Pub Date : 2023-12-12DOI: 10.18633/biotecnia.v26i1.2109
Carlos Eduardo Aragon López, Erika Gabriela Palomares Resendiz, Sergio Cuevas tellechea, Javier Rolando Reyna Granados, Beatriz Arellano Reynoso, Miguel Angel Sánchez Castro, Jose Clemente Leyva Corona, Marcela Ivone Morales Pablos
Ovine Enzootic Abortion (OEA) is caused by Chlamydia abortus and has recently been recognized as endemic in Mexico. Due to the impact of OEA on intensive lamb production, it is necessary to establish a sanitary control plan based on an efficient diagnosis. The loop-mediated isothermal amplification technique (LAMP) is a molecular test that can specifically identify C. abortus. The aim of this study was to standardize the LAMP technique for the specific detection of C. abortus from ovine vaginal exudate. Primers were identified and modified from an electronic database. The effective conditions of the technique were determined using synthetic DNA and positive biological samples by PCR as references. The assay detection limit was 1 × 10-5 of genetic material, equivalent to 8.5 copies. The standardized LAMP test is proposed as a novel molecular tool for the rapid and specific diagnosis of C. abortus infection from ovine vaginal exudate samples in Mexico.
{"title":"Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants","authors":"Carlos Eduardo Aragon López, Erika Gabriela Palomares Resendiz, Sergio Cuevas tellechea, Javier Rolando Reyna Granados, Beatriz Arellano Reynoso, Miguel Angel Sánchez Castro, Jose Clemente Leyva Corona, Marcela Ivone Morales Pablos","doi":"10.18633/biotecnia.v26i1.2109","DOIUrl":"https://doi.org/10.18633/biotecnia.v26i1.2109","url":null,"abstract":"\u0000Ovine Enzootic Abortion (OEA) is caused by Chlamydia abortus and has recently been recognized as endemic in Mexico. Due to the impact of OEA on intensive lamb production, it is necessary to establish a sanitary control plan based on an efficient diagnosis. The loop-mediated isothermal amplification technique (LAMP) is a molecular test that can specifically identify C. abortus. The aim of this study was to standardize the LAMP technique for the specific detection of C. abortus from ovine vaginal exudate. Primers were identified and modified from an electronic database. The effective conditions of the technique were determined using synthetic DNA and positive biological samples by PCR as references. The assay detection limit was 1 × 10-5 of genetic material, equivalent to 8.5 copies. The standardized LAMP test is proposed as a novel molecular tool for the rapid and specific diagnosis of C. abortus infection from ovine vaginal exudate samples in Mexico.\u0000","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"52 18","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139007020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-11DOI: 10.18633/biotecnia.v26i1.2181
L. A. Ávila-Villa, G. A. Barco-Mendoza, R. Rodríguez-Ramirez, Jesús Daniel Villanueva‐Zayas, Marcel Martínez-Porchas, Alma Guadalupe Villa-Lerma, Belinda Vallejo-Córdoba, Josué Delgado-Domínguez
Shrimp aquaculture has rapidly expanded in the last decades, representing an important economic activity worldwide; however, bacterial, and viral infections are one of the biggest challenges in aquaculture activity. Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease that affects shrimp farm ponds and occurs during the first 30 days after stocking in shrimp ponds, causing 70-100% mortalities. Microbiota has an important role in developing shrimp aquaculture and disease control. In the present study, pirA and pirB genes were amplified by PCR to confirm AHPND and non-AHPND in shrimp hepatopancreas; samples were sequenced using the MiSeq platform targeting the V3-V4 16S ribosomal (rRNA) hypervariable regions. Results demonstrated a diverse microbiota in the non-AHPND group, mainly composed of phyla Proteobacteria, Firmicutes, and Actinobacteria. Furthermore, Proteobacteria was the dominant phyla in the hepatopancreas infected with AHPND, while Firmicutes tended to decrease. Vibrio was the most abundant at the genus level, prevailing in some genera like Corynebacterium, Weissella, Lactobacillus, Photobacterium, and Geobacillus. Results suggest that AHPND causes dysbiosis in the hepatopancreatic microbiota, and the Firmicutes phylum could require to be restored under such scenario.
