Molecular and genetic study for detection of Helicobacter pylori virulence genes among patients with diffuse large B-Cell lymphoma

Abstract

Background: The bacteria Helicobacter pylori (Hp) has supposed to confirm an oncogenic consequence with the evolution of malignancy events in stomach, effectiveness of Hp consider a top risk factor of gastric-diffuse large B-cell lymphoma (DLBCL). Aims: the aim of this study was to observe the correlation between virulence factors, especially cytotoxin association gene A (CagA) and the progress of gastric DLBCL. Materials and Methods: Biopsy samples (50) involved in this study (28 were men, and 22 women, with a mean age up to 57.64 years), among gastric-DLBCL patients, with (25) control (were 14 men and 11 women) without DLBCL, tested at Medical City Teaching Hospital and Teaching Laboratories, study conducted from January 1st to September 30, 2022. Diagnosis was by molecular methods included DNA extraction for Hp by STR-Format kit (BIORON Diagnostics GmbHm In den Rauhweiden 20,67354 Germany), and “real-time polymerase chain reaction” methods using the (ABI PRISM 7700) Sequence Detection System, also applied the primers and probes, for Hp-CagA. Results: The mean of age was 55.7 ± 2 in DLBCL, while in control were 61.82 + 6, CagA +ve 66.66 + 2. In gastric DLBCL, the red blood cell count, white blood cell count, platelets count, and hemoglobin were all decline in DLBCL, not in the control group, lactate dehydrogenase levels in gastric DLBCL patients were rise (322 U/L), while less in control, while albumin (322g/dl) was less in gastric DLBCL patients. Hp 16S rRNA was detected relating to CagA variety, 66% (33/50) were CagA positive with highly significant in 23 patients with DLBCL (P = 0.004), and 10 in control patients. CagA gene expression was highly expression in gastric DLBCL patients with range up to (+), 8 (++), and 12 (+++), while in the control group was 5 (+), 2 (++), and 3 (+++), respectively. Conclusions: This present study fixed high frequency of CagA gene in gastric DLBCL patients. Moreover, confirmed this gene is significantly associated with gastric DLBCL in patients with Hp. Moreover, genotype EPIYA-ABC motif is related with the high risk of gastric DLBCL.