Induced polyploidization in buckwheat (Fagopyrum esculentum Moench)

IF 1 4区 生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2022-09-30 DOI:10.31742/isgpb.82.3.8
A. Srivastava, G. Kumar
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Abstract

The significance of polyploidy in the evolutionary process of plants history is well documented. In this concern, seed priming with colchicine as well as the cotton-swab method for polyploidy induction in plants becomes much acquainted in cytogenetic study. The aim of present study was to achieve chromosomal doubling by the application of colchicine to apical meristem of young seedlings. The young seedlings were treated with three different concentrations of colchicine (0.2, 0.4 and 0.6%) for durations of 12, 24 and 36 h each. The colchiploidy with highest proportion approximately 50% with 2n=4x=32 was found at 0.2% concentration for 24 and 36 h. Autotetraploid enduring plants were identified with morphological and cytological variations such as thickness of leaf, larger stomata with low density, larger pollen, large flower etc. Buds were selected from these plants for the cytological study. Cytological analysis including chromosome counting demonstrated that the chromosome number was doubled. The confirmation of autotetraploid plants was achieved by this technique. Tetraploid plants were grown up to maturity and the harvested seeds were sown to establish the second generation which may be used in future breeding programme
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荞麦的诱导多倍体化
多倍体在植物进化过程中的重要意义是有案可查的。因此,在细胞遗传学研究中,秋水仙碱引种法和棉签法诱导植物多倍体已为人们所熟知。本研究的目的是利用秋水仙碱在幼苗顶端分生组织中的应用,实现染色体加倍。幼苗分别用浓度为0.2、0.4和0.6%的秋水仙碱处理12、24和36 h。在0.2%浓度处理24和36 h时,可获得最高比例约为50% (2n=4x=32)的同源四倍体耐久植株,其形态和细胞学变化表现为叶片厚度大、气孔大但密度低、花粉大、花大等。从这些植物中选取芽进行细胞学研究。包括染色体计数在内的细胞学分析表明染色体数目增加了一倍。利用该技术,获得了同源四倍体植株。将四倍体植株培育至成熟,并将收获的种子播种以建立可用于未来育种计划的第二代植株
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来源期刊
CiteScore
1.80
自引率
10.00%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
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