Micropropagation of lavender: a protocol for production of plantlets

IF 0.7 4区农林科学 Q4 HORTICULTURE Horticultura Brasileira Pub Date : 2021-12-01 DOI:10.1590/s0102-0536-20210409

Jana Koefender, C. E. Manfio, J. N. Camera, André Schoffel, D. P. Golle

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引用次数: 6

Abstract

ABSTRACT Lavender (Lavandula dentata) has several medicinal applications, both in the fresh form and after industrial processing. Productivity increase could be obtained through the mass propagation, expanding the possibility of obtaining raw material for production of essential oil. The objective of this work was to develop a micropropagation protocol from explants obtained from seeds. Aspects of in vitro germination, multiplication, elongation and rooting were evaluated and, in sequence, the acclimatization process as well. The in vitro germination of lavender seeds can be obtained using a medium composed of agar and water. For multiplication, the use of MS medium (Murashige & Skoog, 1962) added with 5.0 µM 6-benzylaminopurine to the nutrient medium is the most appropriate. For elongation and rooting, the use of growth regulators is unnecessary. Acclimatization can be obtained directly by transferring the explant to plastic bags with substrate, kept in a greenhouse. It was possible to develop a protocol for the in vitro cultivation (establishment, multiplication, elongation, rooting and acclimatization) of lavender from explants of seed origin.

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薰衣草的微繁:一种生产植株的方案

薰衣草(Lavandula dentata)具有多种药用价值，无论是新鲜的还是经过工业加工的。通过大量繁殖可以提高生产力，扩大了获得生产精油原料的可能性。本工作的目的是建立一种从种子获得的外植体进行微繁的方法。对其体外萌发、增殖、伸长和生根等方面进行了评价，并依次对其驯化过程进行了评价。使用琼脂和水组成的培养基可以获得薰衣草种子的离体萌发。增殖时，使用MS培养基(Murashige & Skoog, 1962)在营养培养基中添加5.0µM 6-苄基氨基嘌呤是最合适的。对于伸长和生根，不需要使用生长调节剂。通过将外植体转移到有基质的塑料袋中，在温室中保存，可以直接驯化。有可能从种子来源的外植体中制定出一套薰衣草离体培养方案(建立、增殖、伸长、生根和驯化)。

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来源期刊

Horticultura Brasileira 农林科学-园艺

CiteScore

1.40

自引率

28.60%

发文量

审稿时长

4-8 weeks

期刊介绍： The journal Horticultura Brasileira, a quarterly journal, is the Official Publication of the Sociedade de Olericultura do Brasil. Its abbreviated title is Hortic. bras., and it should be used in bibliographies, footnotes, references and bibliographic strips.