Laboratory and Field Susceptibility of Drosophila suzukii Matsumura (Diptera: Drosophilidae) to Entomopathogenic Fungal Mycoses1

Q2 Agricultural and Biological Sciences The Journal of Agricultural and Urban Entomology Pub Date : 2017-11-29 DOI:10.3954/1523-5475-33.1.111
Gabriel Alnajjar, F. Drummond, E. Groden
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引用次数: 6

Abstract

Abstract Spotted wing drosophila (Drosophila suzukii Matsumura; Diptera: Drosophilidae) is a recently established invasive insect pest of berries and stone fruits. We tested the susceptibility of adult D. suzukii to infection by four species of entomopathogenic fungi: Beauveria bassiana (Bals.) Vuill., strains GHA and HF-23; Isaria fumosorosea Wize, strains FE-9901 and Apopka 97; Metarhizium anisopliae var anisopliae (Metschn.), strain F-52; and Metarhizium robertsii, strain DW-346; in laboratory bioassays. Scanning electron micrographs confirmed germination of conidia on D. suzukii integument. Exposure to conidia of all fungal isolates significantly increased mortality rates in comparison to untreated flies. In a subsequent bioassay of the two most virulent isolates, increasing pathogen dose from 0–16,000 conidia mm−2 of B. bassiana strain GHA increased fly mortality and proportion of sporulating cadavers. While fly inoculations with M. anisopliae at 0–4000 conidia mm−2 did not yield any measurable mortality response, a positive correlation was observed between dose and frequency of sporulating cadavers. In addition, we found that oocyte maturation rates were curtailed through one week of adulthood development after sub-lethal exposure to B. bassiana conidia. Despite promising laboratory results, a field cage experiment demonstrated that application of B. bassiana did not protect fruit from infestation. Taken together, these results show that entomopathogenic fungi are virulent against D. suzukii in the laboratory, but application in the field did not show promise.
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松村铃木果蝇(双翅目:果蝇科)对昆虫病原真菌真菌的室内和田间敏感性研究[j]
斑点翅果蝇(drosophila suzuki Matsumura;双翅目:果蝇科)是一种新发现的侵害浆果和核果的害虫。研究了成年猪嗜血杆菌对球孢白僵菌(Beauveria bassiana, Bals;Vuill。菌株GHA和HF-23;菌株FE-9901和Apopka 97;金龟子绿僵菌(Metschn.) F-52;罗伯特绿僵菌,菌株DW-346;在实验室生物分析中。扫描电镜证实了铃木被毛上分生孢子的萌发。与未经处理的苍蝇相比,暴露于所有真菌分离株的分生孢子显著增加了死亡率。在随后对两种毒性最强的分离株进行的生物测定中,从0-16,000分生孢子mm - 2中增加球孢白僵菌菌株GHA的病原体剂量会增加苍蝇死亡率和产孢尸体比例。虽然在0-4000分生孢子mm - 2范围内接种绿僵菌的苍蝇没有产生任何可测量的死亡率反应,但在孢子尸体的剂量和频率之间观察到正相关。此外,我们发现,在暴露于分生球孢白僵菌亚致死后,卵母细胞成熟率在一周的成年期发育中有所降低。尽管实验室结果令人鼓舞,但田间笼试验表明,球孢白僵菌的施用并不能保护果实免受侵染。综上所述,这些结果表明,昆虫病原真菌在实验室中对猪嗜血杆菌具有毒力,但在野外的应用前景并不乐观。
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来源期刊
The Journal of Agricultural and Urban Entomology
The Journal of Agricultural and Urban Entomology Agricultural and Biological Sciences-Insect Science
CiteScore
1.40
自引率
0.00%
发文量
2
期刊介绍: The Journal of Agricultural and Urban Entomology (JAUE) (Journal of Agricultural Entomology, Jan 1984 - Oct 1998 volumes 1-15) is published under the auspices of the South Carolina Entomological Society (SCES). The Journal publishes contributions of original research concerning insects and other arthropods of agricultural and urban importance to include those affecting humans, livestock, poultry, and wildlife. JAUE is particularly dedicated to the publication of articles and notes pertaining to applied entomology, although it will accept suitable contributions of a fundamental nature related to agricultural and urban entomology.
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