{"title":"Screening the microbiota of hepatopancreas associated with toxin genes pirABVP in Penaeus van-namei","authors":"L. A. Ávila-Villa, G. A. Barco-Mendoza, R. Rodríguez-Ramirez, Jesús Daniel Villanueva‐Zayas, Marcel Martínez-Porchas, Alma Guadalupe Villa-Lerma, Belinda Vallejo-Córdoba, Josué Delgado-Domínguez","doi":"10.18633/biotecnia.v26i1.2181","DOIUrl":"https://doi.org/10.18633/biotecnia.v26i1.2181","url":null,"abstract":"Shrimp aquaculture has rapidly expanded in the last decades, representing an important economic activity worldwide; however, bacterial, and viral infections are one of the biggest challenges in aquaculture activity. Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease that affects shrimp farm ponds and occurs during the first 30 days after stocking in shrimp ponds, causing 70-100% mortalities. Microbiota has an important role in developing shrimp aquaculture and disease control. In the present study, pirA and pirB genes were amplified by PCR to confirm AHPND and non-AHPND in shrimp hepatopancreas; samples were sequenced using the MiSeq platform targeting the V3-V4 16S ribosomal (rRNA) hypervariable regions. Results demonstrated a diverse microbiota in the non-AHPND group, mainly composed of phyla Proteobacteria, Firmicutes, and Actinobacteria. Furthermore, Proteobacteria was the dominant phyla in the hepatopancreas infected with AHPND, while Firmicutes tended to decrease. Vibrio was the most abundant at the genus level, prevailing in some genera like Corynebacterium, Weissella, Lactobacillus, Photobacterium, and Geobacillus. Results suggest that AHPND causes dysbiosis in the hepatopancreatic microbiota, and the Firmicutes phylum could require to be restored under such scenario.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"10 3","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138980125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-11DOI: 10.18633/biotecnia.v26i1.2175
Gerardo Álvarez-Hernández, Daraysi Yera-Grillo, Agustín Robles-Morúa, Javier Navarro‐Estupiñan, P. Reyes-Castro, Angélica Aracely Encinas-Cárdenas, Héctor Francisco Duarte-Tagles, M. Candia-Plata
Objective. To characterize the geographic distribution of dengue and to evaluate the spatial autocorrelation with social and climatic determinants at the census-tract level in two medium sized cities in northwestern Mexico. Methods. In this work we apply spatial analysis ecological tools, such as the Moran’s Index and the Local Indicator of Spatial Association (LISA) method, to examine global and local spatial correlation between incidence of dengue, and socioeconomic and climatic factors at the census tract-level. For the analysis of the spatial clustering, the Getis-Ord method was used to find statistically significant hot spots in each city. Results. Overall, a global spatial autocorrelation could not be identified, although local clusters of a high dengue incidence, soil surface temperature ≤ 31 °C and high degree of social marginalization coincide. Discussion. We found that at the census-tract level in urban settings, socially disadvantaged populations showed higher clusters of dengue when compared to areas with better socioeconomic conditions. In the two study sites, a similar spatial pattern was observed when considering public health conditions and its aggregation with physical attributes using spatial analysis techniques, supporting the application of this technique for a better understanding about the dengue distribution in urban areas.
{"title":"Spatial correlation of dengue with socioeconomic status and land temperature in northwest Mexico","authors":"Gerardo Álvarez-Hernández, Daraysi Yera-Grillo, Agustín Robles-Morúa, Javier Navarro‐Estupiñan, P. Reyes-Castro, Angélica Aracely Encinas-Cárdenas, Héctor Francisco Duarte-Tagles, M. Candia-Plata","doi":"10.18633/biotecnia.v26i1.2175","DOIUrl":"https://doi.org/10.18633/biotecnia.v26i1.2175","url":null,"abstract":"Objective. To characterize the geographic distribution of dengue and to evaluate the spatial autocorrelation with social and climatic determinants at the census-tract level in two medium sized cities in northwestern Mexico. Methods. In this work we apply spatial analysis ecological tools, such as the Moran’s Index and the Local Indicator of Spatial Association (LISA) method, to examine global and local spatial correlation between incidence of dengue, and socioeconomic and climatic factors at the census tract-level. For the analysis of the spatial clustering, the Getis-Ord method was used to find statistically significant hot spots in each city. Results. Overall, a global spatial autocorrelation could not be identified, although local clusters of a high dengue incidence, soil surface temperature ≤ 31 °C and high degree of social marginalization coincide. Discussion. We found that at the census-tract level in urban settings, socially disadvantaged populations showed higher clusters of dengue when compared to areas with better socioeconomic conditions. In the two study sites, a similar spatial pattern was observed when considering public health conditions and its aggregation with physical attributes using spatial analysis techniques, supporting the application of this technique for a better understanding about the dengue distribution in urban areas.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"208 4","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138981295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-11DOI: 10.18633/biotecnia.v26i1.2103
