Association Between Cytochrome 1B1*3 Polymorphism and the Breast Cancer in a Group of Iranian Women

Abstract

Background: Asahormone-dependentcancer,estrogenisinvolvedinthedevelopmentof breastcancer. CYP1B1 belongstotheP450 superfamily of enzymes and is involved in the metabolism of estrogen. The present study investigates the relationship between CYP1B1*3 rs1056836 polymorphism and breast cancer in Iranian women. Methods: Thepresentcase-controlstudywasconductedon79womenwithbreastcancerand79healthywomenadmittedtoShoha-daye Tajrish hospital in Tehran. Blood samples were taken from all the participants and their leukocyte DNA was extracted. The PCR-RFLP method was used for genotyping the participants based on the size of the pieces on the gel. Based on Hardy-Weinberg equilibrium model, the frequency of alleles was calculated. Results: The mean age of participants was 48 ± 8 years old in the cancer group and 43 ± 6 years old in the control group. After counting the genotypes, their percentages were calculated as 30.38% for the GG genotype, 37.97% for the GC/CG and 31.65% for the CC in the cancer group and as 32.91% for the GG genotype, 53.16% for the GC/CG and 13.93% for the CC in the control group. Based on Hardy-Weinberg equilibrium model, the frequency of the G allele and C allele was 49.37% and 50.63 in the cancer group, and about 59.49% and 40.51% in the control group. A statistically significant difference was observed between the two groups in terms of the CC homozygotes (P = 0.008). Conclusions: Theresultsobtainedshowedpossibilityof asignificantrelationshipbetween CYP1B1 rs1056836polymorphismandthe riskof developingbreastcancer,andthepolymorphismcan,therefore,besaidtobeinvolvedinthedevelopmentof thiscondition.