The effect of ingested sulfite on active avoidance in normal and sulfite oxidase-deficient aged rats

IF 2.8 4区医学 Q2 TOXICOLOGY Toxicology Mechanisms and Methods Pub Date : 2017-02-01 DOI:10.1080/15376516.2016.1253812

O. Ozsoy, Sinem Aras, Ayse Ozkan, H. Parlak, B. Gemici, N. Uysal, M. Aslan, P. Yargiçoğlu,, A. Agar

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引用次数: 3

Abstract

Abstract The aim of this study was to investigate the possible toxic effects of sulfite on neurons by measuring active avoidance learning in normal and sulfite oxidase (SOX)-deficient aged rats. Twenty-four months of age Wistar rats were divided into four groups: control (C), sulfite-treated group (S), SOX-deficient group (D) and SOX-deficient + sulfite-treated group (DS). SOX deficiency was established by feeding rats with a low molybdenum (Mo) diet and adding 200 ppm tungsten (W) to their drinking water. Sulfite in the form of sodium metabisulfite (25 mg/kg) was given by gavage for six weeks. Active avoidance responses were determined by using an automated shuttle box. Hepatic SOX activity was measured to confirm SOX deficiency. The hippocampus was used for determining the activity of cyclooxygenase (COX) and caspase-3 enzymes and the level of prostaglandin E2 (PGE2) and nitrate/nitrite. SOX-deficient rats had an approximately 10-fold decrease in hepatic SOX activity compared with normal rats. Sulfite did not induce impairment of active avoidance learning in SOX-deficient rats and in normal rats compared with their control groups. Sulfite had no effect on the activity of COX and caspase-3 in the hippocampus. Treatment with sulfite did not significantly increase the level of PGE2 and nitrate/nitrite in the hippocampus.

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摄入亚硫酸盐对正常和亚硫酸氧化酶缺乏老年大鼠主动回避的影响

摘要本研究旨在通过测量正常和亚硫酸盐氧化酶(SOX)缺乏的老年大鼠的主动回避学习，探讨亚硫酸盐对神经元可能的毒性作用。24月龄Wistar大鼠分为4组:对照组(C)、亚硫酸盐处理组(S)、sox缺乏组(D)和sox缺乏+亚硫酸盐处理组(DS)。以低钼(Mo)饲料喂养大鼠，并在其饮水中添加200 ppm钨(W)，建立了SOX缺乏症。亚硝酸盐以焦亚硫酸钠形式(25 mg/kg)灌胃6周。主动回避反应由自动穿梭箱确定。测定肝脏SOX活性以证实SOX缺乏。采用海马组织检测大鼠环氧化酶(COX)和caspase-3酶活性、前列腺素E2 (PGE2)和硝酸盐/亚硝酸盐水平。与正常大鼠相比，SOX缺陷大鼠的肝脏SOX活性降低了大约10倍。与对照组相比，亚硫酸盐没有引起sox缺陷大鼠和正常大鼠主动回避学习的损害。亚硫酸盐对海马组织COX和caspase-3活性无影响。亚硫酸盐处理并没有显著增加海马中PGE2和硝酸盐/亚硝酸盐的水平。

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Toxicology Mechanisms and Methods TOXICOLOGY-

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3.10%

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期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.