Rafael Ramírez Orduña, J. A. Armenta-Quintana, J. M. Ramírez-Orduña, R. Avalos-Castro
The objective of the study was to evaluate the relationship between the preference index (IP) and synchrony (IS) of forage species from Baja California Sur. Samples of the 23 forage species of trees and shrubs were obtained to perform in situ digestibility analyzes using four Creole-Nubian goats. Collections were carried out in winter and spring of 2004 and summer and autumn of 2005. The extent of degradation per hour and total of 24 h was calculated from the amount of OM and PC degraded per hour; these data were used to calculate the IS. The seasonal PI data set was obtained from previously published information. A logistic regression model was used to explain the probability of occurrence of the IP with the IS. The regression model to explain the IP from the IS was significant and predictive for six species, being positive for Adelia virgata, Amaratus palmeri, Haematoxilon brasiletto, Lippia palmeri and Prosopis sp., and negative for Bursera microphyla. For other tree species and legumes in general, the model was significant, but not predictive, therefore, it is necessary to include other factors to fit the model.
这项研究的目的是评估南下加利福尼亚州牧草物种的偏好指数（IP）和同步性（IS）之间的关系。研究人员采集了 23 种乔木和灌木牧草样本，用四只克里奥尔-努比亚山羊进行了现场消化率分析。采集工作于 2004 年冬季和春季以及 2005 年夏季和秋季进行。根据每小时降解的 OM 和 PC 量计算出每小时的降解程度和 24 小时的总降解程度；这些数据用于计算 IS。季节性 PI 数据集来自以前公布的信息。采用逻辑回归模型来解释 IP 与 IS 的发生概率。用 IS 解释 IP 的回归模型对六个物种具有显著的预测意义，对 Adelia virgata、Amaratus palmeri、Haematoxilon brasiletto、Lippia palmeri 和 Prosopis sp.对于其他树种和一般豆科植物，该模型具有显著性，但不具有预测性，因此有必要加入其他因素来拟合该模型。
{"title":"Ruminal degradation synchrony of crude protein and organic matter of forage species as a preference determinant in free-range goats in the Sonoran Desert of Baja California Sur","authors":"Rafael Ramírez Orduña, J. A. Armenta-Quintana, J. M. Ramírez-Orduña, R. Avalos-Castro","doi":"10.18633/biotecnia.v26i1.2103","DOIUrl":"https://doi.org/10.18633/biotecnia.v26i1.2103","url":null,"abstract":" The objective of the study was to evaluate the relationship between the preference index (IP) and synchrony (IS) of forage species from Baja California Sur. Samples of the 23 forage species of trees and shrubs were obtained to perform in situ digestibility analyzes using four Creole-Nubian goats. Collections were carried out in winter and spring of 2004 and summer and autumn of 2005. The extent of degradation per hour and total of 24 h was calculated from the amount of OM and PC degraded per hour; these data were used to calculate the IS. The seasonal PI data set was obtained from previously published information. A logistic regression model was used to explain the probability of occurrence of the IP with the IS. The regression model to explain the IP from the IS was significant and predictive for six species, being positive for Adelia virgata, Amaratus palmeri, Haematoxilon brasiletto, Lippia palmeri and Prosopis sp., and negative for Bursera microphyla. For other tree species and legumes in general, the model was significant, but not predictive, therefore, it is necessary to include other factors to fit the model.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"12 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138979712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.18633/biotecnia.v25i3.2153
Jesús Eduardo Serna Tenorio, A. M. Sotelo-González, R. Reynoso-Camacho, Miriam Aracely Anaya-Loyola, Iza Fernanda Pérez Ramírez
A significant quantity Hibiscus sabdariffa L. calyxes is generated as a by-product during the decoction process commonly used for roselle beverage preparation. We conducted an extensive characterization of polyphenols, organic acids, and antioxidant potential in roselle calyxes, decoction, and their by-product. Roselle calyxes were found to be a rich source of diverse polyphenols, including delphinidin sambubioside, caffeoylquinic acids, hibiscus acid, and citric acid as major components. Importantly, we extracted a significant proportion of these bioactive compounds during the decoction process, resulting in a polyphenol-rich beverage. The used calyces (decoction by-product) retained from 23 % to 140 % of the extractable polyphenols and organic acids found in roselle calyxes. Additionally, due to the leaching of hydrophobic components like soluble dietary fiber and extractable polyphenols and organic acids, the roselle by-product was enriched with non-extractable constituents attached to dietary fiber (126 % - 272 %). Therefore, roselle calyxes and their decoction by-products emerge as promising sources of polyphenols with the potential for use in dietary supplements, alongside the commonly consumed roselle decoction.
