The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours

S. Bintara, D. T. Widayati, W. Asmarawati, R. N. Aji
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Abstract

In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls.
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番茄红素添加量对5℃保存24 h的沙嫩山羊精液质量的影响
为了增加牲畜数量,需要一个良好的繁殖过程。番茄红素属于类胡萝卜素类,具有暗黄色色素。本研究旨在测定添加番茄红素对萨能山羊精液质量的影响。研究设计使用了4种处理,P0(蛋黄三稀释剂)作为对照,P1(蛋黄三稀释液+2%番茄红素),P2(蛋黄三浓缩液+4%番茄红素)和P3(蛋黄三混合稀释剂)。鸡蛋+6%番茄红素)。精液在5℃下保存24小时后,观察精液质量。宏观数据进行描述性分析,微观数据采用完全随机设计(CRD)进行分析。结果表明,蛋黄三稀释液中添加番茄红素对萨能山羊精液质量有显著影响(P<0.05)。结果显示,各处理组(P0、P1、P2和P3)精子活力分别为60.1±5.7%、65.2±4.3%、62.4±5.2%和60.6±4.7%,活力分别为65.3±4.8、73.5±4.2、70.4±3.8和66.1±5.1,异常分别为17±1.8%、16±1.8%、18±1.9%和16±1.7%,膜完整性分别为63±3.3%、70±3.6%,分别为67±3.2%和62±2.9%。根据研究结果,可以得出结论,与对照组相比,番茄红素的添加改善了萨能山羊的精液质量。
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