{"title":"Microwave-assisted citrate extraction (MaCE) as an alternative to autoclave citrate extraction (ACE) of a soil protein fraction","authors":"Qianyi Wu, K. Congreves, R. Farrell","doi":"10.1139/cjss-2023-0016","DOIUrl":null,"url":null,"abstract":"Soil protein is an important indicator of soil health and for soil health assessments is usually determined using autoclaved citrate extraction (ACE) followed by protein quantification using the Bradford or bicinchoninic acid (BCA) assay. Here we investigated an alternative extraction process using microwave-assisted citrate extraction (MaCE). We show that protein yield increases as the extraction time increases, but that yields comparable to those obtained using the standard ACE method can be obtained with an extraction time as short as 15 min. To the best of our knowledge, this is the first report of microwave-assisted extraction being used to determine this soil protein pool.","PeriodicalId":9384,"journal":{"name":"Canadian Journal of Soil Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian Journal of Soil Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1139/cjss-2023-0016","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"SOIL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Soil protein is an important indicator of soil health and for soil health assessments is usually determined using autoclaved citrate extraction (ACE) followed by protein quantification using the Bradford or bicinchoninic acid (BCA) assay. Here we investigated an alternative extraction process using microwave-assisted citrate extraction (MaCE). We show that protein yield increases as the extraction time increases, but that yields comparable to those obtained using the standard ACE method can be obtained with an extraction time as short as 15 min. To the best of our knowledge, this is the first report of microwave-assisted extraction being used to determine this soil protein pool.
期刊介绍：
The Canadian Journal of Soil Science is an international peer-reviewed journal published in cooperation with the Canadian Society of Soil Science. The journal publishes original research on the use, management, structure and development of soils and draws from the disciplines of soil science, agrometeorology, ecology, agricultural engineering, environmental science, hydrology, forestry, geology, geography and climatology. Research is published in a number of topic sections including: agrometeorology; ecology, biological processes and plant interactions; composition and chemical processes; physical processes and interfaces; genesis, landscape processes and relationships; contamination and environmental stewardship; and management for agricultural, forestry and urban uses.
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