{"title":"Comprehensive characterization of the overlooked residue generated during roselle calyxes brewing with potential use as functional ingredient","authors":"Jesús Eduardo Serna Tenorio, A. M. Sotelo-González, R. Reynoso-Camacho, Miriam Aracely Anaya-Loyola, Iza Fernanda Pérez Ramírez","doi":"10.18633/biotecnia.v25i3.2153","DOIUrl":"https://doi.org/10.18633/biotecnia.v25i3.2153","url":null,"abstract":"A significant quantity Hibiscus sabdariffa L. calyxes is generated as a by-product during the decoction process commonly used for roselle beverage preparation. We conducted an extensive characterization of polyphenols, organic acids, and antioxidant potential in roselle calyxes, decoction, and their by-product. Roselle calyxes were found to be a rich source of diverse polyphenols, including delphinidin sambubioside, caffeoylquinic acids, hibiscus acid, and citric acid as major components. Importantly, we extracted a significant proportion of these bioactive compounds during the decoction process, resulting in a polyphenol-rich beverage. The used calyces (decoction by-product) retained from 23 % to 140 % of the extractable polyphenols and organic acids found in roselle calyxes. Additionally, due to the leaching of hydrophobic components like soluble dietary fiber and extractable polyphenols and organic acids, the roselle by-product was enriched with non-extractable constituents attached to dietary fiber (126 % - 272 %). Therefore, roselle calyxes and their decoction by-products emerge as promising sources of polyphenols with the potential for use in dietary supplements, alongside the commonly consumed roselle decoction.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"37 48","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138588901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.18633/biotecnia.v25i3.2127
Luis Ángel Alcalán López, Marcos Alfonso Lastiri Hernández, D. Álvarez-Bernal
El objetivo de esta investigación fue evaluar el efecto de diferentes bioles producidos a partir de plantas halófitas, en la germinación y emergencia de cuatro tipos de hortalizas en condiciones in vitro. Los bioensayos se realizaron en una cámara de germinación, a 25 °C y 65% de humedad relativa durante cinco días. El estudio probó 76 tratamientos, obtenidos a partir de la combinación de seis bioles, cuatro especies vegetales (semillas), tres concentraciones de biol (5, 10 y 20%), y un tratamiento control usando agua destilada estéril, para cada una de las especies. En todas las semillas hortícolas evaluadas se observaron reducciones significativas (p ≤ 0.05) en el porcentaje relativo de germinación, crecimiento relativo de la radícula, índice de germinación y longitud total de las plántulas, con relación a los testigos a medida que aumentaban las concentraciones de biol establecidas; fueron el T6 (biol de S. verrucosum), T4 (biol de H. Curassavicum) y T5 (biol de F. trinervia) los que mostraron los niveles más bajos de germinación y emergencia en general. Sin embargo, en todas las semillas, con el uso de concentraciones de biol al 5%, no se observaron niveles de fitotoxicidad, ya que alcanzaron porcentajes de germinación superiores al 85.55%.
