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{"title":"Laboratory Cultivation and Storage of Shigella","authors":"Shelley M. Payne","doi":"10.1002/cpmc.93","DOIUrl":null,"url":null,"abstract":"<p><i>Shigella</i> species, which are closely related to <i>Escherichia coli</i>, can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and for monitoring of the presence of the virulence plasmid. © 2019 by John Wiley & Sons, Inc.</p><p><b>Basic Protocol 1</b>: Growth of <i>S. flexneri</i> from frozen stocks or agar stabs</p><p><b>Basic Protocol 2</b>: Growth of <i>S. flexneri</i> in rich liquid medium</p><p><b>Alternate Protocol 1</b>: Growth of <i>S. flexneri</i> in rich defined medium</p><p><b>Alternate Protocol 2</b>: Growth of <i>S. flexneri</i> in minimal medium</p><p><b>Basic Protocol 3</b>: Storage of <i>S. flexneri</i> in frozen stocks</p><p><b>Alternate Protocol 3</b>: Storage of <i>S. flexneri</i> in agar stabs</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":"55 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.93","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.93","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Shigella species, which are closely related to Escherichia coli , can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and for monitoring of the presence of the virulence plasmid. © 2019 by John Wiley & Sons, Inc.
Basic Protocol 1 : Growth of S. flexneri from frozen stocks or agar stabs
Basic Protocol 2 : Growth of S. flexneri in rich liquid medium
Alternate Protocol 1 : Growth of S. flexneri in rich defined medium
Alternate Protocol 2 : Growth of S. flexneri in minimal medium
Basic Protocol 3 : Storage of S. flexneri in frozen stocks
Alternate Protocol 3 : Storage of S. flexneri in agar stabs
志贺氏菌的实验室培养与贮藏
志贺氏菌与大肠杆菌密切相关，易于在实验室中维持和储存。本文包括常规生长条件和培养基的制备、细菌的储存和毒力质粒存在的监测。©2019 by John Wiley &基本方案1:flexneri在冷冻培养基或琼脂培养基中生长基本方案2:flexneri在丰富液体培养基中生长备用方案1:flexneri在丰富液体培养基中生长备用方案2:flexneri在最小培养基中生长基本方案3:flexneri在冷冻培养基中储存备用方案3:flexneri在琼脂培养基中储存
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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