Cytotoxicity and penetration enhancement activity of essential oils from warming the interior medicinals with hot or warm property in terms of Traditional Chinese Medicine

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-04-01 DOI:10.1016/j.jtcm.2018.03.006
Yao Junhong , Jiang Qiudong , Cai Hao , Zhu Xuemin , Ma Min , Duan Jinao , Dong Jie , Chen Jun
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引用次数: 3

Abstract

OBJECTIVE

To investigate on the cytotoxicity and penetration enhancement effect of essential oils (EOs) from warming the interior medicinals (WIM) from Traditional Chinese Medicine (TCM).

METHODS

EOs were extracted from WIM of Bichengqie (Litseae Fructus), Dingxiang (Flos Syzygii Aromatici), Huajiao (Pericarpium Zanthoxyli Bungeani), and Xiaohuixiang (Fructus Foeniculi) with warm nature, and Ganjiang (Rhizoma Zingiberis), Gaoliangjiang (Rhizoma Alpiniae Officinari), Rougui (Cortex Cinnamomi Cassiae), and Wuzhuyu (Fructus Evodiae Rutaecarpae) with hot nature, respectively. Their chemical compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxicity of the extracted eight EOs on HaCaT cells was measured and compared. Moreover, analyses of cell cycle and cell apoptosis were performed to investigate the cytotoxic mechanism. The transdermal penetration enhancement effects of the extracted eight EOs on ibuprofen were further compared by the modified Franz diffusion cell method.

RESULTS

The most abundant constituents in the extracted eight EOs were determined to be monoterpenes, especially oxygen-containing monoterpenes. The HaCaT cell cytotoxicity of EOs from WIM with hot nature were significantly (P = 0.020) higher than that with warm nature. Both ginger oil and zanthoxylum oil significantly induced G0/G1 phase arrestment in HaCaT cell cycle. For ginger oil from WIM with hot nature and zanthoxylum oil from WIM with warm nature, the main mechanisms of the cytotoxicity were found to be the induction of cellular necrosis and the cellular apoptosis, respectively. Furthermore, most of the tested EOs showed remarkable penetration enhancement activity on ibuprofen. However, no statistical significance (P = 0.18) was found between penetration enhancement activity of EOs from WIM with warm nature and EOs from WIM with hot nature.

CONCLUSION

With the enhanced penetration activity, the extracted EOs from the WIM demonstrated their significant effect of the cytotoxicity on the skin cells.

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中药温性内药精油的细胞毒性及增透活性
目的研究中药温内精油的细胞毒性和增透作用。方法分别从温性药材碧城旗、丁香、花椒、小慧香和热性药材甘姜、高梁姜、桂皮、乌竹玉中提取皂苷类化合物。采用气相色谱-质谱联用(GC-MS)对其化学成分进行分析。测定并比较提取的8种EOs对HaCaT细胞的细胞毒性。此外,通过细胞周期和细胞凋亡分析来探讨细胞毒性机制。采用改良的Franz扩散池法比较提取的8种EOs对布洛芬的透皮增强作用。结果8种精油中含量最高的成分均为单萜,含氧单萜含量最高。热性WIM中EOs的HaCaT细胞毒性显著高于温性WIM (P = 0.020)。姜油和花椒油均能显著诱导HaCaT细胞周期G0/G1期阻滞。热性姜油和温性姜油的细胞毒性作用机制主要为诱导细胞坏死和细胞凋亡。此外,大多数测试的EOs对布洛芬具有显著的渗透增强活性。而温性与热性两种药的EOs增强穿透活性差异无统计学意义(P = 0.18)。结论黄芪提取物对皮肤细胞具有明显的细胞毒作用,渗透活性增强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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