{"title":"Efecto de diferentes bioles, obtenidos a partir de halófitas, en la germinación y crecimiento de cuatro variedades de hortalizas","authors":"Luis Ángel Alcalán López, Marcos Alfonso Lastiri Hernández, D. Álvarez-Bernal","doi":"10.18633/biotecnia.v25i3.2127","DOIUrl":"https://doi.org/10.18633/biotecnia.v25i3.2127","url":null,"abstract":"El objetivo de esta investigación fue evaluar el efecto de diferentes bioles producidos a partir de plantas halófitas, en la germinación y emergencia de cuatro tipos de hortalizas en condiciones in vitro. Los bioensayos se realizaron en una cámara de germinación, a 25 °C y 65% de humedad relativa durante cinco días. El estudio probó 76 tratamientos, obtenidos a partir de la combinación de seis bioles, cuatro especies vegetales (semillas), tres concentraciones de biol (5, 10 y 20%), y un tratamiento control usando agua destilada estéril, para cada una de las especies. En todas las semillas hortícolas evaluadas se observaron reducciones significativas (p ≤ 0.05) en el porcentaje relativo de germinación, crecimiento relativo de la radícula, índice de germinación y longitud total de las plántulas, con relación a los testigos a medida que aumentaban las concentraciones de biol establecidas; fueron el T6 (biol de S. verrucosum), T4 (biol de H. Curassavicum) y T5 (biol de F. trinervia) los que mostraron los niveles más bajos de germinación y emergencia en general. Sin embargo, en todas las semillas, con el uso de concentraciones de biol al 5%, no se observaron niveles de fitotoxicidad, ya que alcanzaron porcentajes de germinación superiores al 85.55%.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"39 45","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138588595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-06DOI: 10.18633/biotecnia.v26i1.2128
Rey David Vargas Sánchez, Brisa del Mar Torres Martínez, Gastón Ramón Torrescano Urrutia, A. Sánchez-Escalante
Coffee fruit processing residues have been proposed to prevent meat quality loss. This study aimed to evaluate the effectiveness of coffee silverskin aqueous extract (CSE) on the oxidative stability of a pork meat homogenate. CSE was subjected to polyphenols determination (total phenol, flavonoids, and caffeoylquinic acid contents) and antiradical and reducing power assays. In addition, raw pork meat homogenates were divided into four treatments (CN, control or without antioxidant; T1 and T2, CSE at 250 and 500 ppm, respectively; BHT, synthetic antioxidant at 500 ppm), oxidized during 1 h with potassium ferrocyanide (0, 0.5, and 1.0%, w/v), and were subjected to meat quality evaluation (pH, lipid oxidation, color, and metmyoglobin content). Results demonstrated that CSE is an important source of polyphenols with antioxidant activity, and their incorporation in a raw pork meat homogenate led to reduce pH values, lipid oxidation, and metmyoglobin content, as well as decreased color changes (p < 0.05). These results suggest that CSE has great potential as an antioxidant additive for meat products.
{"title":"Utilization of coffee silverskin aqueous extract for improving the oxidative stability of pork meat homogenates","authors":"Rey David Vargas Sánchez, Brisa del Mar Torres Martínez, Gastón Ramón Torrescano Urrutia, A. Sánchez-Escalante","doi":"10.18633/biotecnia.v26i1.2128","DOIUrl":"https://doi.org/10.18633/biotecnia.v26i1.2128","url":null,"abstract":"Coffee fruit processing residues have been proposed to prevent meat quality loss. This study aimed to evaluate the effectiveness of coffee silverskin aqueous extract (CSE) on the oxidative stability of a pork meat homogenate. CSE was subjected to polyphenols determination (total phenol, flavonoids, and caffeoylquinic acid contents) and antiradical and reducing power assays. In addition, raw pork meat homogenates were divided into four treatments (CN, control or without antioxidant; T1 and T2, CSE at 250 and 500 ppm, respectively; BHT, synthetic antioxidant at 500 ppm), oxidized during 1 h with potassium ferrocyanide (0, 0.5, and 1.0%, w/v), and were subjected to meat quality evaluation (pH, lipid oxidation, color, and metmyoglobin content). Results demonstrated that CSE is an important source of polyphenols with antioxidant activity, and their incorporation in a raw pork meat homogenate led to reduce pH values, lipid oxidation, and metmyoglobin content, as well as decreased color changes (p < 0.05). These results suggest that CSE has great potential as an antioxidant additive for meat products.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"64 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138595488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-06DOI: 10.18633/biotecnia.v26i1.2028
Emir Alejandro Hernández Gómez, Tomás Jesús Madera Santana, Patricia Quintana Owen, Elizabeth Ortiz Vázquez, Jesús Ramón Sierra, María de Lourdes Vargas y Vargas
Las películas comestibles (PCs) presentan una alternativa para incrementar la vida útil de frutas y hortalizas al servir como barrera para la transferencia de oxígeno y humedad; y al mismo tiempo, permitir la liberación de compuestos con actividad antioxidante y antimicrobiana hacia el alimento. En el presente estudio se formuló y caracterizó películas a base de alginato de sodio (A) a distintas concentraciones de miel de melipona (M). Las PCs se formularon con una concentración de 2 % de A y 6 concentraciones diferentes de M (0 hasta 1.6 % p/v) y se les evaluó las propiedades físicas, de barrera, mecánicas, ópticas, estructurales, morfológicas y bioactivas. En las propiedades físicas, las películas presentaron un incremento en el espesor y la densidad, y una disminución en el porcentaje de humedad al incrementar el contenido de miel. Las PCs mostraron un efecto plastificante observado en la mayor concentración de M reflejando un aumento de hasta 9 % en el porcentaje de elongación, una disminución en el esfuerzo a la tensión y módulo elástico de 18.36 MPa y 283.88 MPa, respectivamente. La formulación con 1.6 % de M mostró las propiedades fisicoquímicas adecuadas y propiedades plastificantes para ser usada como PCs.
