Starvation Induces Endoplasmic Reticulum Stress and Autophagy in Malpighian Tubules of Two-Spotted Field Crickets Gryllus bimaculatus

IF 0.2 4区 农林科学 Q4 ENTOMOLOGY Journal of the Kansas Entomological Society Pub Date : 2020-12-15 DOI:10.2317/0022-8567-93.1.88
Nuri Lee, Ji-Hye Song, Y. Ko, Kisang Kwon, Eun-Ryeong Lee, O. Kwon
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引用次数: 1

Abstract

ABSTRACT: We investigated gene expression related to endoplasmic reticulum (ER) stress, apoptosis, and autophagy to understand how Gryllus bimaculatus (two-spotted field cricket) overcomes starvation stress. Male adults were used in starvation experiments. Expression levels of genes related to ER stress, apoptosis, and autophagy were investigated for each tissue after starvation stress (consisting of 1, 3, and 6 days of starvation, and 1 and 2 days of refeeding after 6 days of starvation) by reverse-transcription PCR. Furthermore, Malpighian tubules were stained with LysoTracker Red to observe autophagy. Starvation significantly upregulated gene expression of ER chaperones in the Malpighian tubules of G. bimaculatus. Gene expression levels of BiP, ERap2, and SERCA were upregulated up to 2.5-fold, ATF6 was upregulated up to 2-fold, and PDI and Derl2 were upregulated up to 1.5-fold in the starvation group compared to controls. Although autophagy was induced by starvation, apoptosis was not. Notably, gene expression of ATG9 was upregulated up to 15-fold in the Malpighian tubules of G. bimaculatus, which is the highest among the autophagy-related genes. These results suggest that ER stress-induced autophagy appears to be acutely involved in the mechanism by which insects overcome starvation stress.
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饥饿诱导双斑蟋蟀Malpighian微管内质网应激和自噬
摘要:我们研究了与内质网应激、细胞凋亡和自噬相关的基因表达,以了解双斑蟋蟀如何克服饥饿应激。成年男性被用于饥饿实验。通过逆转录聚合酶链式反应研究饥饿应激(包括饥饿1、3和6天,以及饥饿6天后再喂养1和2天)后每个组织的ER应激、细胞凋亡和自噬相关基因的表达水平。此外,用LysoTracker红染色Malpighian小管以观察自噬。饥饿显著上调了双斑蝥马尔皮氏管中ER伴侣基因的表达。与对照组相比,饥饿组中BiP、ERap2和SERCA的基因表达水平上调高达2.5倍,ATF6上调高达2倍,PDI和Derl2上调高达1.5倍。尽管自噬是由饥饿诱导的,但细胞凋亡不是。值得注意的是,ATG9的基因表达在双斑G.bimaculatus的Malpighian小管中上调了15倍,这是自噬相关基因中最高的。这些结果表明,内质网应激诱导的自噬似乎与昆虫克服饥饿应激的机制密切相关。
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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
12
审稿时长
>12 weeks
期刊介绍: The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.
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