In-vitro and In-silico Inhibitory Action of Methanol Extract of Rhynchosia beddomei Whole Plant on α-Glucosidase and α-Amylase

Abstract

The goal of the current study was to identify potential bioactive components that may be responsible for Rhynchosia beddomei's antidiabetic activity through in silico docking study and to investigate potential mechanisms by which Rhynchosia beddomei may be helpful in managing diabetes and its associated complications.  In vitro anti-diabetic assays showed that this plant's high efficiency to inhibit α-amylase (62.13%) and α-glucosidase (59.9%) enzymatic activity, which are well-established targets for the management of diabetes, is responsible for its anti-hyperglycaemic activity. Soxhlet extraction produced an extractive yield of 35.43%.  Additionally, we hypothesised that flavonoids including rutin, lucenin, orientin, rhynchosin, and isooreintin contained in this plant may be accountable for the antidiabetic characteristics through docking experiments. The traditional usage of this herb as an antidiabetic is supported scientifically by these findings.  By reducing dietary glucose intake, it may help slow the course of diabetic complications and control diabetes. The therapy of hyperglycaemia requires further screening of predicted antidiabetic compounds.