可食用薄膜(PCs)是延长水果和蔬菜保质期的一种选择，因为它可以作为氧气和水分转移的屏障;同时，允许释放具有抗氧化和抗菌活性的化合物到食物中。本研究提出了影片的特点是海藻酸钠(a)不同melipona蜂蜜的浓度(M)。个人电脑提出了集中2 % a和M的6种不同的浓度(0到1.6 % p / v)和他们的物理特性,评价、morfológicas和结构性障碍、机械、光学活性。在物理性能方面，随着蜂蜜含量的增加，薄膜的厚度和密度增加，水分百分比降低。在M浓度较高的情况下，PCs表现出塑化效应，伸长率增加9%，抗拉应力和弹性模量分别降低18.36 MPa和283.88 MPa。含1.6% M的配方具有适当的理化性能和增塑剂性能，可作为PCs使用。
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Pub Date : 2023-11-14DOI: 10.18633/biotecnia.v25i3.2115
G. Velázquez, Elena Ortega Morente, Antonio Cobo Molinos, Beatriz Perez Armendariz
Los alimentos contaminados por patógenos forman biopelículas con una alta resistencia a biocidas, desinfectantes y antibióticos. Los extractos vegetales pueden ser una alternativa potencial para combatirlos. Este estudio evaluó la actividad antimicrobiana y anti biopelícula de un extracto vegetal (Sambucus canadensis) utilizado ampliamente en comunidades indígenas de México. El trabajo se realizó con bacterias aisladas de alimentos orgánicos. Las pruebas antimicrobianas mostraron actividad en todas las cepas evaluadas principalmente en S. aureus 976, S. entérica 915, E. coli 47553 (CMI 65.1 y 50 µg/mL) respectivamente. Respecto a la obstrucción en la formación de biopelícula se encontraron resultados significativos menores a 35 % a la concentración más baja 0.01 µg/mL frente a las cepas S. aureus CECT 976 y E. coli 47553. Respecto a los resultados en la disgregación del biopelicula las bacterias E. coli CECT 4757, S. aureus CECT 976 y S. aureus CECT 4465 lograron hasta un 36 % de disgregación en la concentración más baja de 0.01 µg/mL siendo estadísticamente significativo respecto al control (p < 0.001). Los resultados indican que el extracto vegetal tiene un alto potencial de actividad antibiopelícula frente a bacterias que afectan la inocuidad de los alimentos y plantean riegos para la salud de las personas.
