Immobilization of BMP-2-derived peptides on 3D-printed porous scaffolds for enhanced osteogenesis

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL Biomedical materials Pub Date : 2019-11-15 DOI:10.1088/1748-605X/ab4c78

Xiashiyao Zhang, Qi Lou, Lili Wang, S. Min, Meng Zhao, Changyun Quan

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引用次数: 12

Abstract

Three-dimensional (3D) printing technologies open up new perspectives for customizing the external shape and internal architecture of bone scaffolds. In this study, an oligopeptide (SSVPT, Ser-Ser-Val-Pro-Thr) derived from bone morphogenetic protein 2 was conjugated with a dopamine coating on a 3D-printed poly(lactic acid) (PLA) scaffold to enhance osteogenesis. Cell experiments in vitro showed that the scaffold was highly osteoconductive to the adhesion and proliferation of rat marrow mesenchymal stem cells (MSCs). In addition, RT-PCR analysis showed that the scaffold was able to promote the expression of osteogenesis-related genes, such as alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN) and osteopontin (OPN). Images of the micro-CT 3D reconstruction from the rat cranial bone defect model showed that bone regeneration patterns occurred from one side edge towards the center of the area implanted with the prepared biomimetic peptide hydrogels, demonstrating significantly accelerated bone regeneration. This work will provide a basis to explore the application potential of bioactive scaffolds further.

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bmp -2衍生肽在3d打印多孔支架上的固定化促进成骨

三维（3D）打印技术为定制骨支架的外部形状和内部结构开辟了新的视角。在本研究中，将源自骨形态发生蛋白2的寡肽（SSVPT，Ser-Ser-Val-Pro-Thr）与3D打印的聚乳酸（PLA）支架上的多巴胺涂层偶联，以增强成骨作用。体外细胞实验表明，该支架对大鼠骨髓间充质干细胞（MSC）的粘附和增殖具有良好的骨传导性。此外，RT-PCR分析表明，该支架能够促进成骨相关基因的表达，如碱性磷酸酶（ALP）、runt相关转录因子2（RUNX2）、骨钙素（OCN）和骨桥蛋白（OPN）。大鼠颅骨缺损模型的显微CT 3D重建图像显示，骨再生模式从一侧边缘向植入所制备的仿生肽水凝胶的区域中心发生，表明骨再生显著加速。这项工作将为进一步探索生物活性支架的应用潜力提供基础。

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来源期刊

Biomedical materials 工程技术-材料科学：生物材料

CiteScore

6.70

自引率

7.50%

发文量

294

审稿时长

3 months

期刊介绍： The goal of the journal is to publish original research findings and critical reviews that contribute to our knowledge about the composition, properties, and performance of materials for all applications relevant to human healthcare. Typical areas of interest include (but are not limited to): -Synthesis/characterization of biomedical materials- Nature-inspired synthesis/biomineralization of biomedical materials- In vitro/in vivo performance of biomedical materials- Biofabrication technologies/applications: 3D bioprinting, bioink development, bioassembly & biopatterning- Microfluidic systems (including disease models): fabrication, testing & translational applications- Tissue engineering/regenerative medicine- Interaction of molecules/cells with materials- Effects of biomaterials on stem cell behaviour- Growth factors/genes/cells incorporated into biomedical materials- Biophysical cues/biocompatibility pathways in biomedical materials performance- Clinical applications of biomedical materials for cell therapies in disease (cancer etc)- Nanomedicine, nanotoxicology and nanopathology- Pharmacokinetic considerations in drug delivery systems- Risks of contrast media in imaging systems- Biosafety aspects of gene delivery agents- Preclinical and clinical performance of implantable biomedical materials- Translational and regulatory matters