Assessing oxygen limiting fermentation conditions for 2,3-butanediol production from Paenibacillus polymyxa

IF 2.5 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Frontiers in chemical engineering Pub Date : 2022-11-04 DOI:10.3389/fceng.2022.1038311
Ryan J. Stoklosa, R. Latona, D. Johnston
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引用次数: 1

Abstract

2,3-butanediol (2,3-BDO) is a platform chemical that can be converted to a wide array of products ranging from bio-based materials to sustainable aviation fuel. This chemical can be produced by a variety of microorganisms in fermentation processes. Challenges remain for high titer 2,3-BDO production during fermentation due to several parameters, but controlling oxygen is one of the most relevant processing parameters to ensure viable product output. This work investigated the fermentation of plant biomass sugars by the 2,3-BDO producer Paenibacillus polymyxa. Aerobic and oxygen limited fermentation conditions were initially evaluated using molasses-based media to determine cell growth and 2,3-BDO output. Similar conditions were then evaluated on hydrolysate from pretreated sweet sorghum bagasse (SSB) that contained fermentable sugars from structural polysaccharides. Fermentations in molasses media under aerobic conditions found that 2,3-BDO could be generated, but over time the amount of 2,3-BDO decreased due to conversion back into acetoin. Oxygen limited fermentation conditions exhibited improved biomass growth, but only limited suppression of 2,3-BDO conversion to acetoin occurred. Glucose depletion appeared to have a greater role influencing 2,3-BDO conversion back into acetoin. Further improvements in 2,3-BDO yields were found by utilizing detoxified SSB hydrolysate.
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多粘菌Paenibacillus polymyxa生产2,3-丁二醇的限氧发酵条件评估
2,3-丁二醇(2,3- bdo)是一种平台化学品,可以转化为一系列产品,从生物基材料到可持续航空燃料。这种化学物质可以由多种微生物在发酵过程中产生。在发酵过程中,由于几个参数的影响,高滴度2,3- bdo的生产仍然存在挑战,但控制氧气是确保可行产品产出的最相关的加工参数之一。本文研究了2,3- bdo产生物多粘类芽孢杆菌对植物生物质糖的发酵。用糖蜜为基础的培养基初步评估了有氧和限氧发酵条件,以确定细胞生长和2,3- bdo的产量。然后对含有结构多糖可发酵糖的预处理甜高粱甘蔗渣(SSB)的水解产物进行了类似的条件评价。在好氧条件下的糖蜜培养基中发酵发现可以生成2,3- bdo,但随着时间的推移,2,3- bdo的量由于转化回乙酰而减少。限氧发酵条件下,生物质生长得到改善,但对2,3- bdo转化为乙酰氨基酚的抑制作用有限。葡萄糖消耗似乎对2,3- bdo转化回乙酰氨基酚有更大的影响。利用解毒的SSB水解液进一步提高了2,3- bdo的产量。
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CiteScore
3.50
自引率
0.00%
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审稿时长
13 weeks
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