Identification and validation of genetic locus linked to flavonoid and anthocyanin content in rice using Bulk Segregant Analysis

IF 1 4区生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2022-09-29 DOI:10.31742/isgpb.82.3.4

M. A. Fathima, S. Geetha, M. Hemalatha, K. Amudha

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Abstract

A study was undertaken to identify the genetic locus linked with the therapeutic traits, viz., flavonoid and anthocyanin contents in rice grains by Bulk Segregant Analysis using the F2 population derived from the cross, ADT(R)48/Kavuni. The donor for high anthocyanin and flavonoid content was Kavuni, one of the popular landraces of Tamil Nadu, which exists in various forms with respect to grain pigmentation. Through this study, three linked SSR markers, namely, RM228 (Chromosome 10) for flavonoid and anthocyanin content, RM5348 (Chromosome 10) for flavonoid content, and RM297 (Chromosome 1) for anthocyanin content, were identified. Among these markers, RM5348 was identified as a novel marker, lies in the genomic region of the gene encoding the enzyme flavonoid 3’-monooxygenase indicating more reliability. The association of these identified markers was further validated through Single Marker Analysis (SMA), and the results confirmed the strong association of these markers with the respective traits in various forms of Kavuni, other pigmented landraces, and improved cultivars. However, the validation must also be done in a population with another genetic background to prove the stern association of these markers with the traits.

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水稻类黄酮和花青素含量相关基因座的本体分离分析与验证

利用来自杂交后代ADT（R）48/Kavuni的F2群体，通过Bulk Segregant分析，确定了与治疗性状（即稻米中类黄酮和花青素含量）相关的遗传位点。高花青素和类黄酮含量的供体是Kavuni，它是泰米尔纳德邦流行的地方品种之一，在谷物色素沉着方面以各种形式存在。通过本研究，鉴定了三个连锁的SSR标记，即类黄酮和花青素含量的RM228（10号染色体）、类黄酮含量的RM5348（10号号染色体）和花青素内容的RM297（1号染色体）。在这些标记中，RM5348被鉴定为一个新的标记，位于类黄酮3'-单加氧酶编码基因的基因组区域，表明其可靠性更高。通过单标记分析（SMA）进一步验证了这些已鉴定标记的关联性，结果证实了这些标记与各种形式的卡武尼、其他有色地方品种和改良品种的各自性状的强关联性。然而，还必须在具有其他遗传背景的群体中进行验证，以证明这些标记与性状的严格关联。

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.