"BIOGENIC AND RISK ELEMENTS IN GRAPE POMACE OF DIFFERENT CULTIVARS","authors":"Judita Lidiková, Natália Čeryová, J. Musilová, M. Bobko, A. Bobková, Alžbeta Demianová, Katarína Poláková, O. Grygorieva","doi":"10.55251/jmbfs.11096","DOIUrl":"https://doi.org/10.55251/jmbfs.11096","url":null,"abstract":"The contents of biogenic and risk elements in the pomace of 6 grape cultivars (Blaufränkisch, Cabernet Franc, Cabernet Sauvignon, Devín, Dunaj, and Merlot) were analyzed in this study. Content of K, Ca, P, Mg, and Na was 11 750 – 19 070, 2 400 – 3 908, 1 678 – 2 684, 336 – 760, and 1.43 - 59.3 mg.kg-1 DM respectively. Content of Fe, Cu, Mn, Zn, Ni, Cr, and Co was 79.5 – 245, 6.18 – 48.4, 10.3 – 22.6, 4.19 – 17.0, 0.66 – 1.36, 0.058 – 2.33, and 0.42 – 0.63 mg.kg-1 DM respectively. Pb and Cd were detected in the grape pomace of Merlot (0.94 and 0.03 mg.kg-1 DM, respectively) and Cabernet Sauvignon (0.36 and 0.08 mg.kg-1 DM, respectively). The total content of Hg ranged from 0.0055 to 0.0080 mg.kg-1 DM. Analysis of variance showed that there are differences in the content of elements among cultivars. Grape pomace of cultivars Blaufränkisch and Cabernet Sauvignon could be characterized by the higher Fe, Mn, Zn, Ni, and Cr content, while grape pomace of cultivar Merlot by higher Mg content. With relatively low content of risk elements, grape pomace can serve as a good source of essential minerals in the human diet.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141108570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martin Tomáška, Maroš Drončovský, Miroslav Kološta
The popularity of goat's milk and its products is growing in Slovakia. Among the mandatory health safety features of raw goat's milk is the total bacterial count. It can be determined by the anchor plate-cultivation method, which is, however, tedious, and laborious. Laser flow cytometry is a modern alternative method for measuring the microbiological quality of raw milk. To use the results from laser flow cytometry method for legislative purposes, it is necessary to create a representative conversion of the primarily measured results obtained by this method into the CFU/mL scale. This work describes the creation of such a conversion for raw goat's milk, which will be valid for the Slovak region. After measuring several hundred individual and bulk tank samples of raw goat's milk on a BactoScan FC laser flow cytometer and at the same time using the anchor method according to STN EN ISO 4833-1 and after their subsequent logarithmic transformation, such a conversion was created in the form of log10(CFU/mL) = 1.0174 x log10(IBC/ml) + 2.7483. Subsequently, this was verified by comparing the recalculated and directly measured results in CFU/mL, using additional data set of samples. The influence of the somatic cell count in raw goat's milk, as a possible interfering factor, was also assessed.