{"title":"Actividad antimicrobiana y antibiopelicula del extracto vegetal Sambucus canadensis en bacterias patogenas transmitidas por alimentos","authors":"G. Velázquez, Elena Ortega Morente, Antonio Cobo Molinos, Beatriz Perez Armendariz","doi":"10.18633/biotecnia.v25i3.2115","DOIUrl":"https://doi.org/10.18633/biotecnia.v25i3.2115","url":null,"abstract":"Los alimentos contaminados por patógenos forman biopelículas con una alta resistencia a biocidas, desinfectantes y antibióticos. Los extractos vegetales pueden ser una alternativa potencial para combatirlos. Este estudio evaluó la actividad antimicrobiana y anti biopelícula de un extracto vegetal (Sambucus canadensis) utilizado ampliamente en comunidades indígenas de México. El trabajo se realizó con bacterias aisladas de alimentos orgánicos. Las pruebas antimicrobianas mostraron actividad en todas las cepas evaluadas principalmente en S. aureus 976, S. entérica 915, E. coli 47553 (CMI 65.1 y 50 µg/mL) respectivamente. Respecto a la obstrucción en la formación de biopelícula se encontraron resultados significativos menores a 35 % a la concentración más baja 0.01 µg/mL frente a las cepas S. aureus CECT 976 y E. coli 47553. Respecto a los resultados en la disgregación del biopelicula las bacterias E. coli CECT 4757, S. aureus CECT 976 y S. aureus CECT 4465 lograron hasta un 36 % de disgregación en la concentración más baja de 0.01 µg/mL siendo estadísticamente significativo respecto al control (p < 0.001). Los resultados indican que el extracto vegetal tiene un alto potencial de actividad antibiopelícula frente a bacterias que afectan la inocuidad de los alimentos y plantean riegos para la salud de las personas.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"53 7","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139277737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-13DOI: 10.18633/biotecnia.v25i3.1991
Deanna Esquivel-Figueroa, Martin Ernesto Tiznado-Hernández, María Auxiliadora Islas-Osuna, María Fernanda Lazo-Javalera, Marisela Rivera-Domínguez
La vid es uno de los principales cultivos del mundo y el estado de Sonora es el mayor productor de México. Este cultivo está en constante peligro por diversos factores bióticos y abióticos, de ahí la importancia de su conservación. La crioconservación es idónea para este cultivo, pero puede provocar alteraciones fisiológicas, moleculares y bioquímicas, afectando la viabilidad y la regeneración. Por lo anterior, en este trabajo se planteó analizar el efecto de las etapas del protocolo de crioconservación sobre la viabilidad y regeneración de embriones cigóticos de vid. La viabilidad del tejido (V) y la regeneración de plántulas (RP), mostraron que la PVS2 ejerce protección a los embriones (V:85%, RP:60%), mientras que la exposición combinada a la solución PVS2 y nitrógeno líquido (NL) ocasionó diminución de la viabilidad y regeneración (V:68%; RP: 2%). Un efecto más drástico se observó cuando el tejido fue expuesto a PVS2+NL y recalentamiento (RC) (V:68%; RP:0%). Sin embargo, la viabilidad y la regeneración se recuperó cuando el tejido se sometió a PVS2+NL+RC y solución de descarga (SD) (V:92%; RP:60%). Se concluye que la utilización de la solución de descarga es fundamental para evitar daños a los tejidos debido a las diferentes etapas de crioconservación.
{"title":"Etapas del protocolo de crioconservación y su efecto sobre la viabilidad y regeneración de embriones cigóticos de vid (Vitis vinífera L.)","authors":"Deanna Esquivel-Figueroa, Martin Ernesto Tiznado-Hernández, María Auxiliadora Islas-Osuna, María Fernanda Lazo-Javalera, Marisela Rivera-Domínguez","doi":"10.18633/biotecnia.v25i3.1991","DOIUrl":"https://doi.org/10.18633/biotecnia.v25i3.1991","url":null,"abstract":"La vid es uno de los principales cultivos del mundo y el estado de Sonora es el mayor productor de México. Este cultivo está en constante peligro por diversos factores bióticos y abióticos, de ahí la importancia de su conservación. La crioconservación es idónea para este cultivo, pero puede provocar alteraciones fisiológicas, moleculares y bioquímicas, afectando la viabilidad y la regeneración. Por lo anterior, en este trabajo se planteó analizar el efecto de las etapas del protocolo de crioconservación sobre la viabilidad y regeneración de embriones cigóticos de vid. La viabilidad del tejido (V) y la regeneración de plántulas (RP), mostraron que la PVS2 ejerce protección a los embriones (V:85%, RP:60%), mientras que la exposición combinada a la solución PVS2 y nitrógeno líquido (NL) ocasionó diminución de la viabilidad y regeneración (V:68%; RP: 2%). Un efecto más drástico se observó cuando el tejido fue expuesto a PVS2+NL y recalentamiento (RC) (V:68%; RP:0%). Sin embargo, la viabilidad y la regeneración se recuperó cuando el tejido se sometió a PVS2+NL+RC y solución de descarga (SD) (V:92%; RP:60%). Se concluye que la utilización de la solución de descarga es fundamental para evitar daños a los tejidos debido a las diferentes etapas de crioconservación.","PeriodicalId":8876,"journal":{"name":"Biotecnia","volume":"46 18","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136282376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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