Patricia Isabel Torres Chavez, Leslie Denise Chaidez-Laguna, B. Ramírez-Wong, E. Márquez‐Ríos, A. Islas-Rubio, E. Carvajal‐Millan, J. Juárez-Onofre
{"title":"Impact of L-cysteine addition on nixtamalized corn dough: proteins and viscoelasticity","authors":"Patricia Isabel Torres Chavez, Leslie Denise Chaidez-Laguna, B. Ramírez-Wong, E. Márquez‐Ríos, A. Islas-Rubio, E. Carvajal‐Millan, J. Juárez-Onofre","doi":"10.18633/biotecnia.v24i1.1564","DOIUrl":null,"url":null,"abstract":"\nThe effect of L-cysteine on the solubility, protein structure, rheological and textural characteristics of corn masas were investigated. Masa was prepared with L-cysteine at two levels (0.25, 0.50 %). Size exclusion chromatography and Fourier transform infrared spectroscopy were utilized for protein characterization. Dynamic rheology and textural profile analysis were carried out on masas. There was a significant difference in relative solubility between the control and masas containing L-cysteine. Treatments with L-cysteine increased the relative solubility of proteins. Determination of FT-IR revealed that addition of L-cysteine did not affects secondary structure of protein. Data also showed significant differences in textural properties. Masas con L-cysteine improved the springiness, adhesiveness and cohesiveness. This investigation reveal that L-cysteine, may improve the elasticity or storage moduli, important characteristics of masas. \n","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":" ","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2022-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotecnia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18633/biotecnia.v24i1.1564","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The effect of L-cysteine on the solubility, protein structure, rheological and textural characteristics of corn masas were investigated. Masa was prepared with L-cysteine at two levels (0.25, 0.50 %). Size exclusion chromatography and Fourier transform infrared spectroscopy were utilized for protein characterization. Dynamic rheology and textural profile analysis were carried out on masas. There was a significant difference in relative solubility between the control and masas containing L-cysteine. Treatments with L-cysteine increased the relative solubility of proteins. Determination of FT-IR revealed that addition of L-cysteine did not affects secondary structure of protein. Data also showed significant differences in textural properties. Masas con L-cysteine improved the springiness, adhesiveness and cohesiveness. This investigation reveal that L-cysteine, may improve the elasticity or storage moduli, important characteristics of masas.
研究了l -半胱氨酸对玉米浆料溶解度、蛋白质结构、流变性和质地特性的影响。采用0.25、0.50%两种水平的l -半胱氨酸制备Masa。采用粒径排除色谱法和傅里叶变换红外光谱法对蛋白质进行表征。对masas进行了动态流变学和纹理分析。对照组和含l -半胱氨酸的masas的相对溶解度有显著差异。l -半胱氨酸增加了蛋白质的相对溶解度。FT-IR测定表明,加入l -半胱氨酸对蛋白质二级结构没有影响。数据还显示了纹理性能的显著差异。Masas con l -半胱氨酸提高了弹性、黏附性和内聚性。该研究表明,l -半胱氨酸可以改善massas的重要特性弹性或储存模量。