山羊奶及其产品在斯洛伐克越来越受欢迎。生羊奶的强制性健康安全指标之一是细菌总数。细菌总数可以通过锚平板培养法测定，但这种方法既繁琐又费力。激光流式细胞仪是测量生鲜乳微生物质量的现代替代方法。为了将激光流式细胞仪法得出的结果用于立法目的，有必要将该方法得出的主要测量结果转换成具有代表性的 CFU/mL 标度。这项工作介绍了如何将生鲜羊奶转换成适用于斯洛伐克地区的标准。在 BactoScan FC 激光流式细胞仪上测量了数百个个体和散装罐装生羊奶样品，同时使用 STN EN ISO 4833-1 规定的锚定方法，并对其进行对数转换后，得出了 log10（CFU/mL）= 1.0174 x log10（IBC/ml）+ 2.7483 的转换结果。随后，使用额外的样本数据集，通过比较以 CFU/ml 为单位的重新计算结果和直接测量结果，验证了这一点。此外，还评估了生羊奶中体细胞数作为可能干扰因素的影响。
{"title":"MEASUREMENT OF MICROBIOLOGICAL QUALITY OF RAW GOAT´S MILK BY LASER FLOW CYTOMETRY","authors":"Martin Tomáška, Maroš Drončovský, Miroslav Kološta","doi":"10.55251/jmbfs.11014","DOIUrl":"https://doi.org/10.55251/jmbfs.11014","url":null,"abstract":"The popularity of goat's milk and its products is growing in Slovakia. Among the mandatory health safety features of raw goat's milk is the total bacterial count. It can be determined by the anchor plate-cultivation method, which is, however, tedious, and laborious. Laser flow cytometry is a modern alternative method for measuring the microbiological quality of raw milk. To use the results from laser flow cytometry method for legislative purposes, it is necessary to create a representative conversion of the primarily measured results obtained by this method into the CFU/mL scale. This work describes the creation of such a conversion for raw goat's milk, which will be valid for the Slovak region. After measuring several hundred individual and bulk tank samples of raw goat's milk on a BactoScan FC laser flow cytometer and at the same time using the anchor method according to STN EN ISO 4833-1 and after their subsequent logarithmic transformation, such a conversion was created in the form of log10(CFU/mL) = 1.0174 x log10(IBC/ml) + 2.7483. Subsequently, this was verified by comparing the recalculated and directly measured results in CFU/mL, using additional data set of samples. The influence of the somatic cell count in raw goat's milk, as a possible interfering factor, was also assessed.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141109165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kateryna Vanivska, Lucia Dianová, Marko Halo Jr., Nikola Štefunková, Michal Lenický, T. Slanina, Filip Tirpák, P. Ivanič, R. Stawarz, P. Massányi
The review focuses on research related to the effect of nanoparticles on cells in the animal and human body and on the analysis of potential biological effects that may be caused by exposure to these materials. This review deals with the mechanisms through which nanoparticles interact with cells and analyses their potential consequences for an individual's health. The main mechanisms of toxicity are oxidative stress, inflammatory responses, cell damage, and genetic changes that can lead to apoptosis or other cellular responses. These effects are often modified by various factors, including the size and shape of the nanoparticles, their surface treatment and chemical composition. The work highlights significant differences in the responses of cells of different organ systems to nanoparticles, thereby pointing the need for a more precise examination of toxic effects depending on the biological context. This review also discusses important methods for assessing nanoparticle toxicity, including in vitro experiments, in vivo animal studies, and various clinical studies. The aim of this review was to provide a comprehensive overview of this important issue, which has significant implications for public health and the environment. Research into nanoparticles and their interaction with cells is crucial for a better understanding the risks associated with their exposure and for the development of safe technologies and applications that use these materials.
{"title":"TOXICITY OF NANOPARTICLES ON ANIMAL AND HUMAN ORGANISM: CELL RESPONSE","authors":"Kateryna Vanivska, Lucia Dianová, Marko Halo Jr., Nikola Štefunková, Michal Lenický, T. Slanina, Filip Tirpák, P. Ivanič, R. Stawarz, P. Massányi","doi":"10.55251/jmbfs.10844","DOIUrl":"https://doi.org/10.55251/jmbfs.10844","url":null,"abstract":"The review focuses on research related to the effect of nanoparticles on cells in the animal and human body and on the analysis of potential biological effects that may be caused by exposure to these materials. This review deals with the mechanisms through which nanoparticles interact with cells and analyses their potential consequences for an individual's health. The main mechanisms of toxicity are oxidative stress, inflammatory responses, cell damage, and genetic changes that can lead to apoptosis or other cellular responses. These effects are often modified by various factors, including the size and shape