Expression analysis of POSTN gene in ovine follicles

IF 1.2 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Canadian Journal of Animal Science Pub Date : 2022-10-26 DOI:10.1139/cjas-2021-0036
Jiapeng Lin, Chunjie Liu, Liqin Wang, Ying Chen, Xiaolin Li, Yangsheng Wu, Juncheng Huang
{"title":"Expression analysis of POSTN gene in ovine follicles","authors":"Jiapeng Lin, Chunjie Liu, Liqin Wang, Ying Chen, Xiaolin Li, Yangsheng Wu, Juncheng Huang","doi":"10.1139/cjas-2021-0036","DOIUrl":null,"url":null,"abstract":"Abstract The purpose of this study was to explore the potential expression regularity of POSTN in ovarian tissue. In this study, the uterus, ovarian follicles, heart, liver, spleen, lung, kidney, and muscle tissues of Merino sheep (10) were collected and detected by Western blot, real-time quantitative polymerase chain reaction, immunohistofluorescence, and fluorescence staining, respectively. The expression of POSTN in various organizations was analyzed. The results showed that the POSTN in Merino sheep had close homology with Bos mutus and Bos taurus. The expression of POSTN was detected in the uterus, follicle, heart, liver, spleen, lung, kidney, and muscle tissue, among which the expression of POSTN was highest in ovarian tissue. In addition, the expression of POSTN gradually increased with the increase of follicle diameter, among which POSTN was highly expressed in the granulosa cells (GCs) of follicles. Meanwhile, POSTN were distributed throughout the nucleus and cytoplasm of GCs, suggesting that POSTN may be involved in the regulation of follicle development.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian Journal of Animal Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1139/cjas-2021-0036","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

Abstract

Abstract The purpose of this study was to explore the potential expression regularity of POSTN in ovarian tissue. In this study, the uterus, ovarian follicles, heart, liver, spleen, lung, kidney, and muscle tissues of Merino sheep (10) were collected and detected by Western blot, real-time quantitative polymerase chain reaction, immunohistofluorescence, and fluorescence staining, respectively. The expression of POSTN in various organizations was analyzed. The results showed that the POSTN in Merino sheep had close homology with Bos mutus and Bos taurus. The expression of POSTN was detected in the uterus, follicle, heart, liver, spleen, lung, kidney, and muscle tissue, among which the expression of POSTN was highest in ovarian tissue. In addition, the expression of POSTN gradually increased with the increase of follicle diameter, among which POSTN was highly expressed in the granulosa cells (GCs) of follicles. Meanwhile, POSTN were distributed throughout the nucleus and cytoplasm of GCs, suggesting that POSTN may be involved in the regulation of follicle development.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
POSTN基因在绵羊卵泡中的表达分析
摘要本研究旨在探讨POSTN在卵巢组织中的潜在表达规律。在本研究中,收集美利奴羊(10)的子宫、卵泡、心、肝、脾、肺、肾和肌肉组织,并分别通过蛋白质印迹、实时定量聚合酶链式反应、免疫组织荧光和荧光染色进行检测。分析了POSTN在不同组织中的表达。结果表明,美利奴羊的POSTN基因与麋鹿和牛头犬具有密切的同源性。POSTN在子宫、卵泡、心、肝、脾、肺、肾和肌肉组织中表达,其中POSTN在卵巢组织中表达最高。此外,POSTN的表达随着卵泡直径的增加而逐渐增加,其中POSTN在卵泡颗粒细胞(GC)中高表达。同时,POSTN分布于GC的细胞核和细胞质中,提示POSTN可能参与卵泡发育的调控。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Canadian Journal of Animal Science
Canadian Journal of Animal Science 农林科学-奶制品与动物科学
CiteScore
2.30
自引率
0.00%
发文量
51
审稿时长
6 months
期刊介绍: Published since 1957, this quarterly journal contains new research on all aspects of animal agriculture and animal products, including breeding and genetics; cellular and molecular biology; growth and development; meat science; modelling animal systems; physiology and endocrinology; ruminant nutrition; non-ruminant nutrition; and welfare, behaviour, and management. It also publishes reviews, letters to the editor, abstracts of technical papers presented at the annual meeting of the Canadian Society of Animal Science, and occasionally conference proceedings.
期刊最新文献
Simulation of loading and unloading through ramps of different configuration: effects on the ease of handling and physiological response of pigs of two slaughter weights Comparative impact of conventional and alternative gut health management programs on plasma and tibia attributes in broiler chickens raised in commercial and research settings Characterization of First Cut Alfalfa and Grass Silage Management Practices on Canadian Dairy Farms A Review of Foot-Related Lameness in Feedlot Cattle Complete replacement of soybean meal with black soldier fly larvae meal in feeding program for broiler chickens from placement through to 49 days of age: impact on gastrointestinal, breast, skeletal, plasma, and litter attributes
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1