of the nanoparticles, their surface treatment and chemical composition. The work highlights significant differences in the responses of cells of different organ systems to nanoparticles, thereby pointing the need for a more precise examination of toxic effects depending on the biological context. This review also discusses important methods for assessing nanoparticle toxicity, including in vitro experiments, in vivo animal studies, and various clinical studies. The aim of this review was to provide a comprehensive overview of this important issue, which has significant implications for public health and the environment. Research into nanoparticles and their interaction with cells is crucial for a better understanding the risks associated with their exposure and for the development of safe technologies and applications that use these materials.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141128958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natália Čeryová, Judita Lidiková, A. Bobková, M. Bobko, Monika Ňorbová, Silvia Fedorková, M. Orsák, Tomáš Rábek
Leek (Allium porrum L.), a commonly grown vegetable widely used in the food industry, has gained increased interest in recent years in connection with its content of bioactive substances. Therefore, this study aimed to determine and evaluate the influence of cultivar and morphological part on the total content of polyphenols, sulfur compounds, and the antioxidant activity in the monitored morphological parts of leek (Allium porrum L.). The total polyphenol content (TPC) in the white part of analyzed leek cultivars ranged from 446.30 to 1580.55 mg GAE.kg-1 DM. TPC in the green part of analyzed leek cultivars ranged from 748.09 to 2172.78 mg GAE.kg-1 DM. TPC in the leaves of analyzed leek cultivars ranged from 2301.24 to 5477.73 mg GAE.kg-1 DM. The antioxidant activity (AA) in the white part of analyzed leek cultivars ranged from 3.70 to 14.23 mmol TE.kg-1 DM. AA in the green part of analyzed leek cultivars ranged from 6.89 to 14.38 mmol TE.kg-1 DM. AA in leaves of analyzed leek cultivars ranged from 30.85 to 106.92 mmol TE.kg-1 DM. The total sulfur content (TSC) in the white part of analyzed leek cultivars ranged from 0.25 to 0.52 %. TSC in the green part of analyzed leek cultivars ranged from 0.27 to 0.52 %. TSC in the leaves of analyzed leek cultivars ranged from 0.31 to 0.59 %. Based on the analysis of variance, we can state that statistically higher (p< 0.05) TPC, AA, and TSC were determined in the leaves of the leek. Statistically higher (p< 0.05) TPC, AA, and TSC in all morphological parts were determined in the cultivar De Carentan. The results confirmed the variability of bioactive substances and antioxidant activity in the individual monitored cultivars, but also in the monitored morphological parts of the leek.
{"title":"VARIABILITY OF BIOACTIVE COMPOUNDS IN LEEK (ALLIUM PORRUM L.)","authors":"Natália Čeryová, Judita Lidiková, A. Bobková, M. Bobko, Monika Ňorbová, Silvia Fedorková, M. Orsák, Tomáš Rábek","doi":"10.55251/jmbfs.10638","DOIUrl":"https://doi.org/10.55251/jmbfs.10638","url":null,"abstract":"Leek (Allium porrum L.), a commonly grown vegetable widely used in the food industry, has gained increased interest in recent years in connection with its content of bioactive substances. Therefore, this study aimed to determine and evaluate the influence of cultivar and morphological part on the total content of polyphenols, sulfur compounds, and the antioxidant activity in the monitored morphological parts of leek (Allium porrum L.). The total polyphenol content (TPC) in the white part of analyzed leek cultivars ranged from 446.30 to 1580.55 mg GAE.kg-1 DM. TPC in the green part of analyzed leek cultivars ranged from 748.09 to 2172.78 mg GAE.kg-1 DM. TPC in the leaves of analyzed leek cultivars ranged from 2301.24 to 5477.73 mg GAE.kg-1 DM. The antioxidant activity (AA) in the white part of analyzed leek cultivars ranged from 3.70 to 14.23 mmol TE.kg-1 DM. AA in the green part of analyzed leek cultivars ranged from 6.89 to 14.38 mmol TE.kg-1 DM. AA in leaves of analyzed leek cultivars ranged from 30.85 to 106.92 mmol TE.kg-1 DM. The total sulfur content (TSC) in the white part of analyzed leek cultivars ranged from 0.25 to 0.52 %. TSC in the green part of analyzed leek cultivars ranged from 0.27 to 0.52 %. TSC in the leaves of analyzed leek cultivars ranged from 0.31 to 0.59 %. Based on the analysis of variance, we can state that statistically higher (p< 0.05) TPC, AA, and TSC were determined in the leaves of the leek. Statistically higher (p< 0.05) TPC, AA, and TSC in all morphological parts were determined in the cultivar De Carentan. The results confirmed the variability of bioactive substances and antioxidant activity in the individual monitored cultivars, but also in the monitored morphological parts of the leek.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140687815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katarína Poláková, A. Bobková, Alžbeta Demianová, M. Bobko, Lukáš Jurčaga, Andrea Mesárošová, Jozef Čapla, Ivana Timoracká, Judita Lidiková, Natália Čeryová
This review focuses on food fraud and shows a picture of the most critical notifications reported by the Knowledge Centre for Food Fraud and Quality worldwide during the year 2023. Knowledge4Policy (K4P) is the EU Commission's platform for evidence-based policymaking. This study aims to provide an overview of the cases of food fraud focused on specific food groups - (fish and seafood, oils and fats, fruit and vegetables, honey and sugar, meat and meat products, wine, alcohol, milk and dairy products, spices and herbs, grain-based food – cereals - bakery products, eggs, snacks, coffee, tea, cocoa, non-alcoholic beverages, dietic foods supplements, fortified food). The data on food fraud was divided based on world continents (Asia, Africa, South America, North- Central America, Australia, and Europe). The next part of our overview shows the count of food fraud incidents separately each month for 2023. Overall, 419 cases were registered worldwide in 66 countries in 2023. Most cases were registered in Europe, South America, Africa, North-Central America, and Australia. Based on our findings, we can conclude that the highest number of cases were registered in Pakistan, followed by Italy, Brazil, Nigeria, the Dominican Republic, and Australia. The most abundant was alcohol-beverages adulteration (18.13 % of the total number of cases reported in 2023 worldwide), followed by meat and meat products (15.03 %), fish and seafood (13.60 %), and grain-based food, cereals, and bakery products (11.69 %). Analysis of notification data showed that the most used type of fraud was grey market trading, mislabeling, document forgery, dilution/mixing, substitution, and unapproved enhancement. Food fraud databases serve as valuable tools for the food industry and regulatory bodies, aiding in mitigating food fraud risks.
{"title":"ADULTERATION IN FOOD INDUSTRY IN 2023 - OVERVIEW","authors":"Katarína Poláková, A. Bobková, Alžbeta Demianová, M. Bobko, Lukáš Jurčaga, Andrea Mesárošová, Jozef Čapla, Ivana Timoracká, Judita Lidiková, Natália Čeryová","doi":"10.55251/jmbfs.11048","DOIUrl":"https://doi.org/10.55251/jmbfs.11048","url":null,"abstract":"This review focuses on food fraud and shows a picture of the most critical notifications reported by the Knowledge Centre for Food Fraud and Quality worldwide during the year 2023. Knowledge4Policy (K4P) is the EU Commission's platform for evidence-based policymaking. This study aims to provide an overview of the cases of food fraud focused on specific food groups - (fish and seafood, oils and fats, fruit and vegetables, honey and sugar, meat and meat products, wine, alcohol, milk and dairy products, spices and herbs, grain-based food – cereals - bakery products, eggs, snacks, coffee, tea, cocoa, non-alcoholic beverages, dietic foods supplements, fortified food). The data on food fraud was divided based on world continents (Asia, Africa, South America, North- Central America, Australia, and Europe). The next part of our overview shows the count of food fraud incidents separately each month for 2023. Overall, 419 cases were registered worldwide in 66 countries in 2023. Most cases were registered in Europe, South America, Africa, North-Central America, and Australia. Based on our findings, we can conclude that the highest number of cases were registered in Pakistan, followed by Italy, Brazil, Nigeria, the Dominican Republic, and Australia. The most abundant was alcohol-beverages adulteration (18.13 % of the total number of cases reported in 2023 worldwide), followed by meat and meat products (15.03 %), fish and seafood (13.60 %), and grain-based food, cereals, and bakery products (11.69 %). Analysis of notification data showed that the most used type of fraud was grey market trading, mislabeling, document forgery, dilution/mixing, substitution, and unapproved enhancement. Food fraud databases serve as valuable tools for the food industry and regulatory bodies, aiding in mitigating food fraud risks.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140689049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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